The dysregulation of metabolic pathways and induction of the pentose phosphate pathway in renal ischaemia–reperfusion injury

Lipid accumulation is associated with various forms of acute renal injury; however, the causative factors and pathways underpinning this lipid accumulation have not been thoroughly investigated. In this study, we performed lipidomic profiling of renal tissue following ischaemia–reperfusion injury (I...

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Veröffentlicht in:The Journal of pathology Jg. 253; H. 4; S. 404 - 414
Hauptverfasser: Scantlebery, Angelique ML, Tammaro, Alessandra, Mills, James D, Rampanelli, Elena, Kors, Lotte, Teske, Gwendoline J, Butter, Loes M, Liebisch, Gerhard, Schmitz, Gerd, Florquin, Sandrine, Leemans, Jaklien C, Roelofs, Joris JTH
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Chichester, UK John Wiley & Sons, Ltd 01.04.2021
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Schlagworte:
Cholesterol
Energy metabolism
fatty acid beta‐oxidation
Fatty acids
Glycolysis
ischaemia–reperfusion injury
Ischemia
kidney
Kidneys
lipid accumulation
Lipid metabolism
Lipids
Metabolic pathways
Metabolism
Mitochondria
Original Paper
Original Papers
Oxidation
Pentose phosphate pathway
Phospholipids
Reperfusion
Sphingolipids
kidney
lipid metabolism
fatty acid beta-oxidation
ischaemia-reperfusion injury
lipid accumulation
energy metabolism
pentose phosphate pathway
ISSN:0022-3417, 1096-9896, 1096-9896
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Zusammenfassung:Lipid accumulation is associated with various forms of acute renal injury; however, the causative factors and pathways underpinning this lipid accumulation have not been thoroughly investigated. In this study, we performed lipidomic profiling of renal tissue following ischaemia–reperfusion injury (IRI). We identified a significant accumulation of cholesterol and specific phospholipids and sphingolipids in kidneys 24 h after IRI. In light of these findings, we hypothesised that pathways involved in lipid metabolism may also be altered. Through the analysis of published microarray data, generated from sham and ischaemic kidneys, we identified nephron‐specific metabolic pathways affected by IRI and validated these findings in ischaemic renal tissue. In silico analysis revealed the downregulation of several energy and lipid metabolism pathways, including mitochondrial fatty acid beta‐oxidation (FAO), peroxisomal lipid metabolism, fatty acid (FA) metabolism, and glycolysis. The pentose phosphate pathway (PPP), which is fuelled by glycolysis, was the only metabolic pathway that was upregulated 24 h following IRI. In this study, we describe the effect of renal IRI on metabolic pathways and how this contributes to lipid accumulation. © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland.
Bibliographie:No conflicts of interest were declared.
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ISSN:0022-3417
1096-9896
1096-9896
DOI:10.1002/path.5605