Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: The case of a virus-resistant tomato

At present, the so‐called “substantial equivalence” is the only widely accepted criterion for deciding whether or not a transgenic food is, from an alimentary point of view, to be considered totally correspondent to the “traditional” one from which it derives. Although never exactly defined, it deal...

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Veröffentlicht in:	Proteomics (Weinheim) Jg. 4; H. 1; S. 193 - 200
Hauptverfasser:	Corpillo, Davide, Gardini, Giulia, Vaira, Anna Maria, Basso, Manuela, Aime, Silvio, Accotto, Gian Paolo, Fasano, Mauro
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Weinheim WILEY-VCH Verlag 01.01.2004 WILEY‐VCH Verlag Wiley-VCH
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Fundamental and applied biological sciences. Psychology Genetically modified organism Lycopersicon esculentum Lycopersicon esculentum - genetics Lycopersicon esculentum - physiology Lycopersicon esculentum - virology Microbiology Miscellaneous Plants, Genetically Modified - physiology Proteins Proteome - genetics Proteome - physiology Proteomics Substantial equivalence Terminology as Topic Tomato spotted wilt virus Tospovirus - physiology Virology Resistance Virus Dicotyledones Angiospermae Lycopersicon esculentum Proteomics Spermatophyta Solanaceae
ISSN:	1615-9853, 1615-9861
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Zusammenfassung:	At present, the so‐called “substantial equivalence” is the only widely accepted criterion for deciding whether or not a transgenic food is, from an alimentary point of view, to be considered totally correspondent to the “traditional” one from which it derives. Although never exactly defined, it deals with a comparison between the chemical composition of the two foods. A more in‐depth analysis can be performed by one of the most suitable methods that allows for the simultaneous screening of many components without prior identification, the analysis of the proteome. As a model for testing this kind of approach, we compared protein expression of two types of tomato plants, having the same genetic background, except for a virus resistance trait introduced by genetic engineering. When proteins extracted from seedlings of the two types were analyzed by two‐dimensional electrophoresis, no significant differences, either qualitative or quantitative, were detected, indicating that in this case the expression of major proteins was unmodified by the genetic manipulation. Fifteen proteins were identified by peptide mass fingerprinting.
Bibliographie:	ArticleID:PMIC200300540 ark:/67375/WNG-GQN3D0Z9-3 istex:52E43A4B3780A4CE867246A4CCF901CFBA056318 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1615-9853 1615-9861
DOI:	10.1002/pmic.200300540