On the Determination of Uncertainty and Limit of Detection in Label-Free Biosensors
A significant amount of noteworthy articles reviewing different label-free biosensors are being published in the last years. Most of the times, the comparison among the different biosensors is limited by the procedure used of calculating the limit of detection and the measurement uncertainty. This a...
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| Vydané v: | Sensors (Basel, Switzerland) Ročník 18; číslo 7; s. 2038 |
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| Hlavní autori: | , , , , , , , , |
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| Jazyk: | English |
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26.06.2018
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| Abstract | A significant amount of noteworthy articles reviewing different label-free biosensors are being published in the last years. Most of the times, the comparison among the different biosensors is limited by the procedure used of calculating the limit of detection and the measurement uncertainty. This article clarifies and establishes a simple procedure to determine the calibration function and the uncertainty of the concentration measured at any point of the measuring interval of a generic label-free biosensor. The value of the limit of detection arises naturally from this model as the limit at which uncertainty tends when the concentration tends to zero. The need to provide additional information, such as the measurement interval and its linearity, among others, on the analytical systems and biosensor in addition to the detection limit is pointed out. Finally, the model is applied to curves that are typically obtained in immunoassays and a discussion is made on the application validity of the model and its limitations. |
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| AbstractList | A significant amount of noteworthy articles reviewing different label-free biosensors are being published in the last years. Most of the times, the comparison among the different biosensors is limited by the procedure used of calculating the limit of detection and the measurement uncertainty. This article clarifies and establishes a simple procedure to determine the calibration function and the uncertainty of the concentration measured at any point of the measuring interval of a generic label-free biosensor. The value of the limit of detection arises naturally from this model as the limit at which uncertainty tends when the concentration tends to zero. The need to provide additional information, such as the measurement interval and its linearity, among others, on the analytical systems and biosensor in addition to the detection limit is pointed out. Finally, the model is applied to curves that are typically obtained in immunoassays and a discussion is made on the application validity of the model and its limitations. A significant amount of noteworthy articles reviewing different label-free biosensors are being published in the last years. Most of the times, the comparison among the different biosensors is limited by the procedure used of calculating the limit of detection and the measurement uncertainty. This article clarifies and establishes a simple procedure to determine the calibration function and the uncertainty of the concentration measured at any point of the measuring interval of a generic label-free biosensor. The value of the limit of detection arises naturally from this model as the limit at which uncertainty tends when the concentration tends to zero. The need to provide additional information, such as the measurement interval and its linearity, among others, on the analytical systems and biosensor in addition to the detection limit is pointed out. Finally, the model is applied to curves that are typically obtained in immunoassays and a discussion is made on the application validity of the model and its limitations.A significant amount of noteworthy articles reviewing different label-free biosensors are being published in the last years. Most of the times, the comparison among the different biosensors is limited by the procedure used of calculating the limit of detection and the measurement uncertainty. This article clarifies and establishes a simple procedure to determine the calibration function and the uncertainty of the concentration measured at any point of the measuring interval of a generic label-free biosensor. The value of the limit of detection arises naturally from this model as the limit at which uncertainty tends when the concentration tends to zero. The need to provide additional information, such as the measurement interval and its linearity, among others, on the analytical systems and biosensor in addition to the detection limit is pointed out. Finally, the model is applied to curves that are typically obtained in immunoassays and a discussion is made on the application validity of the model and its limitations. |
| Author | Laguna, María F. Santamaría, Beatriz Holgado, Miguel Maigler, María Victoria Lavín, Álvaro Vicente, Jesús de Hernández, Ana L. Casquel, Rafael Ramírez, Yolanda |
| AuthorAffiliation | 4 BioOptical Detection, Centro de Tecnología Biomédica, Campus Montegancedo, 28223 Madrid, Spain 3 Laboratorio de Metrología y Metrotecnia, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/ José Gutiérrez Abascal, 2, 28006 Madrid, Spain 1 Centre for Biomedical Technology, Optics, Photonics and Biophotonics Laboratory, Campus Montegancedo, Universidad Politécnica de Madrid, 28223 Madrid, Spain; alvaro.lavin@upm.es (A.L.); mariafe.laguna@upm.es (M.F.L.); rafael.casquel@upm.es (R.C.); beatriz.santamaria@ctb.upm.es (B.S.); m.maigler@biod.es (M.V.M.); ana.lopez@upm.es (A.L.H.); y.ramirez@biod.es (Y.R.) 2 Department of Applied Physics, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/José Gutiérrez Abascal, 2, 28006 Madrid, Spain; jvo@etsii.upm.es |
| AuthorAffiliation_xml | – name: 2 Department of Applied Physics, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/José Gutiérrez Abascal, 2, 28006 Madrid, Spain; jvo@etsii.upm.es – name: 1 Centre for Biomedical Technology, Optics, Photonics and Biophotonics Laboratory, Campus Montegancedo, Universidad Politécnica de Madrid, 28223 Madrid, Spain; alvaro.lavin@upm.es (A.L.); mariafe.laguna@upm.es (M.F.L.); rafael.casquel@upm.es (R.C.); beatriz.santamaria@ctb.upm.es (B.S.); m.maigler@biod.es (M.V.M.); ana.lopez@upm.es (A.L.H.); y.ramirez@biod.es (Y.R.) – name: 4 BioOptical Detection, Centro de Tecnología Biomédica, Campus Montegancedo, 28223 Madrid, Spain – name: 3 Laboratorio de Metrología y Metrotecnia, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/ José Gutiérrez Abascal, 2, 28006 Madrid, Spain |
| Author_xml | – sequence: 1 givenname: Álvaro surname: Lavín fullname: Lavín, Álvaro – sequence: 2 givenname: Jesús de surname: Vicente fullname: Vicente, Jesús de – sequence: 3 givenname: Miguel surname: Holgado fullname: Holgado, Miguel – sequence: 4 givenname: María F. orcidid: 0000-0002-1580-4509 surname: Laguna fullname: Laguna, María F. – sequence: 5 givenname: Rafael surname: Casquel fullname: Casquel, Rafael – sequence: 6 givenname: Beatriz orcidid: 0000-0003-3714-7922 surname: Santamaría fullname: Santamaría, Beatriz – sequence: 7 givenname: María Victoria surname: Maigler fullname: Maigler, María Victoria – sequence: 8 givenname: Ana L. surname: Hernández fullname: Hernández, Ana L. – sequence: 9 givenname: Yolanda surname: Ramírez fullname: Ramírez, Yolanda |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29949904$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Biosensing Techniques - standards calculation of the limit of detection Calibration label-free biosensing performance Limit of Detection limit of quantification measurement uncertainty Reproducibility of Results Review Uncertainty |
| Title | On the Determination of Uncertainty and Limit of Detection in Label-Free Biosensors |
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