A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum

Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant...

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Vydáno v:The New phytologist Ročník 217; číslo 2; s. 739 - 755
Hlavní autoři: Yang, Guogen, Tang, Liguang, Gong, Yingdi, Xie, Jiatao, Fu, Yanping, Jiang, Daohong, Li, Guoqing, Collinge, David B., Chen, Weidong, Cheng, Jiasen
Médium: Journal Article
Jazyk:angličtina
Vydáno: England New Phytologist Trust 01.01.2018
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Témata:
Alternaria brassicicola
Amino Acid Sequence
Apoplast
Arabidopsis - genetics
Arabidopsis - microbiology
Arabidopsis Proteins - metabolism
Ascomycota - genetics
Ascomycota - pathogenicity
Botrytis cinerea
Cell Death
Clonal deletion
Disease Resistance - genetics
Fungal Proteins - chemistry
Fungal Proteins - metabolism
Fungi
Gene Expression Regulation, Fungal
Gene Expression Regulation, Plant
Golovinomyces orontii
Host plants
Host-Pathogen Interactions
Immunoprecipitation
Nicotiana - genetics
Nicotiana - microbiology
Nicotiana benthamiana
Pathogens
Phylogeny
phytotoxicity
plant defense
Plant Diseases - genetics
Plant Diseases - microbiology
plant pathogens
PR1
precipitin tests
Protein Binding
Proteins
Salicylic acid
Salicylic Acid - metabolism
Sclerotinia sclerotiorum
Signal transduction
Signaling
SsCP1
Transgenic plants
two hybrid system techniques
Virulence
Yeast
Yeasts
Sclerotinia sclerotiorum
SsCP1
plant defense
virulence
PR1
ISSN:0028-646X, 1469-8137, 1469-8137
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Shrnutí:Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant infection, and deletion of Sscp1 significantly reduced virulence. SsCP1 could induce significant cell death when expressed in Nicotiana benthamiana. Using yeast two-hybrid, GST pull-down, co-immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection by S. sclerotiorum. Overexpressing PR1 enhanced resistance to the wild-type strain, but not to the Sscp1 knockout strain of S. sclerotiorum. Sscp1-expressing transgenic plants showed increased concentrations of salicylic acid (SA) and higher levels of resistance to several plant pathogens (namely Botrytis cinerea, Alternaria brassicicola and Golovinomyces orontii). Our results suggest that SsCP1 is important for virulence of S. sclerotiorum and that it can be recognized by plants to trigger plant defense responses. Our results also suggest that the SA signaling pathway is involved in CP-mediated plant defense.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0028-646X
1469-8137
1469-8137
DOI:10.1111/nph.14842