Inclusion bodies are a site of ebolavirus replication

Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological significance has been debated, and they have been suggested to be aggregates of viral proteins without any further biological function. However...

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Vydáno v:	Journal of virology Ročník 86; číslo 21; s. 11779
Hlavní autoři:	Hoenen, Thomas, Shabman, Reed S, Groseth, Allison, Herwig, Astrid, Weber, Michaela, Schudt, Gordian, Dolnik, Olga, Basler, Christopher F, Becker, Stephan, Feldmann, Heinz
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 01.11.2012
Témata:	Animals Artificial Gene Fusion Cell Line Ebolavirus - physiology Genes, Reporter Humans Inclusion Bodies, Viral - virology Luminescent Proteins - analysis Luminescent Proteins - genetics Microscopy, Fluorescence Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics Red Fluorescent Protein RNA-Dependent RNA Polymerase - genetics Transfection Viral Proteins - genetics Virus Replication
ISSN:	1098-5514, 1098-5514
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Abstract	Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological significance has been debated, and they have been suggested to be aggregates of viral proteins without any further biological function. However, recent data for other viruses that produce similar structures have suggested that inclusion bodies might be involved in genome replication and transcription. In order to study filovirus inclusion bodies, we fused mCherry to the ebolavirus polymerase L, which is found in inclusion bodies. The resulting L-mCherry fusion protein was functional in minigenome assays and incorporated into virus-like particles. Importantly, L-mCherry fluorescence in transfected cells was readily detectable and distributed in a punctate pattern characteristic for inclusion bodies. A recombinant ebolavirus encoding L-mCherry instead of L was rescued and showed virtually identical growth kinetics and endpoint titers to those for wild-type virus. Using this virus, we showed that the onset of inclusion body formation corresponds to the onset of viral genome replication, but that viral transcription occurs prior to inclusion body formation. Live-cell imaging further showed that inclusion bodies are highly dynamic structures and that they can undergo dramatic reorganization during cell division. Finally, by labeling nascent RNAs using click technology we showed that inclusion bodies are indeed the site of viral RNA synthesis. Based on these data we conclude that, rather than being inert aggregates of nucleocapsids, ebolavirus inclusion bodies are in fact complex and dynamic structures and an important site at which viral RNA replication takes place.
AbstractList	Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological significance has been debated, and they have been suggested to be aggregates of viral proteins without any further biological function. However, recent data for other viruses that produce similar structures have suggested that inclusion bodies might be involved in genome replication and transcription. In order to study filovirus inclusion bodies, we fused mCherry to the ebolavirus polymerase L, which is found in inclusion bodies. The resulting L-mCherry fusion protein was functional in minigenome assays and incorporated into virus-like particles. Importantly, L-mCherry fluorescence in transfected cells was readily detectable and distributed in a punctate pattern characteristic for inclusion bodies. A recombinant ebolavirus encoding L-mCherry instead of L was rescued and showed virtually identical growth kinetics and endpoint titers to those for wild-type virus. Using this virus, we showed that the onset of inclusion body formation corresponds to the onset of viral genome replication, but that viral transcription occurs prior to inclusion body formation. Live-cell imaging further showed that inclusion bodies are highly dynamic structures and that they can undergo dramatic reorganization during cell division. Finally, by labeling nascent RNAs using click technology we showed that inclusion bodies are indeed the site of viral RNA synthesis. Based on these data we conclude that, rather than being inert aggregates of nucleocapsids, ebolavirus inclusion bodies are in fact complex and dynamic structures and an important site at which viral RNA replication takes place. Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological significance has been debated, and they have been suggested to be aggregates of viral proteins without any further biological function. However, recent data for other viruses that produce similar structures have suggested that inclusion bodies might be involved in genome replication and transcription. In order to study filovirus inclusion bodies, we fused mCherry to the ebolavirus polymerase L, which is found in inclusion bodies. The resulting L-mCherry fusion protein was functional in minigenome assays and incorporated into virus-like particles. Importantly, L-mCherry fluorescence in transfected cells was readily detectable and distributed in a punctate pattern characteristic for inclusion bodies. A recombinant ebolavirus encoding L-mCherry instead of L was rescued and showed virtually identical growth kinetics and endpoint titers to those for wild-type virus. Using this virus, we showed that the onset of inclusion body formation corresponds to the onset of viral genome replication, but that viral transcription occurs prior to inclusion body formation. Live-cell imaging further showed that inclusion bodies are highly dynamic structures and that they can undergo dramatic reorganization during cell division. Finally, by labeling nascent RNAs using click technology we showed that inclusion bodies are indeed the site of viral RNA synthesis. Based on these data we conclude that, rather than being inert aggregates of nucleocapsids, ebolavirus inclusion bodies are in fact complex and dynamic structures and an important site at which viral RNA replication takes place.Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological significance has been debated, and they have been suggested to be aggregates of viral proteins without any further biological function. However, recent data for other viruses that produce similar structures have suggested that inclusion bodies might be involved in genome replication and transcription. In order to study filovirus inclusion bodies, we fused mCherry to the ebolavirus polymerase L, which is found in inclusion bodies. The resulting L-mCherry fusion protein was functional in minigenome assays and incorporated into virus-like particles. Importantly, L-mCherry fluorescence in transfected cells was readily detectable and distributed in a punctate pattern characteristic for inclusion bodies. A recombinant ebolavirus encoding L-mCherry instead of L was rescued and showed virtually identical growth kinetics and endpoint titers to those for wild-type virus. Using this virus, we showed that the onset of inclusion body formation corresponds to the onset of viral genome replication, but that viral transcription occurs prior to inclusion body formation. Live-cell imaging further showed that inclusion bodies are highly dynamic structures and that they can undergo dramatic reorganization during cell division. Finally, by labeling nascent RNAs using click technology we showed that inclusion bodies are indeed the site of viral RNA synthesis. Based on these data we conclude that, rather than being inert aggregates of nucleocapsids, ebolavirus inclusion bodies are in fact complex and dynamic structures and an important site at which viral RNA replication takes place.
Author	Feldmann, Heinz Groseth, Allison Schudt, Gordian Herwig, Astrid Weber, Michaela Dolnik, Olga Basler, Christopher F Shabman, Reed S Hoenen, Thomas Becker, Stephan
Author_xml	– sequence: 1 givenname: Thomas surname: Hoenen fullname: Hoenen, Thomas organization: Laboratory for Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA – sequence: 2 givenname: Reed S surname: Shabman fullname: Shabman, Reed S – sequence: 3 givenname: Allison surname: Groseth fullname: Groseth, Allison – sequence: 4 givenname: Astrid surname: Herwig fullname: Herwig, Astrid – sequence: 5 givenname: Michaela surname: Weber fullname: Weber, Michaela – sequence: 6 givenname: Gordian surname: Schudt fullname: Schudt, Gordian – sequence: 7 givenname: Olga surname: Dolnik fullname: Dolnik, Olga – sequence: 8 givenname: Christopher F surname: Basler fullname: Basler, Christopher F – sequence: 9 givenname: Stephan surname: Becker fullname: Becker, Stephan – sequence: 10 givenname: Heinz surname: Feldmann fullname: Feldmann, Heinz
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Snippet	Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological...
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SubjectTerms	Animals Artificial Gene Fusion Cell Line Ebolavirus - physiology Genes, Reporter Humans Inclusion Bodies, Viral - virology Luminescent Proteins - analysis Luminescent Proteins - genetics Microscopy, Fluorescence Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics Red Fluorescent Protein RNA-Dependent RNA Polymerase - genetics Transfection Viral Proteins - genetics Virus Replication
Title	Inclusion bodies are a site of ebolavirus replication
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