Use of PCR to determine Toxoplasma gondii in milk samples from camels (Camelus dromedarius), cattle (Bos taurus) and buffalos (Bubalus bubalis) in East Azarbaijan province, Iran

Background Toxoplasmosis as a zoonotic condition is developed by an intracellular protozoan parasite Toxoplasma gondii from the Apicomplexa phylum, which imposes economic losses on herds of animals and severe complications in immunocompromised people and pregnant women. This infectious disease can b...

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Published in:Veterinary medicine and science Vol. 9; no. 1; pp. 400 - 404
Main Authors: Asiyabi Aghdam, Somayyeh, Hajipour, Nasser, Moosavy, Mir‐Hassan
Format: Journal Article
Language:English
Published: England John Wiley & Sons, Inc 01.01.2023
John Wiley and Sons Inc
Wiley
Subjects:
Animals
B1 gene
Bos taurus
Bubalus bubalis
buffalo
Buffalo milk
Buffaloes
camel
Camel's milk
Camelus
Camelus dromedarius
Cats
Cattle
Cattle Diseases
cow
Cow's milk
DNA
Female
Food contamination
Humans
Infections
Infectious diseases
Iran - epidemiology
Milk
Milking
Oocysts
Original
Polymerase chain reaction
Polymerase Chain Reaction - veterinary
Pregnancy
Protozoa
RUMINANTS
Sheep
Toxoplasma - genetics
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis, Animal - epidemiology
Toxoplasmosis, Animal - parasitology
Iran
Germany
milk
cow
Toxoplasma gondii
camel
buffalo
ISSN:2053-1095, 2053-1095
Online Access:Get full text
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Summary:Background Toxoplasmosis as a zoonotic condition is developed by an intracellular protozoan parasite Toxoplasma gondii from the Apicomplexa phylum, which imposes economic losses on herds of animals and severe complications in immunocompromised people and pregnant women. This infectious disease can be transmitted to human beings from the contaminated unpasteurized milk, uncooked meat, water and food contaminated with sporulated oocysts and transplacental transmission. Objectives This study amid to determine T. gondii DNA in camel, buffalo and cow milks in using the PCR method based on the B1 gene. Methods A total of 100 milk samples, including 55 cows, 30 buffalos and 15 camels, were collected from different regions of north‐western using direct milking and then transferred to the Food and Aquatic Health Laboratory under refrigerated conditions. Results The results showed that out of 100 milk samples examined, 5 samples (5%) were contaminated, and T. gondii DNA was detected in the milk samples of 2 (3.63%) cows, 1 (3.33%) buffalos and 2 (13.33%) camels, respectively. Conclusions Our findings reveal that raw milk contaminated with T. gondii can be an important route of transmission of infection for human beings. Toxoplasma gondii is a food‐borne zoonotic parasite that infects humans. The results demonstrate the presence of the DNA of T. gondii in buffalo, cow and camel's milk. Consumption of raw milk of different animals can be a risk factor for human health. To prevent infection with T. gondii, it is recommended to boil milk before consumption.
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ISSN:2053-1095
2053-1095
DOI:10.1002/vms3.1047