Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9

The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets bearing a PAM (protospacer adjacent motif) and complementarity to the guide RNA. A recent study showed that, whereas wild-type Streptococcus pyogenes Cas9 (SpCas9) recognizes the 5'-NGG-3' PAM, the engineered VQR, EQR, an...

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Bibliographic Details

Published in:	Molecular cell Vol. 61; no. 6; p. 886
Main Authors:	Hirano, Seiichi, Nishimasu, Hiroshi, Ishitani, Ryuichiro, Nureki, Osamu
Format:	Journal Article
Language:	English
Published:	United States 17.03.2016
Subjects:	Bacterial Proteins - chemistry Bacterial Proteins - genetics CRISPR-Associated Protein 9 CRISPR-Cas Systems Crystallography, X-Ray DNA - chemistry DNA - genetics DNA, Intergenic - genetics Endonucleases - chemistry Endonucleases - genetics Genetic Engineering Mutation RNA, Guide, CRISPR-Cas Systems - chemistry RNA, Guide, CRISPR-Cas Systems - genetics Substrate Specificity
ISSN:	1097-4164, 1097-4164
Online Access:	Get more information
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