Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from...

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Vydané v:	European journal of immunology Ročník 55; číslo 1; s. e2250325 - n/a
Hlavní autori:	Dudziak, Diana, Heger, Lukas, Agace, William W, Bakker, Joyce, Gruijl, Tanja D., Dress, Regine J., Dutertre, Charles‐Antoine, Fenton, Thomas M., Fransen, Marieke F., Ginhoux, Florent, Heyman, Oded, Horev, Yael, Hornsteiner, Florian, Kandiah, Vinitha, Kles, Paz, Lubin, Ruth, Mizraji, Gabriel, Prokopi, Anastasia, Saar, Or, Sopper, Sieghart, Stoitzner, Patrizia, Strandt, Helen, Sykora, Martina M, Toffoli, Elisa C., Tripp, Christoph H., Pul, Kim, Ven, Rieneke, Wilensky, Asaf, Yona, Simon, Zelle‐Rieser, Claudia
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Germany Wiley Subscription Services, Inc 01.01.2025 John Wiley and Sons Inc
Predmet:	Animals Basic Medicine Cell and Molecular Biology Cell suspensions Cell- och molekylärbiologi Dendritic cells Dendritic Cells - cytology Dendritic Cells - immunology Flow cytometry Flow Cytometry - methods Fluorescence microscopy Guidelines\|Clinical Highlights Humans Immunology Lymph nodes Lymphatic drainage Medical and Health Sciences Medicin och hälsovetenskap Medicinska och farmaceutiska grundvetenskaper Mice nonlymphoid tissues Phenotypes Single-Cell Analysis - methods tumor tumor‐draining lymph node nonlymphoid tissues flow cytometry dendritic cells tumor tumor‐draining lymph node
ISSN:	0014-2980, 1521-4141, 1521-4141
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Popis
Shrnutí:	This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single‐cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor‐draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single‐cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer‐reviewed by leading experts and approved by all co‐authors, making it an essential resource for basic and clinical DC immunologists. We here provide detailed protocols for isolating single cell suspension from human nonlymphoid tissues, such as gingiva, lung, intestine, skin, and different tumor tissues as well as tumor‐draining lymph nodes. Further, we provide gating strategies for flow cytometric analysis of human DC subpopulations in diverse nonlymphoid tissues.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0014-2980 1521-4141 1521-4141
DOI:	10.1002/eji.202250325