Isolation and Characterisation of Alongshan Virus in Russia

In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmente...

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Vydáno v:	Viruses Ročník 12; číslo 4; s. 362
Hlavní autoři:	Kholodilov, Ivan S., Litov, Alexander G., Klimentov, Alexander S., Belova, Oxana A., Polienko, Alexandra E., Nikitin, Nikolai A., Shchetinin, Alexey M., Ivannikova, Anna Y., Bell-Sakyi, Lesley, Yakovlev, Alexander S., Bugmyrin, Sergey V., Bespyatova, Liubov A., Gmyl, Larissa V., Luchinina, Svetlana V., Gmyl, Anatoly P., Gushchin, Vladimir A., Karganova, Galina G.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland MDPI AG 26.03.2020 MDPI
Témata:	adults alongshan virus Animals Cell Line cell lines Computational Biology - methods cytopathogenicity DNA sequencing flavi-like virus Flaviviridae Flaviviridae - classification Flaviviridae - genetics Flaviviridae - isolation & purification Flaviviridae - ultrastructure Flaviviridae Infections - epidemiology Flaviviridae Infections - transmission Flaviviridae Infections - virology flavivirus Flaviviruses Genetic aspects genome Genome, Viral Genomics - methods Geography, Medical High-Throughput Nucleotide Sequencing humans Identification and classification Ixodes persulcatus jingmen tick virus Methods Molecular Sequence Annotation open reading frame open reading frames phylogeny Russia Russia - epidemiology tick cell line ticks Ticks - virology Viral proteins virion viruses Russia Ixodes persulcatus Jingmen tick virus Alongshan virus flavi-like virus open reading frame tick cell line flavivirus
ISSN:	1999-4915, 1999-4915
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Popis
Shrnutí:	In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this article. Deceased.
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v12040362