“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry

NAD(H)‐utiliing enzymes have been the subject of directed evolution campaigns to improve their function. To enable access to a larger swath of sequence space, we demonstrate the utility of a cell‐free, ultrahigh‐throughput directed evolution platform for dehydrogenases. Microbeads (1.5 million per s...

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Bibliographic Details
Published in:	Angewandte Chemie International Edition Vol. 60; no. 16; pp. 9015 - 9021
Main Authors:	Lindenburg, Laurens, Hollfelder, Florian
Format:	Journal Article
Language:	English
Published:	Germany Wiley Subscription Services, Inc 12.04.2021 John Wiley and Sons Inc
Edition:	International ed. in English
Subjects:	Beads Biological evolution cell-free expression Dehydrogenases Directed evolution Flow cytometry Fluorescence Formate dehydrogenase Libraries Microspheres Modular design NAD NADH Nanoparticles Nicotinamide adenine dinucleotide Phenotypes Saturation saturation library Screening Substrates water-in-oil emulsion droplets cell-free expression formate dehydrogenase water-in-oil emulsion droplets saturation library directed evolution
ISSN:	1433-7851, 1521-3773, 1521-3773
Online Access:	Get full text
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