Adipose tissue-derived ECM hydrogels and their use as 3D culture scaffold
Adipose tissue has the therapeutic capacity in the form of a fat graft, for example, for treatment of irradiation-induced scars and difficult to heal dermal wounds. For large-scale clinical application, an off-the-shelf product is warranted. In recent years, ECM-derived hydrogels are postulated to h...
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| Vydáno v: | Artificial cells, nanomedicine, and biotechnology Ročník 47; číslo 1; s. 1693 - 1701 |
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| Médium: | Journal Article |
| Jazyk: | angličtina |
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Taylor & Francis
01.12.2019
Taylor & Francis Ltd Taylor & Francis Group |
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| ISSN: | 2169-1401, 2169-141X, 2169-141X |
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| Abstract | Adipose tissue has the therapeutic capacity in the form of a fat graft, for example, for treatment of irradiation-induced scars and difficult to heal dermal wounds. For large-scale clinical application, an off-the-shelf product is warranted. In recent years, ECM-derived hydrogels are postulated to harbour therapeutic capacity and might even replicate the beneficial effects of adipose tissue. In normal homeostasis, the natural ECM acts as a deposit of growth factors, that releases them over time. In the healing of lesions, this might promote cell accumulation and proliferation which in turn stimulates angiogenesis and repair. The decellularization of tissue and the generation of hydrogels may leave cytotoxic traces. Therefore, our research assessed the cytotoxic effect of human adipose tissue-derived ECM hydrogels on connective tissue cells i.e. fibroblasts. The results showed no cytotoxicity, meaning the hydrogels caused no cell death. Cell migration and survival were observed when cultured in ECM hydrogels and followed for 7 days. Cell survival in the hydrogel was confirmed with CFDA staining and also cells showed the ability to penetrate and migrate throughout the gel. We conclude that ECM hydrogels are promising to use as innovative therapy for wound healing. |
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| AbstractList | Adipose tissue has the therapeutic capacity in the form of a fat graft, for example, for treatment of irradiation-induced scars and difficult to heal dermal wounds. For large-scale clinical application, an off-the-shelf product is warranted. In recent years, ECM-derived hydrogels are postulated to harbour therapeutic capacity and might even replicate the beneficial effects of adipose tissue. In normal homeostasis, the natural ECM acts as a deposit of growth factors, that releases them over time. In the healing of lesions, this might promote cell accumulation and proliferation which in turn stimulates angiogenesis and repair. The decellularization of tissue and the generation of hydrogels may leave cytotoxic traces. Therefore, our research assessed the cytotoxic effect of human adipose tissue-derived ECM hydrogels on connective tissue cells i.e. fibroblasts. The results showed no cytotoxicity, meaning the hydrogels caused no cell death. Cell migration and survival were observed when cultured in ECM hydrogels and followed for 7 days. Cell survival in the hydrogel was confirmed with CFDA staining and also cells showed the ability to penetrate and migrate throughout the gel. We conclude that ECM hydrogels are promising to use as innovative therapy for wound healing.Adipose tissue has the therapeutic capacity in the form of a fat graft, for example, for treatment of irradiation-induced scars and difficult to heal dermal wounds. For large-scale clinical application, an off-the-shelf product is warranted. In recent years, ECM-derived hydrogels are postulated to harbour therapeutic capacity and might even replicate the beneficial effects of adipose tissue. In normal homeostasis, the natural ECM acts as a deposit of growth factors, that releases them over time. In the healing of lesions, this might promote cell accumulation and proliferation which in turn stimulates angiogenesis and repair. The decellularization of tissue and the generation of hydrogels may leave cytotoxic traces. Therefore, our research assessed the cytotoxic effect of human adipose tissue-derived ECM hydrogels on connective tissue cells i.e. fibroblasts. The results showed no cytotoxicity, meaning the hydrogels caused no cell death. Cell migration and survival were observed when cultured in ECM hydrogels and followed for 7 days. Cell survival in the hydrogel was confirmed with CFDA staining and also cells showed the ability to penetrate and migrate throughout the gel. We conclude that ECM hydrogels are promising to use as innovative therapy for wound healing. Adipose tissue has the therapeutic capacity in the form of a fat graft, for example, for treatment of irradiation-induced scars and difficult to heal dermal wounds. For large-scale clinical application, an off-the-shelf product is warranted. In recent years, ECM-derived hydrogels are postulated to harbour therapeutic capacity and might even replicate the beneficial effects of adipose tissue. In normal homeostasis, the natural ECM acts as a deposit of growth factors, that releases them over time. In the healing of lesions, this might promote cell accumulation and proliferation which in turn stimulates angiogenesis and repair. The decellularization of tissue and the generation of hydrogels may leave cytotoxic traces. Therefore, our research assessed the cytotoxic effect of human adipose tissue-derived ECM hydrogels on connective tissue cells i.e. fibroblasts. The results showed no cytotoxicity, meaning the hydrogels caused no cell death. Cell migration and survival were observed when cultured in ECM hydrogels and followed for 7 days. Cell survival in the hydrogel was confirmed with CFDA staining and also cells showed the ability to penetrate and migrate throughout the gel. We conclude that ECM hydrogels are promising to use as innovative therapy for wound healing. |
| Author | Harmsen, Martin C. van Dongen, Joris A. Brouwer, Linda A. Getova, Vasilena E. |
| Author_xml | – sequence: 1 givenname: Vasilena E. surname: Getova fullname: Getova, Vasilena E. organization: Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen – sequence: 2 givenname: Joris A. surname: van Dongen fullname: van Dongen, Joris A. organization: Department of Plastic Surgery, University Medical Center Groningen, University of Groningen – sequence: 3 givenname: Linda A. surname: Brouwer fullname: Brouwer, Linda A. organization: Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen – sequence: 4 givenname: Martin C. surname: Harmsen fullname: Harmsen, Martin C. organization: Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31062610$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1097/00001721-200001000-00003 10.1002/jbm.a.35726 10.1111/wrr.12482 10.1016/j.bjps.2011.03.031 10.1155/2017/5237063 10.1002/stem.2726 10.1016/j.cjtee.2014.10.005 10.1007/s00795-017-0158-7 10.1186/s12929-017-0318-z 10.1002/jbm.a.35844 10.1016/j.biomaterials.2016.03.014 10.1016/j.biomaterials.2009.06.045 10.4103/2319-4170.132899 10.1186/s13287-017-0598-y 10.1126/scitranslmed.3005503 10.1089/wound.2015.0652 10.1016/j.ymeth.2008.03.006 10.1177/1090820X12455658 10.1111/j.1476-5381.2012.01965.x 10.5966/sctm.2014-0161 10.1016/j.actbio.2017.12.019 10.1242/jcs.023820 10.1088/1748-605X/aa6a63 10.1155/2017/9747010 10.1016/j.jconrel.2016.07.045 10.1016/j.actbio.2012.11.003 10.1097/PRS.0000000000002891 10.1053/ejvs.1998.0677 10.1371/journal.pone.0076373 10.1007/s00125-018-4713-0 |
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| SubjectTerms | Adipose tissue adipose tissue-derived stromal cells (ASC) Angiogenesis Cell adhesion & migration Cell culture Cell death Cell migration Cell survival Connective tissues Cytotoxicity Extracellular matrix Extracellular matrix (ECM) Fibroblasts Growth factors Homeostasis hydrogel Hydrogels Radiation Reconstructive surgery Scars Survival Tissue culture Toxicity Wound healing |
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