Digital Detection of Exosomes by Interferometric Imaging

Exosomes, which are membranous nanovesicles, are actively released by cells and have been attributed to roles in cell-cell communication, cancer metastasis, and early disease diagnostics. The small size (30–100 nm) along with low refractive index contrast of exosomes makes direct characterization an...

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Vydáno v:	Scientific reports Ročník 6; číslo 1; s. 37246
Hlavní autoři:	Daaboul, George G., Gagni, Paola, Benussi, Luisa, Bettotti, Paolo, Ciani, Miriam, Cretich, Marina, Freedman, David S., Ghidoni, Roberta, Ozkumur, Ayca Yalcin, Piotto, Chiara, Prosperi, Davide, Santini, Benedetta, Ünlü, M. Selim, Chiari, Marcella
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 17.11.2016 Nature Publishing Group
Témata:	631/61/32 692/53/2421 Alzheimer's disease Antibodies Cancer Cell culture Cell interactions Cell signaling Cerebrospinal fluid Cerebrospinal Fluid - chemistry Exosomes Exosomes - chemistry Genotype & phenotype HEK293 Cells Humanities and Social Sciences Humans Interferometry - methods Laboratories Metastases Microarray Analysis - instrumentation Microarray Analysis - methods multidisciplinary Nanoparticles Phenotyping Proteins Reagents Science Sensors
ISSN:	2045-2322, 2045-2322
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Exosomes, which are membranous nanovesicles, are actively released by cells and have been attributed to roles in cell-cell communication, cancer metastasis, and early disease diagnostics. The small size (30–100 nm) along with low refractive index contrast of exosomes makes direct characterization and phenotypical classification very difficult. In this work we present a method based on Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) that allows multiplexed phenotyping and digital counting of various populations of individual exosomes (>50 nm) captured on a microarray-based solid phase chip. We demonstrate these characterization concepts using purified exosomes from a HEK 293 cell culture. As a demonstration of clinical utility, we characterize exosomes directly from human cerebrospinal fluid (hCSF). Our interferometric imaging method could capture, from a very small hCSF volume (20 uL), nanoparticles that have a size compatible with exosomes, using antibodies directed against tetraspanins. With this unprecedented capability, we foresee revolutionary implications in the clinical field with improvements in diagnosis and stratification of patients affected by different disorders.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work.
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep37246