Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant

Small animal models are of crucial importance for assessing COVID-19 countermeasures. Common laboratory mice would be well-suited for this purpose but are not susceptible to infection with wild-type SARS-CoV-2. However, the development of mouse-adapted virus strains has revealed key mutations in the...

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Veröffentlicht in:	Viruses Jg. 13; H. 11; S. 2263
Hauptverfasser:	Kant, Ravi, Kareinen, Lauri, Smura, Teemu, Freitag, Tobias L., Jha, Sawan Kumar, Alitalo, Kari, Meri, Seppo, Sironen, Tarja, Saksela, Kalle, Strandin, Tomas, Kipar, Anja, Vapalahti, Olli
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Switzerland MDPI AG 11.11.2021 MDPI
Schlagworte:	Animal models Animals Antibodies Antiviral agents Antiviral drugs Brain - virology COVID-19 COVID-19 - pathology COVID-19 - virology COVID-19 infection Disease Models, Animal disease susceptibility Female Infections Infectivity Inflammation Laboratories Laboratory animals laboratory mice Lung - pathology Lung - virology Lungs Male Mice Mice, Inbred BALB C Mutation Nose - virology pathogenicity Pulmonary Alveoli - pathology RNA polymerase SARS-CoV-2 - isolation & purification SARS-CoV-2 beta variants SARS-CoV-2 variants SARS-CoV2 Severe acute respiratory syndrome coronavirus 2 Spike protein Viruses St Louis Missouri United States > US COVID-19 SARS-CoV2 SARS-CoV-2 variants infections laboratory mice common laboratory mice SARS-CoV-2 beta variants
ISSN:	1999-4915, 1999-4915
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Zusammenfassung:	Small animal models are of crucial importance for assessing COVID-19 countermeasures. Common laboratory mice would be well-suited for this purpose but are not susceptible to infection with wild-type SARS-CoV-2. However, the development of mouse-adapted virus strains has revealed key mutations in the SARS-CoV-2 spike protein that increase infectivity, and interestingly, many of these mutations are also present in naturally occurring SARS-CoV-2 variants of concern. This suggests that these variants might have the ability to infect common laboratory mice. Herein we show that the SARS-CoV-2 beta variant attains infectibility to BALB/c mice and causes pulmonary changes within 2–3 days post infection, consistent with results seen in other murine models of COVID-19, at a reasonable virus dose (2 × 105 PFU). The findings suggest that common laboratory mice can serve as the animal model of choice for testing the effectiveness of antiviral drugs and vaccines against SARS-CoV-2.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work.
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v13112263