Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix were sequenced. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts with known concentration. cDNA librar...

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Bibliographic Details
Published in:	Scientific reports Vol. 6; no. 1; p. 31602
Main Authors:	Oikonomopoulos, Spyros, Wang, Yu Chang, Djambazian, Haig, Badescu, Dunarel, Ragoussis, Jiannis
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 24.08.2016 Nature Publishing Group
Subjects:	38/23 38/39 38/91 45 45/23 45/91 631/1647/2017/2003 631/1647/2217/2018 631/208/514/1949 631/208/514/2254 Agreements Animals DNA, Complementary - genetics Gene expression Gene Library HEK293 Cells High-Throughput Nucleotide Sequencing - methods Humanities and Social Sciences Humans Isoforms multidisciplinary Nanopores Performance assessment Performance evaluation Polyadenylation Population Science
ISSN:	2045-2322, 2045-2322
Online Access:	Get full text
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