Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications...

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Published in:	Frontiers in genetics Vol. 12; p. 673286
Main Authors:	Duan, Li, Ouyang, Kan, Xu, Xiao, Xu, Limei, Wen, Caining, Zhou, Xiaoying, Qin, Zhuan, Xu, Zhiyi, Sun, Wei, Liang, Yujie
Format:	Journal Article
Language:	English
Published:	Frontiers Media S.A 12.05.2021
Subjects:	CRISPR/Cas9 delivery exosome Genetics modification nanocarriers
ISSN:	1664-8021, 1664-8021
Online Access:	Get full text
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Abstract	The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery.
AbstractList	The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery. The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery.The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery.
Author	Sun, Wei Liang, Yujie Xu, Xiao Ouyang, Kan Wen, Caining Qin, Zhuan Xu, Zhiyi Zhou, Xiaoying Duan, Li Xu, Limei
AuthorAffiliation	1 Department of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital , Shenzhen , China 2 Shenzhen Institute of Geriatrics , Shenzhen , China 3 Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital , Shenzhen , China 4 Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health , Shenzhen , China
AuthorAffiliation_xml	– name: 4 Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health , Shenzhen , China – name: 1 Department of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital , Shenzhen , China – name: 2 Shenzhen Institute of Geriatrics , Shenzhen , China – name: 3 Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital , Shenzhen , China
Author_xml	– sequence: 1 givenname: Li surname: Duan fullname: Duan, Li – sequence: 2 givenname: Kan surname: Ouyang fullname: Ouyang, Kan – sequence: 3 givenname: Xiao surname: Xu fullname: Xu, Xiao – sequence: 4 givenname: Limei surname: Xu fullname: Xu, Limei – sequence: 5 givenname: Caining surname: Wen fullname: Wen, Caining – sequence: 6 givenname: Xiaoying surname: Zhou fullname: Zhou, Xiaoying – sequence: 7 givenname: Zhuan surname: Qin fullname: Qin, Zhuan – sequence: 8 givenname: Zhiyi surname: Xu fullname: Xu, Zhiyi – sequence: 9 givenname: Wei surname: Sun fullname: Sun, Wei – sequence: 10 givenname: Yujie surname: Liang fullname: Liang, Yujie
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ContentType	Journal Article
Copyright	Copyright © 2021 Duan, Ouyang, Xu, Xu, Wen, Zhou, Qin, Xu, Sun and Liang. Copyright © 2021 Duan, Ouyang, Xu, Xu, Wen, Zhou, Qin, Xu, Sun and Liang. 2021 Duan, Ouyang, Xu, Xu, Wen, Zhou, Qin, Xu, Sun and Liang
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Snippet	The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool...
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Title	Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing
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