“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry

NAD(H)‐utiliing enzymes have been the subject of directed evolution campaigns to improve their function. To enable access to a larger swath of sequence space, we demonstrate the utility of a cell‐free, ultrahigh‐throughput directed evolution platform for dehydrogenases. Microbeads (1.5 million per s...

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Vydáno v:	Angewandte Chemie International Edition Ročník 60; číslo 16; s. 9015 - 9021
Hlavní autoři:	Lindenburg, Laurens, Hollfelder, Florian
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Germany Wiley Subscription Services, Inc 12.04.2021 John Wiley and Sons Inc
Vydání:	International ed. in English
Témata:	Beads Biological evolution cell-free expression Dehydrogenases Directed evolution Flow cytometry Fluorescence Formate dehydrogenase Libraries Microspheres Modular design NAD NADH Nanoparticles Nicotinamide adenine dinucleotide Phenotypes Saturation saturation library Screening Substrates water-in-oil emulsion droplets cell-free expression formate dehydrogenase water-in-oil emulsion droplets saturation library directed evolution
ISSN:	1433-7851, 1521-3773, 1521-3773
On-line přístup:	Získat plný text
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