HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity

High-throughput single-cell methods have uncovered substantial heterogeneity in the pool of hematopoietic stem and progenitor cells (HSPCs), but how much instruction is inherited by offspring from their heterogeneous ancestors remains unanswered. Using a method that enables simultaneous determinatio...

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Published in:eLife Vol. 10
Main Authors: Tak, Tamar, Prevedello, Giulio, Simon, Gaël, Paillon, Noémie, Benlabiod, Camélia, Marty, Caroline, Plo, Isabelle, Duffy, Ken R, Perié, Leïla
Format: Journal Article
Language:English
Published: England eLife Sciences Publications Ltd 18.05.2021
eLife Sciences Publication
eLife Sciences Publications, Ltd
Subjects:
Antibodies
Blood
Bone marrow transplantation
cell differentiation
Cell division
cell proliferation
Cell surface
concordance
Experiments
Generations
haematopoietic stem
Hematopoietic stem cells
Physics
progenitor cell
Progenitor cells
Stem cells
Stem Cells and Regenerative Medicine
Surface markers
mouse
stem cells
regenerative medicine
ISSN:2050-084X, 2050-084X
Online Access:Get full text
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Summary:High-throughput single-cell methods have uncovered substantial heterogeneity in the pool of hematopoietic stem and progenitor cells (HSPCs), but how much instruction is inherited by offspring from their heterogeneous ancestors remains unanswered. Using a method that enables simultaneous determination of common ancestor, division number, and differentiation status of a large collection of single cells, our data revealed that murine cells that derived from a common ancestor had significant similarities in their division progression and differentiation outcomes. Although each family diversifies, the overall collection of cell types observed is composed of homogeneous families. Heterogeneity between families could be explained, in part, by differences in ancestral expression of cell surface markers. Our analyses demonstrate that fate decisions of cells are largely inherited from ancestor cells, indicating the importance of common ancestor effects. These results may have ramifications for bone marrow transplantation and leukemia, where substantial heterogeneity in HSPC behavior is observed.
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These authors contributed equally to this work.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.60624