The Mini‐Organo: A rapid high‐throughput 3D coculture organotypic assay for oncology screening and drug development
Background The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine‐tuned control of a range of microenvironmental elements in...
Saved in:
| Published in: | Cancer reports Vol. 3; no. 1; pp. e1209 - n/a |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
John Wiley and Sons Inc
01.02.2020
|
| Subjects: | |
| ISSN: | 2573-8348, 2573-8348 |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Background
The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine‐tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three‐dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease.
Aim
The development of the 3D coculture collagen contraction and invasion assay, the “organotypic assay,” has been widely adopted as a powerful approach to bridge the gap between standard two‐dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer‐associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low‐throughput, time‐consuming (up to several weeks), and work‐intensive with often limited scalability. Our aim was to develop a fast, high‐throughput, scalable 3D organotypic assay for use in oncology screening and drug development.
Methods and results
Here, we describe a modified 96‐well organotypic assay, the “Mini‐Organo,” which can be easily completed within 5 days. We demonstrate its application in a wide range of mouse and human cancer biology approaches including evaluation of stromal cell 3D ECM remodelling, 3D cancer cell invasion, and the assessment of efficacy of potential anticancer therapeutic targets. Furthermore, the organotypic assay described is highly amenable to customisation using different cell types under diverse experimental conditions.
Conclusions
The Mini‐Organo high‐throughput 3D organotypic assay allows the rapid screening of potential cancer therapeutics in human and mouse models in a time‐efficient manner. |
|---|---|
| AbstractList | The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine-tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three-dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease.BACKGROUNDThe use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine-tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three-dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease.The development of the 3D coculture collagen contraction and invasion assay, the "organotypic assay," has been widely adopted as a powerful approach to bridge the gap between standard two-dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer-associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low-throughput, time-consuming (up to several weeks), and work-intensive with often limited scalability. Our aim was to develop a fast, high-throughput, scalable 3D organotypic assay for use in oncology screening and drug development.AIMThe development of the 3D coculture collagen contraction and invasion assay, the "organotypic assay," has been widely adopted as a powerful approach to bridge the gap between standard two-dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer-associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low-throughput, time-consuming (up to several weeks), and work-intensive with often limited scalability. Our aim was to develop a fast, high-throughput, scalable 3D organotypic assay for use in oncology screening and drug development.Here, we describe a modified 96-well organotypic assay, the "Mini-Organo," which can be easily completed within 5 days. We demonstrate its application in a wide range of mouse and human cancer biology approaches including evaluation of stromal cell 3D ECM remodelling, 3D cancer cell invasion, and the assessment of efficacy of potential anticancer therapeutic targets. Furthermore, the organotypic assay described is highly amenable to customisation using different cell types under diverse experimental conditions.METHODS AND RESULTSHere, we describe a modified 96-well organotypic assay, the "Mini-Organo," which can be easily completed within 5 days. We demonstrate its application in a wide range of mouse and human cancer biology approaches including evaluation of stromal cell 3D ECM remodelling, 3D cancer cell invasion, and the assessment of efficacy of potential anticancer therapeutic targets. Furthermore, the organotypic assay described is highly amenable to customisation using different cell types under diverse experimental conditions.The Mini-Organo high-throughput 3D organotypic assay allows the rapid screening of potential cancer therapeutics in human and mouse models in a time-efficient manner.CONCLUSIONSThe Mini-Organo high-throughput 3D organotypic assay allows the rapid screening of potential cancer therapeutics in human and mouse models in a time-efficient manner. The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine-tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three-dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease. The development of the 3D coculture collagen contraction and invasion assay, the "organotypic assay," has been widely adopted as a powerful approach to bridge the gap between standard two-dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer-associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low-throughput, time-consuming (up to several weeks), and work-intensive with often limited scalability. Our aim was to develop a fast, high-throughput, scalable 3D organotypic assay for use in oncology screening and drug development. Here, we describe a modified 96-well organotypic assay, the "Mini-Organo," which can be easily completed within 5 days. We demonstrate its application in a wide range of mouse and human cancer biology approaches including evaluation of stromal cell 3D ECM remodelling, 3D cancer cell invasion, and the assessment of efficacy of potential anticancer therapeutic targets. Furthermore, the organotypic assay described is highly amenable to customisation using different cell types under diverse experimental conditions. The Mini-Organo high-throughput 3D organotypic assay allows the rapid screening of potential cancer therapeutics in human and mouse models in a time-efficient manner. Background The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine‐tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three‐dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease. Aim The development of the 3D coculture collagen contraction and invasion assay, the “organotypic assay,” has been widely adopted as a powerful approach to bridge the gap between standard two‐dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer‐associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low‐throughput, time‐consuming (up to several weeks), and work‐intensive with often limited scalability. Our aim was to develop a fast, high‐throughput, scalable 3D organotypic assay for use in oncology screening and drug development. Methods and results Here, we describe a modified 96‐well organotypic assay, the “Mini‐Organo,” which can be easily completed within 5 days. We demonstrate its application in a wide range of mouse and human cancer biology approaches including evaluation of stromal cell 3D ECM remodelling, 3D cancer cell invasion, and the assessment of efficacy of potential anticancer therapeutic targets. Furthermore, the organotypic assay described is highly amenable to customisation using different cell types under diverse experimental conditions. Conclusions The Mini‐Organo high‐throughput 3D organotypic assay allows the rapid screening of potential cancer therapeutics in human and mouse models in a time‐efficient manner. Background: The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine-tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three-dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease. Aim: The development of the 3D coculture collagen contraction and invasion assay, the "organotypic assay," has been widely adopted as a powerful approach to bridge the gap between standard two-dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer-associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low-throughput, time-consuming (up to several weeks), and work-intensive with often limited scalability. Our aim was to develop a fast, high-throughput, scalable 3D organotypic assay for use in oncology screening and drug development. Methods and results Here, we describe a modified 96-well organotypic assay, the "Mini-Organo," which can be easily completed within 5 days. We demonstrate its application in a wide range of mouse and human cancer biology approaches including evaluation of stromal cell 3D ECM remodelling, 3D cancer cell invasion, and the assessment of efficacy of potential anticancer therapeutic targets. Furthermore, the organotypic assay described is highly amenable to customisation using different cell types under diverse experimental conditions. Conclusions: The Mini-Organo high-throughput 3D organotypic assay allows the rapid screening of potential cancer therapeutics in human and mouse models in a time-efficient manner. |
| Author | Grant, Rhiannon D. Tayao, Michael Zaratzian, Anaiis Chitty, Jessica L. Cupello, Carmen Rodriguez Da Silva, Andrew M. Vennin, Claire Cox, Thomas R. Yam, Michelle Madsen, Chris D. Filipe, Elysse C. Major, Gretel Timpson, Paul Skhinas, Joanna N. Wang, Shan Papanicolaou, Michael |
| AuthorAffiliation | 2 St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney NSW Australia 4 School of Life Sciences University of Technology Sydney Sydney Australia 5 Molecular Pathology Oncode Institute, The Netherlands Cancer Institute Amsterdam The Netherlands 1 The Garvan Institute of Medical Research and the Kinghorn Cancer Centre Sydney NSW Australia 3 Department of Laboratory Medicine, Division of Translational Cancer Research Lund University Lund Sweden |
| AuthorAffiliation_xml | – name: 3 Department of Laboratory Medicine, Division of Translational Cancer Research Lund University Lund Sweden – name: 5 Molecular Pathology Oncode Institute, The Netherlands Cancer Institute Amsterdam The Netherlands – name: 1 The Garvan Institute of Medical Research and the Kinghorn Cancer Centre Sydney NSW Australia – name: 4 School of Life Sciences University of Technology Sydney Sydney Australia – name: 2 St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney NSW Australia |
| Author_xml | – sequence: 1 givenname: Jessica L. orcidid: 0000-0003-1776-1618 surname: Chitty fullname: Chitty, Jessica L. organization: St Vincent's Clinical School, Faculty of Medicine, UNSW – sequence: 2 givenname: Joanna N. orcidid: 0000-0001-8797-8228 surname: Skhinas fullname: Skhinas, Joanna N. – sequence: 3 givenname: Elysse C. orcidid: 0000-0003-1956-0852 surname: Filipe fullname: Filipe, Elysse C. organization: St Vincent's Clinical School, Faculty of Medicine, UNSW – sequence: 4 givenname: Shan surname: Wang fullname: Wang, Shan organization: Lund University – sequence: 5 givenname: Carmen Rodriguez orcidid: 0000-0003-3611-2496 surname: Cupello fullname: Cupello, Carmen Rodriguez organization: Lund University – sequence: 6 givenname: Rhiannon D. surname: Grant fullname: Grant, Rhiannon D. – sequence: 7 givenname: Michelle surname: Yam fullname: Yam, Michelle – sequence: 8 givenname: Michael surname: Papanicolaou fullname: Papanicolaou, Michael organization: University of Technology Sydney – sequence: 9 givenname: Gretel surname: Major fullname: Major, Gretel – sequence: 10 givenname: Anaiis surname: Zaratzian fullname: Zaratzian, Anaiis – sequence: 11 givenname: Andrew M. surname: Da Silva fullname: Da Silva, Andrew M. – sequence: 12 givenname: Michael surname: Tayao fullname: Tayao, Michael – sequence: 13 givenname: Claire orcidid: 0000-0001-9088-4177 surname: Vennin fullname: Vennin, Claire organization: Oncode Institute, The Netherlands Cancer Institute – sequence: 14 givenname: Paul orcidid: 0000-0002-5514-7080 surname: Timpson fullname: Timpson, Paul organization: St Vincent's Clinical School, Faculty of Medicine, UNSW – sequence: 15 givenname: Chris D. orcidid: 0000-0001-6838-2103 surname: Madsen fullname: Madsen, Chris D. email: chris.madsen@med.lu.se organization: Lund University – sequence: 16 givenname: Thomas R. orcidid: 0000-0001-9294-1745 surname: Cox fullname: Cox, Thomas R. email: t.cox@garvan.org.au organization: St Vincent's Clinical School, Faculty of Medicine, UNSW |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32671954$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1ks1u1DAUhSNUREvpghdAXtLFtLbj_LFAqoZfqVAJlfWVY98kRh472Emr2fEIPCNPguenqEWwsuX7nXN05fM0O3DeYZY9Z_SMUcrPlQv8jHHaPMqOeFHlizoX9cG9-2F2EuM3Simry5w3-ZPsMOdlxZpCHGW31wOST8aZXz9-XoVeOv-KXJAgR6PJYPohPU9D8HM_jPNE8jdEeTXbaQ5I_Baf1qNRRMYo16TzgXinvPX9mkQVEJ1xPZFOEx3mnmi8QevHFbrpWfa4kzbiyf48zr6-e3u9_LC4vHr_cXlxuVAFLZpF2zSctVh1lcZKKSqERsawbNuuKPOElJWkmjGqao552cq8FpWWsqC5VE3d5ceZ3PnGWxznFsZgVjKswUsDow-TtBAwogxqADtDREiUNUpOxrsIHdMlk6oF1rUaBCtaaBhrQVSoNNOyplynjNe7jCRdoVZpvZB8H0Q9mDgzQO9voGoEE0WTDF7uDYL_PmOcYGWiQmulQz9H4IILIaqyqBP64n7Wn5C7H03A-Q5QwccYsANlpu02KdpYYBQ2tYFNbWBTm6Q4_UtxZ_ovdu9-ayyu_w_C8vMXvlX8BtKB2Co |
| CitedBy_id | crossref_primary_10_1002_advs_202307963 crossref_primary_10_3389_fdgth_2021_704584 crossref_primary_10_1038_s41563_021_01087_z crossref_primary_10_1002_btm2_10741 crossref_primary_10_1038_s41467_022_32255_7 crossref_primary_10_3390_ijms22031195 crossref_primary_10_1038_s43018_023_00614_y |
| Cites_doi | 10.1038/nm.4352 10.4155/fmc-2016-0025 10.1016/j.tiv.2018.05.006 10.1101/sqb.2016.81.030817 10.1046/j.1432-0436.2002.700907.x 10.15252/embr.201540107 10.1186/s13058-015-0592-1 10.1016/j.ceb.2010.08.015 10.1038/nprot.2006.430 10.1038/ki.1991.310 10.1038/ncb2756 10.1038/s41568-018-0006-7 10.1158/1078-0432.CCR-13-1059 10.1083/jcb.201210152 10.1529/biophysj.106.097998 10.1038/nrc1877 10.1038/s41467-018-05220-6 10.1126/scitranslmed.aai8504 10.3389/fphar.2014.00118 10.1039/c5ib00040h 10.1038/onc.2017.49 10.1038/ncb1658 10.1016/j.trecan.2016.04.005 10.1038/nmeth.2019 10.1083/jcb.201704053 10.1177/1087057104265040 10.1158/1541-7786.MCR-15-0307 10.1242/dmm.029447 10.1093/carcin/bgu108 10.1016/j.pan.2015.02.004 10.1038/ncb3478 10.1007/978-1-4939-7021-6_15 10.1016/j.trecan.2016.05.004 10.1016/j.ccr.2014.05.026 10.1016/j.ceb.2016.05.002 10.1172/JCI115821 10.1053/j.gastro.2017.11.280 10.1038/nrc3319 10.2478/tumor-2012-0002 10.1016/0165-022X(95)00046-T 10.1172/JCI116631 10.1016/j.cell.2017.11.010 10.1136/gutjnl-2017-315144 10.1111/bph.14195 10.1038/nrm3459 10.1007/BF01002772 10.1038/s41598-017-17204-5 10.1016/j.ccr.2009.12.041 10.1038/nrc.2016.73 10.1016/j.ccr.2005.04.023 10.1369/0022155414545787 10.1016/j.biomaterials.2010.03.039 10.1111/iep.12269 10.1039/C0AN00609B 10.1002/jbio.200910062 10.1016/j.tcb.2016.08.008 10.1038/s41598-018-31138-6 10.1038/sj.bjc.6605469 10.1056/NEJM200005043421807 10.12688/f1000research.15064.2 10.1016/j.tibtech.2015.01.004 10.1038/nrm3873 10.1016/j.semcdb.2009.10.002 10.4155/fmc.11.169 10.1073/pnas.0908428107 10.1111/j.1365-2133.1995.tb02619.x 10.1091/mbc.e17-05-0320 10.1126/scitranslmed.aau7377 10.1016/j.tcb.2016.08.007 10.1242/dmm.004077 10.1038/s41598-017-17177-5 |
| ContentType | Journal Article |
| Copyright | 2019 The Authors Cancer Reports Published by Wiley Periodicals, Inc. |
| Copyright_xml | – notice: 2019 The Authors Cancer Reports Published by Wiley Periodicals, Inc. |
| CorporateAuthor | LUCC: Lunds universitets cancercentrum Avdelningen för translationell cancerforskning Övriga starka forskningsmiljöer Division of Translational Cancer Research Profile areas and other strong research environments Lunds universitet Department of Laboratory Medicine Lund University Institutionen för laboratoriemedicin Other Strong Research Environments Faculty of Medicine LUCC: Lund University Cancer Centre Medicinska fakulteten Profilområden och andra starka forskningsmiljöer |
| CorporateAuthor_xml | – name: Övriga starka forskningsmiljöer – name: Faculty of Medicine – name: Medicinska fakulteten – name: Other Strong Research Environments – name: LUCC: Lund University Cancer Centre – name: Department of Laboratory Medicine – name: Division of Translational Cancer Research – name: Lunds universitet – name: Profilområden och andra starka forskningsmiljöer – name: Lund University – name: Avdelningen för translationell cancerforskning – name: Institutionen för laboratoriemedicin – name: Profile areas and other strong research environments – name: LUCC: Lunds universitets cancercentrum |
| DBID | 24P AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 5PM ADTPV AGCHP AOWAS D8T D95 ZZAVC |
| DOI | 10.1002/cnr2.1209 |
| DatabaseName | Wiley Online Library Open Access (Activated by CARLI) CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic PubMed Central (Full Participant titles) SwePub SWEPUB Lunds universitet full text SwePub Articles SWEPUB Freely available online SWEPUB Lunds universitet SwePub Articles full text |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: 24P name: Wiley Online Library Open Access url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html sourceTypes: Publisher – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Chitty et al |
| EISSN | 2573-8348 |
| EndPage | n/a |
| ExternalDocumentID | oai_portal_research_lu_se_publications_f1d61acb_1fbd_415b_911b_47ecd1da802d PMC7941459 32671954 10_1002_cnr2_1209 CNR21209 |
| Genre | caseStudy Research Support, Non-U.S. Gov't Journal Article |
| GrantInformation_xml | – fundername: Åke Wiberg foundation funderid: M17‐0235 – fundername: BioCARE – fundername: National Health and Medical Research Council (NHMRC) funderid: 1129766; 1140125; 1158590 – fundername: Susan G Komen funderid: 1748329 – fundername: Swedish Research Council funderid: 2017‐03389 – fundername: Cancerfonden funderid: CAN 2016/783 – fundername: Human Frontier Science Program funderid: CV – fundername: Avner Pancreatic Cancer Foundation funderid: R3‐PT – fundername: Crafoord Foundation funderid: 20171049 – fundername: Cancer Institute NSW funderid: 171105 – fundername: ; grantid: N91/15 – fundername: ; grantid: 171105 – fundername: ; grantid: 20171049 – fundername: ; grantid: RG 19‐09 – fundername: ; grantid: CV – fundername: ; grantid: 2017‐03389 – fundername: ; grantid: 1129766; 1140125; 1158590 – fundername: ; grantid: 1748329 – fundername: ; grantid: M17‐0235 |
| GroupedDBID | 0R~ 1OC 24P 53G 7X7 88E 8FI 8FJ AAMMB AAZKR ABCUV ABUWG ACCMX ACGFS ACPOU ACXQS ADBBV ADKYN ADPDF ADXAS ADZMN AEFGJ AFKRA AGXDD AIDQK AIDYY AIURR ALMA_UNASSIGNED_HOLDINGS AMYDB AVUZU BENPR BFHJK CCPQU DCZOG EBS EJD FYUFA GROUPED_DOAJ HMCUK IAO IHR INH ITC LATKE LEEKS LUTES LYRES M1P M~E O9- OK1 OVD OVEED P2W PHGZM PHGZT PIMPY PSQYO ROL RPM SUPJJ TEORI UKHRP ZZTAW AAYXX AFFHD ALUQN CITATION PJZUB PPXIY WIN CGR CUY CVF ECM EIF NPM 7X8 5PM ADTPV AGCHP AOWAS D8T D95 ZZAVC |
| ID | FETCH-LOGICAL-c5059-b9921be7f7de7cc044de11e6bbf56350567a0d110c82e36ba3847daa503ac98f3 |
| IEDL.DBID | 24P |
| ISICitedReferencesCount | 9 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000588169600005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2573-8348 |
| IngestDate | Sat Nov 01 14:37:39 EDT 2025 Tue Nov 04 01:47:22 EST 2025 Thu Sep 04 18:34:38 EDT 2025 Mon Jul 21 06:01:44 EDT 2025 Sat Nov 29 06:40:31 EST 2025 Tue Nov 18 21:02:50 EST 2025 Sun Jul 06 04:45:03 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | cancer-associated fibroblast 3D model coculture organotypic drug screening extracellular matrix |
| Language | English |
| License | Attribution-NonCommercial 2019 The Authors Cancer Reports Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c5059-b9921be7f7de7cc044de11e6bbf56350567a0d110c82e36ba3847daa503ac98f3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 The copyright line for this article was changed on 2 August 2019 after original online publication. |
| ORCID | 0000-0001-6838-2103 0000-0003-1956-0852 0000-0002-5514-7080 0000-0003-1776-1618 0000-0003-3611-2496 0000-0001-8797-8228 0000-0001-9088-4177 0000-0001-9294-1745 |
| OpenAccessLink | https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fcnr2.1209 |
| PMID | 32671954 |
| PQID | 2424447658 |
| PQPubID | 23479 |
| PageCount | 15 |
| ParticipantIDs | swepub_primary_oai_portal_research_lu_se_publications_f1d61acb_1fbd_415b_911b_47ecd1da802d pubmedcentral_primary_oai_pubmedcentral_nih_gov_7941459 proquest_miscellaneous_2424447658 pubmed_primary_32671954 crossref_citationtrail_10_1002_cnr2_1209 crossref_primary_10_1002_cnr2_1209 wiley_primary_10_1002_cnr2_1209_CNR21209 |
| PublicationCentury | 2000 |
| PublicationDate | February 2020 |
| PublicationDateYYYYMMDD | 2020-02-01 |
| PublicationDate_xml | – month: 02 year: 2020 text: February 2020 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Hoboken |
| PublicationTitle | Cancer reports |
| PublicationTitleAlternate | Cancer Rep (Hoboken) |
| PublicationYear | 2020 |
| Publisher | John Wiley and Sons Inc |
| Publisher_xml | – name: John Wiley and Sons Inc |
| References | 2017; 7 2005; 294 2010; 107 2010; 17 2015; 33 2004; 9 2010; 102 2014; 25 2013; 201 2011; 13 1995; 133 2012; 13 2014; 62 2012; 12 1996; 32 2017; 9 2014; 20 2018; 7 2010; 22 1992; 90 2010; 21 2018; 9 2018; 176 2018; 8 2014; 5 2013; 15 2018; 172 2017; 36 1991; 40 2016; 42 2014; 15 2007; 9 2016; 81 2010; 3 2011; 136 2015; 15 2010; 31 2015; 17 2015; 16 2017; 28 2017; 23 2007; 92 2006; 6 2006; 1 2011; 4 2018; 67 2016; 16 1979; 11 2015; 7 2016; 14 2017; 216 2018; 18 2018; 154 2016; 2 2012; 1 2017; 1612 1993; 92 2005; 7 2014; 35 2002; 70 2017; 19 2018; 51 2000; 342 2018; 11 2018; 99 2018; 10 2012; 4 2016; 26 2016; 8 2012; 9 e_1_2_10_23_1 e_1_2_10_46_1 e_1_2_10_69_1 e_1_2_10_21_1 e_1_2_10_44_1 e_1_2_10_42_1 e_1_2_10_40_1 e_1_2_10_70_1 e_1_2_10_2_1 e_1_2_10_72_1 e_1_2_10_4_1 e_1_2_10_18_1 e_1_2_10_74_1 e_1_2_10_53_1 e_1_2_10_6_1 e_1_2_10_16_1 e_1_2_10_39_1 e_1_2_10_55_1 e_1_2_10_8_1 e_1_2_10_14_1 e_1_2_10_37_1 e_1_2_10_57_1 e_1_2_10_58_1 e_1_2_10_13_1 e_1_2_10_34_1 e_1_2_10_11_1 e_1_2_10_32_1 Cukierman E (e_1_2_10_66_1) 2005; 294 e_1_2_10_30_1 e_1_2_10_51_1 e_1_2_10_61_1 e_1_2_10_29_1 Timpson P (e_1_2_10_43_1) 2011; 13 e_1_2_10_63_1 e_1_2_10_27_1 e_1_2_10_65_1 e_1_2_10_25_1 e_1_2_10_48_1 e_1_2_10_67_1 e_1_2_10_24_1 e_1_2_10_45_1 e_1_2_10_22_1 e_1_2_10_20_1 e_1_2_10_41_1 e_1_2_10_71_1 e_1_2_10_73_1 e_1_2_10_52_1 e_1_2_10_3_1 e_1_2_10_19_1 e_1_2_10_54_1 e_1_2_10_5_1 e_1_2_10_17_1 e_1_2_10_38_1 e_1_2_10_56_1 e_1_2_10_7_1 e_1_2_10_15_1 e_1_2_10_36_1 e_1_2_10_12_1 e_1_2_10_35_1 e_1_2_10_9_1 e_1_2_10_59_1 e_1_2_10_10_1 e_1_2_10_33_1 e_1_2_10_31_1 e_1_2_10_50_1 e_1_2_10_60_1 e_1_2_10_62_1 e_1_2_10_64_1 e_1_2_10_28_1 e_1_2_10_49_1 e_1_2_10_26_1 e_1_2_10_47_1 e_1_2_10_68_1 |
| References_xml | – volume: 16 start-page: 582 issue: 9 year: 2016 end-page: 598 article-title: The biology and function of fibroblasts in cancer publication-title: Nat Rev Cancer – volume: 9 start-page: 1392 issue: 12 year: 2007 end-page: 1400 article-title: Fibroblast‐led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells publication-title: Nat Cell Biol – volume: 9 start-page: 2897 issue: 1 year: 2018 article-title: Targeting stromal remodeling and cancer stem cell plasticity overcomes chemoresistance in triple negative breast cancer publication-title: Nat Commun – volume: 2 start-page: 279 issue: 6 year: 2016 end-page: 282 article-title: Fibrosis and cancer: partners in crime or opposing forces? publication-title: Trends Cancer – volume: 11 start-page: 447 issue: 4 year: 1979 end-page: 455 article-title: Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections publication-title: Histochem J – volume: 1 start-page: 2753 issue: 6 year: 2006 end-page: 2758 article-title: Preparation of ready‐to‐use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications publication-title: Nat Protoc – volume: 92 start-page: 2212 issue: 6 year: 2007 end-page: 2222 article-title: Noninvasive assessment of collagen gel microstructure and mechanics using multiphoton microscopy publication-title: Biophys J – volume: 7 start-page: 469 issue: 5 year: 2005 end-page: 483 article-title: Trp53R172H and KrasG12D cooperate to promote chromosomal instability and widely metastatic pancreatic ductal adenocarcinoma in mice publication-title: Cancer Cell – volume: 294 start-page: 79 year: 2005 end-page: 93 article-title: Cell migration analyses within fibroblast‐derived 3‐D matrices publication-title: Methods Mol Biol – volume: 21 start-page: 19 issue: 1 year: 2010 end-page: 25 article-title: Carcinoma‐associated fibroblasts are a rate‐limiting determinant for tumour progression publication-title: Semin Cell Dev Biol – volume: 67 start-page: 2142 issue: 12 year: 2018 end-page: 2155 article-title: Tailored first‐line and second‐line CDK4‐targeting treatment combinations in mouse models of pancreatic cancer publication-title: Gut – volume: 201 start-page: 1069 issue: 7 year: 2013 end-page: 1084 article-title: Physical limits of cell migration: control by ECM space and nuclear deformation and tuning by proteolysis and traction force publication-title: J Cell Biol – volume: 23 start-page: 890 issue: 7 year: 2017 end-page: 898 article-title: ISDoT: in situ decellularization of tissues for high‐resolution imaging and proteomic analysis of native extracellular matrix publication-title: Nat Med – volume: 51 start-page: 83 year: 2018 end-page: 94 article-title: High‐throughput toxicity testing of chemicals and mixtures in organotypic multi‐cellular cultures of primary human hepatic cells publication-title: Toxicol In Vitro – volume: 15 start-page: 647 issue: 10 year: 2014 end-page: 664 article-title: Three‐dimensional organotypic culture: experimental models of mammalian biology and disease publication-title: Nat Rev Mol Cell Biol – volume: 9 start-page: 273 issue: 4 year: 2004 end-page: 285 article-title: The use of 3‐D cultures for high‐throughput screening: the multicellular spheroid model publication-title: J Biomol Screen – volume: 133 start-page: 223 issue: 2 year: 1995 end-page: 230 article-title: Effects of retinoids on glycosaminoglycan synthesis by human skin fibroblasts grown as monolayers and within contracted collagen lattices publication-title: Br J Dermatol – volume: 90 start-page: 1 issue: 1 year: 1992 end-page: 7 article-title: Transforming growth factor‐beta in disease: the dark side of tissue repair publication-title: J Clin Invest – volume: 26 start-page: 794 year: 2016 end-page: 795 article-title: Ingber: engineering the culture microenvironment publication-title: Trends Cell Biol – volume: 17 start-page: 79 issue: 1 year: 2015 article-title: High mammographic density is associated with an increase in stromal collagen and immune cells within the mammary epithelium publication-title: Breast Cancer Res – volume: 42 start-page: 80 year: 2016 end-page: 93 article-title: Stromal dynamic reciprocity in cancer: intricacies of fibroblastic‐ECM interactions publication-title: Curr Opin Cell Biol – volume: 18 start-page: 359 issue: 6 year: 2018 end-page: 376 article-title: A history of exploring cancer in context publication-title: Nat Rev Cancer – volume: 107 start-page: 246 issue: 1 year: 2010 end-page: 251 article-title: Mutant p53 drives metastasis and overcomes growth arrest/senescence in pancreatic cancer publication-title: Proc Natl Acad Sci U S A – volume: 70 start-page: 537 issue: 9‐10 year: 2002 end-page: 546 article-title: The organizing principle: microenvironmental influences in the normal and malignant breast publication-title: Differentiation – volume: 102 start-page: 392 issue: 2 year: 2010 end-page: 402 article-title: A chemical biology screen reveals a role for Rab21‐mediated control of actomyosin contractility in fibroblast‐driven cancer invasion publication-title: Br J Cancer – volume: 342 start-page: 1350 issue: 18 year: 2000 end-page: 1358 article-title: Role of transforming growth factor beta in human disease publication-title: N Engl J Med – volume: 40 start-page: 1020 issue: 6 year: 1991 end-page: 1031 article-title: Pathogenesis of interstitial fibrosis in chronic purine aminonucleoside nephrosis publication-title: Kidney Int – volume: 2 start-page: 277 issue: 6 year: 2016 end-page: 279 article-title: Cancer‐associated fibroblasts: perspectives in cancer therapy publication-title: Trends Cancer – volume: 14 start-page: 287 issue: 3 year: 2016 end-page: 295 article-title: Cancer‐associated fibroblasts induce a collagen cross‐link switch in tumor stroma publication-title: Mol Cancer Res – volume: 7 start-page: 1120 issue: 10 year: 2015 end-page: 1134 article-title: Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration publication-title: Integr Biol (Camb) – volume: 7 start-page: 16887 issue: 1 year: 2017 article-title: Three‐dimensional organotypic matrices from alternative collagen sources as pre‐clinical models for cell biology publication-title: Sci Rep – volume: 4 start-page: 87 issue: 1 year: 2012 end-page: 105 article-title: Advancing cancer drug discovery towards more agile development of targeted combination therapies publication-title: Future Med Chem – volume: 7 start-page: pii: F1000 Faculty Rev‐1169 year: 2018 article-title: Recent advances in understanding the complexities of metastasis. [version 2; peer review: 3 approved] publication-title: F1000Res – volume: 172 start-page: 373 issue: 1‐2 year: 2018 end-page: 386 article-title: A living biobank of breast cancer organoids captures disease heterogeneity publication-title: Cell – volume: 136 start-page: 473 issue: 3 year: 2011 end-page: 478 article-title: High‐throughput 3D spheroid culture and drug testing using a 384 hanging drop array publication-title: Analyst – volume: 176 start-page: 82 year: 2018 end-page: 92 article-title: The extracellular matrix as a key regulator of intracellular signalling networks publication-title: Br J Pharmacol – volume: 33 start-page: 230 issue: 4 year: 2015 end-page: 236 article-title: Biomechanical and biochemical remodeling of stromal extracellular matrix in cancer publication-title: Trends Biotechnol – volume: 216 start-page: 3799 issue: 11 year: 2017 end-page: 3816 article-title: Cancer‐associated fibroblasts promote directional cancer cell migration by aligning fibronectin publication-title: J Cell Biol – volume: 28 start-page: 1815 issue: 14 year: 2017 end-page: 1818 article-title: Cell and tissue mechanics: the new cell biology frontier publication-title: Mol Biol Cell – volume: 16 start-page: 1394 issue: 10 year: 2015 end-page: 1408 article-title: Hypoxia and loss of PHD2 inactivate stromal fibroblasts to decrease tumour stiffness and metastasis publication-title: EMBO Rep – volume: 15 start-page: 200 issue: 2 year: 2015 end-page: 201 article-title: Imaging fibrosis in pancreatic cancer using second harmonic generation publication-title: Pancreatology – volume: 1612 start-page: 199 year: 2017 end-page: 212 article-title: Analysis of breast cancer cell invasion using an organotypic culture system publication-title: Methods Mol Biol – volume: 22 start-page: 697 issue: 5 year: 2010 end-page: 706 article-title: Dynamic interplay between the collagen scaffold and tumor evolution publication-title: Curr Opin Cell Biol – volume: 26 start-page: 798 issue: 11 year: 2016 end-page: 800 article-title: 3D biomimetic cultures: the next platform for cell biology publication-title: Trends Cell Biol – volume: 15 start-page: 637 issue: 6 year: 2013 end-page: 646 article-title: Mechanotransduction and YAP‐dependent matrix remodelling is required for the generation and maintenance of cancer‐associated fibroblasts publication-title: Nat Cell Biol – volume: 3 start-page: 34 issue: 1‐2 year: 2010 end-page: 43 article-title: Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy publication-title: J Biophotonics – volume: 35 start-page: 1671 issue: 8 year: 2014 end-page: 1679 article-title: Three‐dimensional cancer models mimic cell‐matrix interactions in the tumour microenvironment publication-title: Carcinogenesis – volume: 32 start-page: 33 issue: 1 year: 1996 end-page: 43 article-title: Modification of Lowry's method for collagen concentration measurement publication-title: J Biochem Biophys Methods – volume: 8 start-page: 13039 year: 2018 article-title: Development of a novel 3D tumor‐tissue invasion model for high‐throughput, High‐Content Phenotypic Drug Screening publication-title: Sci Rep – volume: 13 issue: 56 year: 2011 article-title: Organotypic collagen I assay: a malleable platform to assess cell behaviour in a 3‐dimensional context publication-title: J Vis Exp – volume: 7 start-page: 16878 issue: 1 year: 2017 article-title: QuPath: open source software for digital pathology image analysis publication-title: Sci Rep – volume: 11 start-page: dmm029447 issue: 4 year: 2018 article-title: A peek into cancer‐associated fibroblasts: origins, functions and translational impact publication-title: Dis Model Mech – volume: 1 start-page: 14 year: 2012 end-page: 18 article-title: Network biology and the 3‐dimensional tumor microenvironment: personalizing medicine for the future: tumor microenvironment and therapy publication-title: Tumor Microenviron Ther – volume: 5 start-page: 118 year: 2014 article-title: Quantitative phenotypic and pathway profiling guides rational drug combination strategies publication-title: Front Pharmacol – volume: 10 start-page: eaau7377 issue: 451 year: 2018 article-title: Patient‐derived organoids: are PDOs the new PDX? publication-title: Sci Transl Med – volume: 8 start-page: 1331 issue: 11 year: 2016 end-page: 1347 article-title: Next‐generation phenotypic screening publication-title: Future Med Chem – volume: 31 start-page: 5678 issue: 21 year: 2010 end-page: 5688 article-title: Pore size variable type I collagen gels and their interaction with glioma cells publication-title: Biomaterials – volume: 12 start-page: 540 issue: 8 year: 2012 end-page: 552 article-title: The rationale for targeting the LOX family in cancer publication-title: Nat Rev Cancer – volume: 81 start-page: 189 year: 2016 end-page: 205 article-title: Physical and chemical gradients in the tumor microenvironment regulate tumor cell invasion, migration, and metastasis publication-title: Cold Spring Harb Symp Quant Biol – volume: 20 start-page: 3637 issue: 14 year: 2014 end-page: 3643 article-title: Molecular pathways: connecting fibrosis and solid tumor metastasis publication-title: Clin Cancer Res – volume: 9 start-page: eaai8504 issue: 384 year: 2017 article-title: Transient tissue priming via ROCK inhibition uncouples pancreatic cancer progression, sensitivity to chemotherapy, and metastasis publication-title: Sci Transl Med – volume: 13 start-page: 743 issue: 11 year: 2012 end-page: 747 article-title: New dimensions in cell migration publication-title: Nat Rev Mol Cell Biol – volume: 99 start-page: 58 issue: 2 year: 2018 end-page: 76 article-title: Charting the unexplored extracellular matrix in cancer publication-title: Int J Exp Pathol – volume: 92 start-page: 632 issue: 2 year: 1993 end-page: 637 article-title: Altered expression of small proteoglycans, collagen, and transforming growth factor‐beta 1 in developing bleomycin‐induced pulmonary fibrosis in rats publication-title: J Clin Invest – volume: 25 start-page: 711 issue: 6 year: 2014 end-page: 712 article-title: Pancreatic cancer stroma: friend or foe? publication-title: Cancer Cell – volume: 19 start-page: 224 issue: 3 year: 2017 end-page: 237 article-title: A mechanically active heterotypic E‐cadherin/N‐cadherin adhesion enables fibroblasts to drive cancer cell invasion publication-title: Nat Cell Biol – volume: 4 start-page: 165 issue: 2 year: 2011 end-page: 178 article-title: Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer publication-title: Dis Model Mech – volume: 62 start-page: 751 issue: 10 year: 2014 end-page: 758 article-title: Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues publication-title: J Histochem Cytochem – volume: 6 start-page: 392 issue: 5 year: 2006 end-page: 401 article-title: Fibroblasts in cancer publication-title: Nat Rev Cancer – volume: 154 start-page: 820 issue: 4 year: 2018 end-page: 838 article-title: Reshaping the tumor stroma for treatment of pancreatic cancer publication-title: Gastroenterology – volume: 36 start-page: 4434 issue: 31 year: 2017 end-page: 4444 article-title: Cancer‐associated fibroblasts induce cancer cell apoptosis that regulates invasion mode of tumours publication-title: Oncogene – volume: 9 start-page: 676 issue: 7 year: 2012 end-page: 682 article-title: Fiji: an open‐source platform for biological‐image analysis publication-title: Nat Methods – volume: 17 start-page: 135 issue: 2 year: 2010 end-page: 147 article-title: Cancer‐associated fibroblasts are activated in incipient neoplasia to orchestrate tumor‐promoting inflammation in an NF‐kappaB‐dependent manner publication-title: Cancer Cell – ident: e_1_2_10_61_1 doi: 10.1038/nm.4352 – ident: e_1_2_10_22_1 doi: 10.4155/fmc-2016-0025 – ident: e_1_2_10_17_1 doi: 10.1016/j.tiv.2018.05.006 – ident: e_1_2_10_29_1 doi: 10.1101/sqb.2016.81.030817 – ident: e_1_2_10_31_1 doi: 10.1046/j.1432-0436.2002.700907.x – ident: e_1_2_10_52_1 doi: 10.15252/embr.201540107 – ident: e_1_2_10_64_1 doi: 10.1186/s13058-015-0592-1 – ident: e_1_2_10_32_1 doi: 10.1016/j.ceb.2010.08.015 – ident: e_1_2_10_46_1 doi: 10.1038/nprot.2006.430 – ident: e_1_2_10_73_1 doi: 10.1038/ki.1991.310 – ident: e_1_2_10_51_1 doi: 10.1038/ncb2756 – ident: e_1_2_10_27_1 doi: 10.1038/s41568-018-0006-7 – ident: e_1_2_10_41_1 doi: 10.1158/1078-0432.CCR-13-1059 – ident: e_1_2_10_54_1 doi: 10.1083/jcb.201210152 – ident: e_1_2_10_56_1 doi: 10.1529/biophysj.106.097998 – ident: e_1_2_10_70_1 doi: 10.1038/nrc1877 – ident: e_1_2_10_62_1 doi: 10.1038/s41467-018-05220-6 – ident: e_1_2_10_59_1 doi: 10.1126/scitranslmed.aai8504 – ident: e_1_2_10_21_1 doi: 10.3389/fphar.2014.00118 – ident: e_1_2_10_30_1 doi: 10.1039/c5ib00040h – ident: e_1_2_10_33_1 doi: 10.1038/onc.2017.49 – ident: e_1_2_10_49_1 doi: 10.1038/ncb1658 – volume: 13 start-page: e3089 issue: 56 year: 2011 ident: e_1_2_10_43_1 article-title: Organotypic collagen I assay: a malleable platform to assess cell behaviour in a 3‐dimensional context publication-title: J Vis Exp – ident: e_1_2_10_39_1 doi: 10.1016/j.trecan.2016.04.005 – ident: e_1_2_10_68_1 doi: 10.1038/nmeth.2019 – ident: e_1_2_10_35_1 doi: 10.1083/jcb.201704053 – ident: e_1_2_10_18_1 doi: 10.1177/1087057104265040 – ident: e_1_2_10_34_1 doi: 10.1158/1541-7786.MCR-15-0307 – ident: e_1_2_10_3_1 doi: 10.1242/dmm.029447 – ident: e_1_2_10_7_1 doi: 10.1093/carcin/bgu108 – ident: e_1_2_10_63_1 doi: 10.1016/j.pan.2015.02.004 – ident: e_1_2_10_36_1 doi: 10.1038/ncb3478 – ident: e_1_2_10_45_1 doi: 10.1007/978-1-4939-7021-6_15 – volume: 294 start-page: 79 year: 2005 ident: e_1_2_10_66_1 article-title: Cell migration analyses within fibroblast‐derived 3‐D matrices publication-title: Methods Mol Biol – ident: e_1_2_10_26_1 doi: 10.1016/j.trecan.2016.05.004 – ident: e_1_2_10_24_1 doi: 10.1016/j.ccr.2014.05.026 – ident: e_1_2_10_2_1 doi: 10.1016/j.ceb.2016.05.002 – ident: e_1_2_10_72_1 doi: 10.1172/JCI115821 – ident: e_1_2_10_23_1 doi: 10.1053/j.gastro.2017.11.280 – ident: e_1_2_10_69_1 doi: 10.1038/nrc3319 – ident: e_1_2_10_8_1 doi: 10.2478/tumor-2012-0002 – ident: e_1_2_10_53_1 doi: 10.1016/0165-022X(95)00046-T – ident: e_1_2_10_74_1 doi: 10.1172/JCI116631 – ident: e_1_2_10_12_1 doi: 10.1016/j.cell.2017.11.010 – ident: e_1_2_10_60_1 doi: 10.1136/gutjnl-2017-315144 – ident: e_1_2_10_9_1 doi: 10.1111/bph.14195 – ident: e_1_2_10_10_1 doi: 10.1038/nrm3459 – ident: e_1_2_10_58_1 doi: 10.1007/BF01002772 – ident: e_1_2_10_67_1 doi: 10.1038/s41598-017-17204-5 – ident: e_1_2_10_37_1 doi: 10.1016/j.ccr.2009.12.041 – ident: e_1_2_10_38_1 doi: 10.1038/nrc.2016.73 – ident: e_1_2_10_47_1 doi: 10.1016/j.ccr.2005.04.023 – ident: e_1_2_10_57_1 doi: 10.1369/0022155414545787 – ident: e_1_2_10_55_1 doi: 10.1016/j.biomaterials.2010.03.039 – ident: e_1_2_10_4_1 doi: 10.1111/iep.12269 – ident: e_1_2_10_19_1 doi: 10.1039/C0AN00609B – ident: e_1_2_10_65_1 doi: 10.1002/jbio.200910062 – ident: e_1_2_10_15_1 doi: 10.1016/j.tcb.2016.08.008 – ident: e_1_2_10_16_1 doi: 10.1038/s41598-018-31138-6 – ident: e_1_2_10_50_1 doi: 10.1038/sj.bjc.6605469 – ident: e_1_2_10_71_1 doi: 10.1056/NEJM200005043421807 – ident: e_1_2_10_5_1 doi: 10.12688/f1000research.15064.2 – ident: e_1_2_10_25_1 doi: 10.1016/j.tibtech.2015.01.004 – ident: e_1_2_10_6_1 doi: 10.1038/nrm3873 – ident: e_1_2_10_40_1 doi: 10.1016/j.semcdb.2009.10.002 – ident: e_1_2_10_20_1 doi: 10.4155/fmc.11.169 – ident: e_1_2_10_48_1 doi: 10.1073/pnas.0908428107 – ident: e_1_2_10_44_1 doi: 10.1111/j.1365-2133.1995.tb02619.x – ident: e_1_2_10_28_1 doi: 10.1091/mbc.e17-05-0320 – ident: e_1_2_10_13_1 doi: 10.1126/scitranslmed.aau7377 – ident: e_1_2_10_14_1 doi: 10.1016/j.tcb.2016.08.007 – ident: e_1_2_10_42_1 doi: 10.1242/dmm.004077 – ident: e_1_2_10_11_1 doi: 10.1038/s41598-017-17177-5 |
| SSID | ssj0001863293 |
| Score | 2.1704266 |
| Snippet | Background
The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal... The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease... Background: The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal... |
| SourceID | swepub pubmedcentral proquest pubmed crossref wiley |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | e1209 |
| SubjectTerms | 3D model Animals Basic Medicine Cancer-Associated Fibroblasts - physiology cancer‐associated fibroblast Cell and Molecular Biology Cell- och molekylärbiologi coculture Coculture Techniques - methods Drug Development - methods drug screening Drug Screening Assays, Antitumor - methods extracellular matrix High-Throughput Screening Assays Humans Medical and Health Sciences Medicin och hälsovetenskap Medicinska och farmaceutiska grundvetenskaper Method Report Mice Neoplasm Invasiveness organotypic Rats |
| Title | The Mini‐Organo: A rapid high‐throughput 3D coculture organotypic assay for oncology screening and drug development |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fcnr2.1209 https://www.ncbi.nlm.nih.gov/pubmed/32671954 https://www.proquest.com/docview/2424447658 https://pubmed.ncbi.nlm.nih.gov/PMC7941459 |
| Volume | 3 |
| WOSCitedRecordID | wos000588169600005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2573-8348 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001863293 issn: 2573-8348 databaseCode: M~E dateStart: 20180101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVWIB databaseName: Wiley - Free Content customDbUrl: eissn: 2573-8348 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001863293 issn: 2573-8348 databaseCode: WIN dateStart: 20180101 isFulltext: true titleUrlDefault: https://onlinelibrary.wiley.com providerName: Wiley-Blackwell |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3LbtQwFLVKYcGGh3gNLxnEgk1obCexA6uqtIJFRxUCMWJj-RUaqUqizKTVbBCfwDfyJVw7mQxRQUJiY0WJkyj2ub7nOva5CL3gNKU25y6KqYUABQKGKOcpjfwqNgcMPU-MDckm-HwuFov8ZAe92eyF6fUhxgk3bxlhvPYGrvRybysaaqqWvvI7P6-gq4Qw7iFNk5PtBIvIGA2iu4BKFgmWiI2yUEz3xrun_ugSyby8VnJQFJ2S2eCNjm7-13fcQjcGEor3e9TcRjuuuoMuADH4uKzKn99_hB2a9Wu8j1vVlBZ7UWM4PST1aboVZm8xDKVBtsPhkBqqXq2b0mAg42qNgQrjugqK2GsMIxNEy-Ajsaostm33FdvtWqW76NPR4ceDd9GQliEyQJfySOc5JdrxglvHjYmTxDpCXKZ1kQJ9AUbFVWyBVhhBHcu0YuABrVJpzJTJRcHuod2qrtwDhCFW0SmLnQA8J87wPBWcaRarrEhpLPIZernpHGkGzXKfOuNM9mrLVPrWk771Zuj5WLXphTr-VOnZpoclmJH_N6IqV3dL6XfJJAkHPjZD9_seHx8DDJd7YbwZ4hMsjBW8RPf0SlWeBqluwDpJUnjvlx4101tCgCUHVadTedbJpZPNb9O1siA2I8poSQptJZAtLcE7aZlwZyyxSoCJQSMFfP39s-XB_AP1Bw__veojdJ36qYWwQP0x2l21nXuCrpnzVblsnwZTg5IvBJTH3w6h_Px-_gvsHjSq |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZKQYILD_FangZx4BKa2E7sIC5VoSqiXVWoSBUXy6_QSFUSZTegvfET-I38EsZONktUkJC4RYmdyPaM55vJ-BuEXnCSEptzF8XEgoMCDkOU85REPovNAULPmbGh2ASfz8XpaX68hd6sz8L0_BBjwM1rRtivvYL7gPTOhjXUVC155Y9-XkKXGVgZX7-AsONNhEVklATWXRBLGgnKxJpaKCY7Y--pQbqAMi8mSw6UolM0G8zR_o3_G8hNdH2AoXi3l5tbaMtVt9E3kBl8VFblz-8_whnN-jXexa1qSos9rTHcHsr6NN0S07cYNtNA3OFwKA5VL1dNaTDAcbXCAIZxXQVO7BWGvQn8ZbCSWFUW27b7gu0mW-kO-rT_7mTvIBoKM0QGAFMe6TwniXa84NZxY2LGrEsSl2ldpABgAFNxFVsAFkYQRzOtKNhAq1QaU2VyUdC7aLuqK3cfYfBWdEpjJ0CimTM8TwWnmsYqK1ISi3yGXq5XR5qBtdwXzziXPd8ykX72pJ-9GXo-Nm16qo4_NXq2XmIJiuT_jqjK1d1C-nMyjHFAZDN0r1_y8TWAcbmnxpshPhGGsYEn6Z4-qcqzQNYN0p6wFL77uRebaZfgYsmB1-lMnndy4WTzW8BWFonNEmW0TAptJcAtLcE-acm4MzaxSoCSwSQFAfv7sOXe_CPxFw_-velTdPXg5OhQHr6ff3iIrhEfaAjp6o_Q9rLt3GN0xXxdlov2SdC7X0poNWQ |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1fi9QwEA_nKeLLqajn-jeKD77Ua5O0acSX485FUZdFFA5fQv7VKxxt2d2e7Jsfwc_oJ3GSdruWUxB8K23SkmQm85tp5jcIPeMkJVZwF8XEgoMCDkMkeEoif4rNAUIXzNhQbILPZvnJiZjvoFebXJiOH2IIuHnNCPu1V3DX2OJgyxpqqgV54VM_L6HLLIU91vM6s_k2wpJnlATWXRBLGuWU5RtqoZgcDL3HBukCyrx4WLKnFB2j2WCOptf_byA30F4PQ_FhJzc30Y6rbqFvIDP4Q1mVP7__CDma9Ut8iBeqKS32tMZwuy_r07QrTI8xbKaBuMPhUByqXq2b0mCA42qNAQzjugqc2GsMexP4y2Alsaostov2K7bb00q30efp609Hb6K-MENkADCJSAtBEu14wa3jxsSMWZckLtO6SAHAAKbiKrYALExOHM20omADrVJpTJUReUHvoN2qrtxdhMFb0SmNXQ4SzZzhIs051TRWWZGSOBcT9HyzOtL0rOW-eMaZ7PiWifSzJ_3sTdDToWnTUXX8qdGTzRJLUCT_d0RVrm6X0ufJMMYBkU3Qfrfkw2sA43JPjTdBfCQMQwNP0j1-UpWngawbpD1hKXz3Syc24y7BxZI9r9OpPGvl0snmt4CtLBKbJcpomRTaSoBbWoJ90pJxZ2xiVQ5KBpMUBOzvw5ZHs4_EX9z796aP0dX58VS-fzt7dx9dIz7OEE6rP0C7q0XrHqIr5nxVLhePgtr9Aqr6NOg |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+Mini-Organo%3A+A+rapid+high-throughput+3D+coculture+organotypic+assay+for+oncology+screening+and+drug+development&rft.jtitle=Cancer+reports&rft.au=Chitty%2C+Jessica+L&rft.au=Skhinas%2C+Joanna+N&rft.au=Filipe%2C+Elysse+C&rft.au=Wang%2C+Shan&rft.date=2020-02-01&rft.issn=2573-8348&rft.eissn=2573-8348&rft.volume=3&rft.issue=1&rft.spage=e1209&rft_id=info:doi/10.1002%2Fcnr2.1209&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2573-8348&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2573-8348&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2573-8348&client=summon |