Use of Matrigel in culture affects cell phenotype and gene expression in the first trimester trophoblast cell line HTR8/SVneo

There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture...

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Veröffentlicht in:	Placenta (Eastbourne) Jg. 33; H. 7; S. 586 - 588
Hauptverfasser:	Highet, A.R., Zhang, V.J., Heinemann, G.K., Roberts, C.T.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Kidlington Elsevier Ltd 01.07.2012 Elsevier
Schlagworte:	Biological and medical sciences Cell Differentiation - genetics Cell Line Collagen - administration & dosage Collagen - pharmacology Drug Combinations Embryology: invertebrates and vertebrates. Teratology Endothelial Cells - physiology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - drug effects Gestational Age Humans Integrin alpha1 - genetics Internal Medicine Keratins - genetics Laminin - administration & dosage Laminin - pharmacology Matrigel Matrix Metalloproteinase 2 - genetics Obstetrics and Gynecology Phenotype Plastics Proteoglycans - administration & dosage Proteoglycans - pharmacology Trophoblast Trophoblasts - metabolism Gene expression Trophoblast Matrigel Pregnancy Trophoblaste Cell culture Vertebrata Phenotype Cell line Mammalia Fetal membrane First trimester In vitro
ISSN:	0143-4004, 1532-3102, 1532-3102
Online-Zugang:	Volltext
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