Quantitative Analysis of Porcine Endogenous Retroviruses in Different Organs of Transgenic Pigs Generated for Xenotransplantation

The pig appears to be the most promising animal donor of organs for use in human recipients. Among several types of pathogens found in pigs, one of the greatest problems is presented by porcine endogenous retroviruses (PERVs). Screening of the source pig herd for PERVs should include analysis of bot...

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Published in:Current microbiology Vol. 67; no. 4; pp. 505 - 514
Main Authors: Mazurek, Urszula, Kimsa, Magdalena C., Strzalka-Mrozik, Barbara, Kimsa, Malgorzata W., Adamska, Jolanta, Lipinski, Daniel, Zeyland, Joanna, Szalata, Marlena, Slomski, Ryszard, Jura, Jacek, Smorag, Zdzislaw, Nowak, Roman, Gola, Joanna
Format: Journal Article
Language:English
Published: Boston Springer US 01.10.2013
Springer Nature B.V
Subjects:
alpha-galactosidase
Animals
Animals, Genetically Modified
Animals, Genetically Modified - virology
Biomedical and Life Sciences
Biotechnology
Blood
Blood - virology
Cardiac muscle
classification
Deoxyribonucleic acid
DNA
Endogenous Retroviruses
Endogenous Retroviruses - classification
Endogenous Retroviruses - genetics
Endogenous Retroviruses - isolation & purification
Gene Dosage
genes
genetics
heart
Heart - virology
herds
Hogs
Humans
isolation & purification
Life Sciences
liver
Liver - virology
Microbiology
Muscles
Muscles - virology
Organs
pathogens
quantitative analysis
Retrovirus
Ribonucleic acid
risk
RNA
screening
Skin
Skin - virology
Swine
Swine - virology
Transgenic animals
Transplantation, Heterologous
Viral Proteins
Viral Proteins - genetics
virology
Viruses
Xenotransplantation
Porcine Tissue
Fucosyltransferase Gene
Xenograft Rejection
Human Recipient
Perv Infection
ISSN:0343-8651, 1432-0991, 1432-0991
Online Access:Get full text
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Summary:The pig appears to be the most promising animal donor of organs for use in human recipients. Among several types of pathogens found in pigs, one of the greatest problems is presented by porcine endogenous retroviruses (PERVs). Screening of the source pig herd for PERVs should include analysis of both PERV DNA and RNA. Therefore, the present study focuses on quantitative analysis of PERVs in different organs such as the skin, heart, muscle, and liver and blood of transgenic pigs generated for xenotransplantation. Transgenic pigs were developed to express the human α-galactosidase, the human α-1,2-fucosyltransferase gene, or both genetic modifications of the genome (Lipinski et al., Medycyna Wet 66:316–322, 2010 ; Lipinski et al., Ann Anim Sci 12:349–356, 2012 ; Wieczorek et al., Medycyna Wet 67:462–466, 2011 ). The copy numbers of PERV DNA and RNA were evaluated using real-time Q-PCR and QRT-PCR, respectively. Comparative analysis of all PERV subtypes revealed the following relationships: PERV A > PERV B > PERV C. PERV A and B were found in all samples, whereas PERV C was detected in 47 % of the tested animals. The lowest level of PERV DNA was shown in the muscles for PERV A and B and in blood samples for PERV C. The lowest level of PERV A RNA was found in the skin, whereas those of PERV B and C RNA were found in liver specimens. Quantitative analysis revealed differences in the copy number of PERV subtypes between various organs of transgenic pigs generated for xenotransplantation. Our data support the idea that careful pig selection for organ donation with low PERV copy number may limit the risk of retrovirus transmission to the human recipients.
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ISSN:0343-8651
1432-0991
1432-0991
DOI:10.1007/s00284-013-0397-3