Production of high‐titer transmission‐defective RNA virus‐based episomal vector using tangential flow filtration

In recent years, viral vector based in vivo gene delivery strategies have achieved a significant success in the treatment of genetic diseases. RNA virus‐based episomal vector lacking viral glycoprotein gene (ΔG‐REVec) is a nontransmissive gene delivery system that enables long‐term gene expression i...

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Bibliographic Details
Published in:	Microbiology and immunology Vol. 64; no. 9; pp. 602 - 609
Main Authors:	Komatsu, Yumiko, Kakuya, Yoji, Tomonaga, Keizo
Format:	Journal Article
Language:	English
Published:	Australia Wiley Subscription Services, Inc 01.09.2020
Subjects:	Animals Brain - virology Cell Line Chlorocebus aethiops Female Filtration Filtration - methods Gene Expression Gene transfer Gene Transfer Techniques Genetic Vectors - administration & dosage Genetic Vectors - isolation & purification HEK293 Cells high‐titer Humans in vivo gene delivery Plasmids - genetics Plasmids - isolation & purification Pregnancy Rats Rats, Inbred Lew RNA viruses RNA Viruses - genetics RNA Viruses - isolation & purification RNA virus‐based episomal vector tangential flow filtration Transgenes Vero Cells Viral Load viral vector in vivo gene delivery viral vector RNA virus-based episomal vector tangential flow filtration high-titer
ISSN:	0385-5600, 1348-0421, 1348-0421
Online Access:	Get full text
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