Discovering and validating between-subject variations in plasma lipids in healthy subjects

Lipid levels are commonly used in clinical settings as disease biomarkers and the advent of mass spectrometry-based (MS) lipidomics heralds the possibility of identifying additional lipids that can inform disease predispositions. However, the degree of natural variation for many lipids remains poorl...

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Vydáno v:	Scientific reports Ročník 6; číslo 1; s. 19139
Hlavní autoři:	Begum, Husna, Li, Bowen, Shui, Guanghou, Cazenave-Gassiot, Amaury, Soong, Richie, Ong, Rick Twee-Hee, Little, Peter, Teo, Yik-Ying, Wenk, Markus R.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 08.01.2016 Nature Publishing Group
Témata:	631/45/287/1194 692/308 Adult Biological variation Chromatography, High Pressure Liquid Female Healthy Volunteers Humanities and Social Sciences Humans Lecithin Lipid Metabolism - physiology Lipids Male Mass Spectrometry Mass spectroscopy multidisciplinary Observer Variation Phosphatidylcholine Phosphatidylcholines - blood Phosphatidylethanolamine Phosphatidylethanolamines - blood Pilot Projects Reference Values Reproducibility of Results Science Scientific imaging Singapore
ISSN:	2045-2322, 2045-2322
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Lipid levels are commonly used in clinical settings as disease biomarkers and the advent of mass spectrometry-based (MS) lipidomics heralds the possibility of identifying additional lipids that can inform disease predispositions. However, the degree of natural variation for many lipids remains poorly understood, thus confounding downstream investigations on whether a specific intervention is driving observed lipid fluctuations. Here, we performed targeted mass spectrometry with multiple reaction monitoring across a comprehensive spectrum of 192 plasma lipids on eight subjects across three time-points separated by six hours and two standardized meals. A validation study to confirm the initial discoveries was performed in a further set of nine subjects, subject to the identical study design. Technical variation of the MS was assessed using duplicate measurements in the validation study, while biological variation was measured for lipid species with coefficients of variation <20%. We observed that eight lipid species from the phosphatidylethanolamine and phosphatidylcholine lipid classes were discovered and validated to vary consistently across the three time-points, where the within-subject variance can be up to 1.3-fold higher than between-subject variance. These findings highlight the importance of understanding the range of biological variation in plasma lipids as a precursor to their use in clinical biochemistry.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Undefined-3 These authors contributed equally to this work.
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep19139