Biases for detecting arbuscular mycorrhizal fungal mixture by terminal restriction fragment length polymorphism (T-RFLP)

Terminal restriction fragment length polymorphism (T-RFLP) analysis of amplified ribosomal RNA genes is used for profiling microbial communities and sometimes for species richness and relative abundance estimation in environmental samples. However, the T-RFLP fingerprint may be subject to biases dur...

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Bibliographic Details
Published in:	World journal of microbiology & biotechnology Vol. 30; no. 1; pp. 77 - 86
Main Authors:	Watanarojanaporn, N., Longtonglang, A., Boonkerd, N., Tittabutr, P., Lee, J., Teaumroong, N.
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01.01.2014 Springer Nature B.V
Subjects:	Abundance Amplification Analysis Applied Microbiology Bias Bias (Epidemiology) Biochemistry Biomedical and Life Sciences Biotechnology Chloroform classification clones Cloning Deoxyribonucleic acid Diagnostic Errors DNA Environmental Engineering/Biotechnology Flowers & plants Fungi Genes genetics Genomics Life Sciences methods Microbial activity microbial communities Microbiology Microorganisms Molecular Typing Molecular Typing - methods Mycological Typing Techniques Mycological Typing Techniques - methods Mycorrhizae Mycorrhizae - classification Mycorrhizae - genetics mycorrhizal fungi Original Paper Phenols Plasmids Polymerase chain reaction Polymorphism Polymorphism, Restriction Fragment Length Purification purification methods Relative abundance restriction fragment length polymorphism ribosomal RNA RNA, Ribosomal, 18S RNA, Ribosomal, 18S - genetics species diversity Species richness Studies Terminals vesicular arbuscular mycorrhizae T-RFs peak area Plant genomic DNA AMF plasmid DNA T-RFLP Pairwise mixes
ISSN:	0959-3993, 1573-0972, 1573-0972
Online Access:	Get full text
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