Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces

Bats are natural hosts for a large variety of zoonotic viruses. This study aimed to describe the range of bat viromes, including viruses from mammals, insects, fungi, plants, and phages, in 11 insectivorous bat species (216 bats in total) common in six provinces of China. To analyze viromes, we used...

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Bibliographic Details
Published in:Journal of virology Vol. 86; no. 20; p. 10999
Main Authors: Wu, Zhiqiang, Ren, Xianwen, Yang, Li, Hu, Yongfeng, Yang, Jian, He, Guimei, Zhang, Junpeng, Dong, Jie, Sun, Lilian, Du, Jiang, Liu, Liguo, Xue, Ying, Wang, Jianmin, Yang, Fan, Zhang, Shuyi, Jin, Qi
Format: Journal Article
Language:English
Published: United States 01.10.2012
Subjects:
Animals
Bacteriophages - classification
China
Chiroptera - virology
Fungi - virology
Genetic Variation
Genome, Viral
Insect Viruses - classification
Insect Viruses - genetics
Insect Viruses - isolation & purification
Insecta - virology
Mammals - virology
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Phylogeny
Plant Viruses - classification
Plant Viruses - genetics
Plant Viruses - isolation & purification
Plants - virology
Viruses - classification
Viruses - genetics
Viruses - isolation & purification
ISSN:1098-5514, 1098-5514
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Summary:Bats are natural hosts for a large variety of zoonotic viruses. This study aimed to describe the range of bat viromes, including viruses from mammals, insects, fungi, plants, and phages, in 11 insectivorous bat species (216 bats in total) common in six provinces of China. To analyze viromes, we used sequence-independent PCR amplification and next-generation sequencing technology (Solexa Genome Analyzer II; Illumina). The viromes were identified by sequence similarity comparisons to known viruses. The mammalian viruses included those of the Adenoviridae, Herpesviridae, Papillomaviridae, Retroviridae, Circoviridae, Rhabdoviridae, Astroviridae, Flaviridae, Coronaviridae, Picornaviridae, and Parvovirinae; insect viruses included those of the Baculoviridae, Iflaviridae, Dicistroviridae, Tetraviridae, and Densovirinae; fungal viruses included those of the Chrysoviridae, Hypoviridae, Partitiviridae, and Totiviridae; and phages included those of the Caudovirales, Inoviridae, and Microviridae and unclassified phages. In addition to the viruses and phages associated with the insects, plants, and bacterial flora related to the diet and habitation of bats, we identified the complete or partial genome sequences of 13 novel mammalian viruses. These included herpesviruses, papillomaviruses, a circovirus, a bocavirus, picornaviruses, a pestivirus, and a foamy virus. Pairwise alignments and phylogenetic analyses indicated that these novel viruses showed little genetic similarity with previously reported viruses. This study also revealed a high prevalence and diversity of bat astroviruses and coronaviruses in some provinces. These findings have expanded our understanding of the viromes of bats in China and hinted at the presence of a large variety of unknown mammalian viruses in many common bat species of mainland China.
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ISSN:1098-5514
1098-5514
DOI:10.1128/JVI.01394-12