Sertoli Cells Dictate Spermatogonial Stem Cell Niches in the Mouse Testis

Sustained spermatogenesis in adult males relies on the activity of spermatogonial stem cells (SSCs). In general, tissue-specific stem cell populations such as SSCs are influenced by contributions of support cells that form niche microenvironments. Previous studies have provided indirect evidence tha...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Biology of reproduction Ročník 84; číslo 4; s. 639 - 645
Hlavní autori: Oatley, M. J., Racicot, K. E., Oatley, J. M.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Madison, WI Society for the Study of Reproduction 01.04.2011
Society for the Study of Reproduction, Inc
Predmet:
Adult Stem Cells - cytology
Adult Stem Cells - transplantation
Animals
Antithyroid Agents - pharmacology
Biological and medical sciences
Cell Count
Cell Differentiation
Cell Lineage
Cell Proliferation - drug effects
Fundamental and applied biological sciences. Psychology
Male
Mice
Mice, 129 Strain
Mice, Inbred C57BL
Mice, Transgenic
Propylthiouracil - pharmacology
Seminiferous Tubules - anatomy & histology
Seminiferous Tubules - blood supply
Seminiferous Tubules - drug effects
Sertoli Cells - cytology
Sertoli Cells - drug effects
Spermatogenesis
Spermatogonia - cytology
Spermatogonia - transplantation
Testis - blood supply
Testis - cytology
Testis - drug effects
Vertebrates: reproduction
Sertoli cell
spermatogonial stem cell
Spermatogenesis
Stem cell
Rodentia
Germinal cell
Testicle
Male genital system
Sertoli cells
Vertebrata
Reproduction
Mammalia
Mouse
Animal
Spermatogonia
niche
ISSN:0006-3363, 1529-7268, 1529-7268
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Sustained spermatogenesis in adult males relies on the activity of spermatogonial stem cells (SSCs). In general, tissue-specific stem cell populations such as SSCs are influenced by contributions of support cells that form niche microenvironments. Previous studies have provided indirect evidence that several somatic cell populations and the interstitial vasculature influence SSC functions, but an individual orchestrator of niches has not been described. In this study, functional transplantation of SSCs, in combination with experimental alteration of Sertoli cell content by polythiouracil (PTU)-induced transient hypothyroidism, was used to explore the relationship of Sertoli cells with SSCs in testes of adult mice. Transplantation of SSCs from PTU-treated donor mice into seminiferous tubules of normal recipient mice revealed a greater than 3-fold increase in SSCs compared to those from testes of non-PTU-treated donors. In addition, use of PTU-treated mice as recipients for transplantation of SSCs from normal donors revealed a greater than 3-fold increase of accessible niches compared to those of testes of non-PTU treated recipient mice with normal numbers of Sertoli cells. Importantly, the area of seminiferous tubules bordered by interstitial tissue and percentage of seminiferous tubules associated with blood vessels was found to be no different in testes of PTU-treated mice compared to controls, indicating that neither the vasculature nor interstitial support cell populations influenced the alteration of niche number. Collectively, these results provide direct evidence that Sertoli cells are the key somatic cell population dictating the number of SSCs and niches in mammalian testes.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-3363
1529-7268
1529-7268
DOI:10.1095/biolreprod.110.087320