Optimization of polysaccharides extraction from quince peels: partial characterization, antioxidant and antiproliferative properties

In this study, Box-Behnken Design was used to optimize the ultrasonic extraction of polysaccharides from quince peels (QPPs) by ascorbic acid and the effect of extraction temperature, extraction time and pH was evaluated. Under optimized conditions of temperature 90 °C, 60 min sonication time and pH...

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Vydané v:Natural product research Ročník 34; číslo 10; s. 1470 - 1474
Hlavní autori: Dammak, Mariem Itaimi, Mzoughi, Zeineb, Chakroun, Ibtissem, Mansour, Hedi Ben, Le Cerf, Didier, Majdoub, Hatem
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England Taylor & Francis 18.05.2020
Taylor & Francis Ltd
Predmet:
2,2-diphenyl-1-picrylhydrazyl
Anticancer properties
antineoplastic agents
antioxidant activity
antioxidant and antiproliferative activities
Antioxidants
Antiproliferatives
Arabinose
Ascorbic acid
Cell lines
Chemical Sciences
Design optimization
dose response
experimental design
Food industry
Fourier analysis
Fourier transform infrared spectroscopy
Fourier transforms
functional foods
Functional foods & nutraceuticals
Galactose
galacturonic acid
gel chromatography
glucose
human cell lines
industry
Infrared spectroscopy
Mannose
Material chemistry
mice
Monosaccharides
NMR
Nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
pH effects
physico-chemical characterization
Polymers
Polysaccharides
Quince
quinces
Saccharides
Size exclusion chromatography
Sonication
temperature
ultrasonics
ultrasound-assisted extraction
viability
xylose
polysaccharides
Quince
ascorbic acid
ultrasound-assisted extraction
antioxidant and antiproliferative activities
physico-chemical characterization
ISSN:1478-6419, 1478-6427, 1478-6427, 1029-2349
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Shrnutí:In this study, Box-Behnken Design was used to optimize the ultrasonic extraction of polysaccharides from quince peels (QPPs) by ascorbic acid and the effect of extraction temperature, extraction time and pH was evaluated. Under optimized conditions of temperature 90 °C, 60 min sonication time and pH = 3.26, the extraction yield, the galacturonic acid yield and the concentration of sample required to scavenge 50% of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) values of QPPs were respectively 10.25%, 3.86% and 1.35 mg/mL. The QPPs extracted under optimum conditions was characterized by Fourier transform infrared spectroscopy (FTIR), Nuclear magnetic resonance ( 1  H NMR) and Size exclusion chromatography (SEC/MALS/VD/DRI). The monosaccharide analysis revealed that arabinose was the most abundant, followed by galactose, glucose, mannose and xylose. Moreover, QPPs showed significant antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric- reducing antioxidant power (FRAP)) and reduced viability of human Caco-2 and murine B-16 cell lines in a dose-dependent manner. Hence QPPs could be used as antitumor agent in functional foods andpharmaceutical industries.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1478-6419
1478-6427
1478-6427
1029-2349
DOI:10.1080/14786419.2018.1514403