Characterization of Immune Responses Induced by Ebola Virus Glycoprotein (GP) and Truncated GP Isoform DNA Vaccines and Protection Against Lethal Ebola Virus Challenge in Mice

In addition to its surface glycoprotein (GP), Ebola virus directs the production of large quantities of a truncated glycoprotein isoform (sGP) that is secreted into the extracellular space. We recently reported that sGP actively diverts host antibody responses against the epitopes that it shares wit...

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Veröffentlicht in:	The Journal of infectious diseases Jg. 212 Suppl 2; S. S398
Hauptverfasser:	Li, Wenfang, Ye, Ling, Carrion, Jr, Ricardo, Mohan, Gopi S, Nunneley, Jerritt, Staples, Hilary, Ticer, Anysha, Patterson, Jean L, Compans, Richard W, Yang, Chinglai
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.10.2015
Schlagworte:	Animals Antibodies, Viral - immunology Antibody Formation - immunology Ebola Vaccines - immunology Ebolavirus - immunology Female Glycoproteins - immunology HEK293 Cells Hemorrhagic Fever, Ebola - immunology Hemorrhagic Fever, Ebola - virology Humans Immunization, Secondary - methods Mice Mice, Inbred BALB C Protein Isoforms - immunology Vaccination - methods Vaccines, DNA - immunology Viral Proteins - immunology antigenic subversion vaccine Ebola soluble GP
ISSN:	1537-6613, 1537-6613
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Abstract	In addition to its surface glycoprotein (GP), Ebola virus directs the production of large quantities of a truncated glycoprotein isoform (sGP) that is secreted into the extracellular space. We recently reported that sGP actively diverts host antibody responses against the epitopes that it shares with GP and thereby allows itself to absorb anti-GP antibodies, a phenomenon we termed "antigenic subversion." To investigate the effect of antigenic subversion by sGP on protection against virus infection, we compared immune responses induced by different prime-boost immunization regimens with GP and sGP DNA vaccines in mice and their efficacy against lethal Ebola virus challenge. Similar levels of anti-GP antibodies were induced by 2 immunizations with sGP and GP DNA vaccines. However, 2 immunizations with GP but not sGP DNA vaccine fully protected mice from lethal challenge. Boosting with sGP or GP DNA vaccine in mice that had been primed by GP or sGP DNA vaccine augmented the levels of anti-GP antibody responses and further improved protective efficacy against Ebola virus infection. These results show that both the quality and the levels of anti-GP antibody responses affect the efficacy of protection against Ebola virus infection.
AbstractList	In addition to its surface glycoprotein (GP), Ebola virus directs the production of large quantities of a truncated glycoprotein isoform (sGP) that is secreted into the extracellular space. We recently reported that sGP actively diverts host antibody responses against the epitopes that it shares with GP and thereby allows itself to absorb anti-GP antibodies, a phenomenon we termed "antigenic subversion." To investigate the effect of antigenic subversion by sGP on protection against virus infection, we compared immune responses induced by different prime-boost immunization regimens with GP and sGP DNA vaccines in mice and their efficacy against lethal Ebola virus challenge. Similar levels of anti-GP antibodies were induced by 2 immunizations with sGP and GP DNA vaccines. However, 2 immunizations with GP but not sGP DNA vaccine fully protected mice from lethal challenge. Boosting with sGP or GP DNA vaccine in mice that had been primed by GP or sGP DNA vaccine augmented the levels of anti-GP antibody responses and further improved protective efficacy against Ebola virus infection. These results show that both the quality and the levels of anti-GP antibody responses affect the efficacy of protection against Ebola virus infection.In addition to its surface glycoprotein (GP), Ebola virus directs the production of large quantities of a truncated glycoprotein isoform (sGP) that is secreted into the extracellular space. We recently reported that sGP actively diverts host antibody responses against the epitopes that it shares with GP and thereby allows itself to absorb anti-GP antibodies, a phenomenon we termed "antigenic subversion." To investigate the effect of antigenic subversion by sGP on protection against virus infection, we compared immune responses induced by different prime-boost immunization regimens with GP and sGP DNA vaccines in mice and their efficacy against lethal Ebola virus challenge. Similar levels of anti-GP antibodies were induced by 2 immunizations with sGP and GP DNA vaccines. However, 2 immunizations with GP but not sGP DNA vaccine fully protected mice from lethal challenge. Boosting with sGP or GP DNA vaccine in mice that had been primed by GP or sGP DNA vaccine augmented the levels of anti-GP antibody responses and further improved protective efficacy against Ebola virus infection. These results show that both the quality and the levels of anti-GP antibody responses affect the efficacy of protection against Ebola virus infection. In addition to its surface glycoprotein (GP), Ebola virus directs the production of large quantities of a truncated glycoprotein isoform (sGP) that is secreted into the extracellular space. We recently reported that sGP actively diverts host antibody responses against the epitopes that it shares with GP and thereby allows itself to absorb anti-GP antibodies, a phenomenon we termed "antigenic subversion." To investigate the effect of antigenic subversion by sGP on protection against virus infection, we compared immune responses induced by different prime-boost immunization regimens with GP and sGP DNA vaccines in mice and their efficacy against lethal Ebola virus challenge. Similar levels of anti-GP antibodies were induced by 2 immunizations with sGP and GP DNA vaccines. However, 2 immunizations with GP but not sGP DNA vaccine fully protected mice from lethal challenge. Boosting with sGP or GP DNA vaccine in mice that had been primed by GP or sGP DNA vaccine augmented the levels of anti-GP antibody responses and further improved protective efficacy against Ebola virus infection. These results show that both the quality and the levels of anti-GP antibody responses affect the efficacy of protection against Ebola virus infection.
Author	Compans, Richard W Li, Wenfang Patterson, Jean L Ticer, Anysha Yang, Chinglai Carrion, Jr, Ricardo Nunneley, Jerritt Ye, Ling Mohan, Gopi S Staples, Hilary
Author_xml	– sequence: 1 givenname: Wenfang surname: Li fullname: Li, Wenfang organization: Department of Microbiology and Immunology Emory Vaccine Center, Emory University, Atlanta, Georgia – sequence: 2 givenname: Ling surname: Ye fullname: Ye, Ling organization: Department of Microbiology and Immunology Emory Vaccine Center, Emory University, Atlanta, Georgia – sequence: 3 givenname: Ricardo surname: Carrion, Jr fullname: Carrion, Jr, Ricardo organization: Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio – sequence: 4 givenname: Gopi S surname: Mohan fullname: Mohan, Gopi S organization: Department of Microbiology and Immunology Emory Vaccine Center, Emory University, Atlanta, Georgia – sequence: 5 givenname: Jerritt surname: Nunneley fullname: Nunneley, Jerritt organization: Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio – sequence: 6 givenname: Hilary surname: Staples fullname: Staples, Hilary organization: Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio – sequence: 7 givenname: Anysha surname: Ticer fullname: Ticer, Anysha organization: Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio – sequence: 8 givenname: Jean L surname: Patterson fullname: Patterson, Jean L organization: Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio – sequence: 9 givenname: Richard W surname: Compans fullname: Compans, Richard W organization: Department of Microbiology and Immunology Emory Vaccine Center, Emory University, Atlanta, Georgia – sequence: 10 givenname: Chinglai surname: Yang fullname: Yang, Chinglai organization: Department of Microbiology and Immunology Emory Vaccine Center, Emory University, Atlanta, Georgia
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References	23271969 - PLoS Pathog. 2012;8(12):e1003065 23408633 - J Virol. 2013 May;87(9):4952-64 23737747 - PLoS Pathog. 2013;9(5):e1003389 9427604 - Nat Med. 1998 Jan;4(1):37-42 16678231 - Virology. 2006 Aug 1;351(2):260-70 17589545 - Nat Rev Immunol. 2007 Jul;7(7):556-67 18615077 - Nature. 2008 Jul 10;454(7201):177-82 23319647 - Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1893-8 12646921 - Nature. 2003 Mar 20;422(6929):307-12 17940980 - J Infect Dis. 2007 Nov 15;196 Suppl 2:S430-7 19369954 - Nat Rev Microbiol. 2009 May;7(5):393-400 17428868 - J Virol. 2007 Jun;81(12):6379-88 18986663 - Virology. 2009 Jan 5;383(1):12-21 16683867 - PLoS Med. 2006 Jun;3(6):e177 23155478 - Sci Rep. 2012;2:811 15288821 - Lancet Infect Dis. 2004 Aug;4(8):487-98 25370495 - MBio. 2014;5(6):e02011 16988008 - Clin Vaccine Immunol. 2006 Nov;13(11):1267-77 9614872 - Virology. 1998 May 25;245(1):110-9 19023410 - PLoS Pathog. 2008 Nov;4(11):e1000212 8553543 - Virology. 1995 Dec 20;214(2):421-30 22700957 - Sci Transl Med. 2012 Jun 13;4(138):138ra81 17698361 - Trends Microbiol. 2007 Sep;15(9):408-16 19010509 - Virology. 2009 Jan 20;383(2):348-61 8622982 - Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3602-7 10470276 - Arch Virol Suppl. 1999;15:159-69 14673108 - Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15889-94 18216185 - Clin Vaccine Immunol. 2008 Mar;15(3):460-7 22411795 - Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5034-9 15937495 - Nat Med. 2005 Jul;11(7):786-90 23115355 - Sci Transl Med. 2012 Oct 31;4(158):158ra146 21987773 - J Infect Dis. 2011 Nov;204 Suppl 3:S941-6 17241710 - Vaccine. 2007 Mar 1;25(11):1923-34
References_xml	– reference: 23737747 - PLoS Pathog. 2013;9(5):e1003389 – reference: 17698361 - Trends Microbiol. 2007 Sep;15(9):408-16 – reference: 22411795 - Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5034-9 – reference: 8622982 - Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3602-7 – reference: 23271969 - PLoS Pathog. 2012;8(12):e1003065 – reference: 19010509 - Virology. 2009 Jan 20;383(2):348-61 – reference: 10470276 - Arch Virol Suppl. 1999;15:159-69 – reference: 17589545 - Nat Rev Immunol. 2007 Jul;7(7):556-67 – reference: 18615077 - Nature. 2008 Jul 10;454(7201):177-82 – reference: 9427604 - Nat Med. 1998 Jan;4(1):37-42 – reference: 9614872 - Virology. 1998 May 25;245(1):110-9 – reference: 19369954 - Nat Rev Microbiol. 2009 May;7(5):393-400 – reference: 23319647 - Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1893-8 – reference: 23155478 - Sci Rep. 2012;2:811 – reference: 16678231 - Virology. 2006 Aug 1;351(2):260-70 – reference: 16683867 - PLoS Med. 2006 Jun;3(6):e177 – reference: 17428868 - J Virol. 2007 Jun;81(12):6379-88 – reference: 12646921 - Nature. 2003 Mar 20;422(6929):307-12 – reference: 19023410 - PLoS Pathog. 2008 Nov;4(11):e1000212 – reference: 14673108 - Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15889-94 – reference: 23408633 - J Virol. 2013 May;87(9):4952-64 – reference: 22700957 - Sci Transl Med. 2012 Jun 13;4(138):138ra81 – reference: 21987773 - J Infect Dis. 2011 Nov;204 Suppl 3:S941-6 – reference: 8553543 - Virology. 1995 Dec 20;214(2):421-30 – reference: 17940980 - J Infect Dis. 2007 Nov 15;196 Suppl 2:S430-7 – reference: 15288821 - Lancet Infect Dis. 2004 Aug;4(8):487-98 – reference: 18216185 - Clin Vaccine Immunol. 2008 Mar;15(3):460-7 – reference: 25370495 - MBio. 2014;5(6):e02011 – reference: 16988008 - Clin Vaccine Immunol. 2006 Nov;13(11):1267-77 – reference: 18986663 - Virology. 2009 Jan 5;383(1):12-21 – reference: 17241710 - Vaccine. 2007 Mar 1;25(11):1923-34 – reference: 15937495 - Nat Med. 2005 Jul;11(7):786-90 – reference: 23115355 - Sci Transl Med. 2012 Oct 31;4(158):158ra146
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SubjectTerms	Animals Antibodies, Viral - immunology Antibody Formation - immunology Ebola Vaccines - immunology Ebolavirus - immunology Female Glycoproteins - immunology HEK293 Cells Hemorrhagic Fever, Ebola - immunology Hemorrhagic Fever, Ebola - virology Humans Immunization, Secondary - methods Mice Mice, Inbred BALB C Protein Isoforms - immunology Vaccination - methods Vaccines, DNA - immunology Viral Proteins - immunology
Title	Characterization of Immune Responses Induced by Ebola Virus Glycoprotein (GP) and Truncated GP Isoform DNA Vaccines and Protection Against Lethal Ebola Virus Challenge in Mice
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