Engineering and expressing circular RNAs via tRNA splicing

Circular (circ)RNAs have recently become a subject of great biologic interest. It is now clear that they represent a diverse and abundant class of RNAs with regulated expression and evolutionarily conserved functions. There are several mechanisms by which RNA circularization can occur in vivo. Here,...

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Veröffentlicht in:RNA biology Jg. 14; H. 8; S. 978 - 984
Hauptverfasser: Noto, John J., Schmidt, Casey A., Matera, A. Gregory
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Taylor & Francis 03.08.2017
Schlagworte:
Animals
Aptamers, Nucleotide - genetics
Aptamers, Nucleotide - metabolism
Archaea
Archaea - genetics
Archaea - metabolism
Back-splicing
biogenesis
circRNA
circular RNA
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
ectopic gene expression
Exons
Genetic Engineering - methods
HEK293 Cells
HeLa Cells
Humans
Introns
Mice
Point of Views
RNA - genetics
RNA - metabolism
RNA aptamers
RNA Precursors - genetics
RNA Precursors - metabolism
RNA Splice Sites
RNA Splicing
RNA, Transfer - genetics
RNA, Transfer - metabolism
Spliceosomes - metabolism
Spliceosomes - ultrastructure
transfer RNA
tricRNA
tRNA processing
RNA aptamers
ectopic gene expression
Back-splicing
circRNA
tRNA processing
tricRNA
ISSN:1547-6286, 1555-8584, 1555-8584
Online-Zugang:Volltext
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Zusammenfassung:Circular (circ)RNAs have recently become a subject of great biologic interest. It is now clear that they represent a diverse and abundant class of RNAs with regulated expression and evolutionarily conserved functions. There are several mechanisms by which RNA circularization can occur in vivo. Here, we focus on the biogenesis of tRNA intronic circular RNAs (tricRNAs) in archaea and animals, and we detail their use as research tools for orthogonal, directed circRNA expression in vivo.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1547-6286
1555-8584
1555-8584
DOI:10.1080/15476286.2017.1317911