Using terminal restriction fragment length polymorphism (T-RFLP) to identify mycorrhizal fungi: a methods review

Terminal restriction fragment length polymorphism (T-RFLP) is an increasingly widely used technique in mycorrhizal ecology. In this paper, we review the technique as it is used to identify species of mycorrhizal fungi and distinguish two different versions of the technique: peak-profile T-RFLP (the...

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Bibliographic Details
Published in:	Mycorrhiza Vol. 17; no. 4; pp. 259 - 270
Main Authors:	Dickie, I. A, FitzJohn, R. G
Format:	Journal Article
Language:	English
Published:	Berlin Berlin/Heidelberg : Springer-Verlag 01.06.2007 Springer Springer Nature B.V
Subjects:	Bacteria Biological and medical sciences classification Cloning Colony Count, Microbial Databases, Genetic DNA Primers DNA Restriction Enzymes DNA, Fungal DNA, Fungal - genetics DNA, Ribosomal Spacer ectomycorrhizae Fundamental and applied biological sciences. Psychology Fungi genetics methods Microbiology Molecular identification Mycological Typing Techniques Mycological Typing Techniques - methods mycorrhizae Mycorrhizae - classification Mycorrhizae - genetics mycorrhizal fungi Parasitism and symbiosis Plant physiology and development Polymerase Chain Reaction Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length polyphyly restriction fragment length polymorphism risk species identification Species Specificity Symbiosis Terminal restriction fragment length polymorphism (T-RFLP) vesicular arbuscular mycorrhizae Fungi Endomycorrhiza Restriction fragment length polymorphism Mycorrhiza Ectomycorrhiza Replication Review Arbuscular mycorrhiza Molecular identification,Terminal restriction fragment length polymorphism (T-RFLP) Thallophyta
ISSN:	0940-6360, 1432-1890
Online Access:	Get full text
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