Evaluation of IP-10 in Quantiferon-Plus as biomarker for the diagnosis of latent tuberculosis infection

The QuantiFERON-TB Gold Plus (QFT-Plus) is a new test for latent tuberculosis infection (LTBI) diagnosis, in which has been added a new tube containing shorter peptides stimulating CD8 T-cells and CD4-stimulating-peptides. Measurement of alternative biomarkers to Interferon-γ (IFN-γ) in QFT-Plus may...

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Vydané v:Tuberculosis (Edinburgh, Scotland) Ročník 111; s. 147 - 153
Hlavní autori: Petrone, Linda, Vanini, Valentina, Chiacchio, Teresa, Petruccioli, Elisa, Cuzzi, Gilda, Schininà, Vincenzo, Palmieri, Fabrizio, Ippolito, Giuseppe, Goletti, Delia
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Scotland Elsevier Ltd 01.07.2018
Elsevier Science Ltd
Predmet:
Adult
Bacterial Load
Biomarkers
Biomarkers - blood
Case-Control Studies
Chemokine CXCL10 - blood
Chemokine CXCL10 - immunology
Diagnosis
Enzyme-Linked Immunospot Assay - methods
Female
Host-Pathogen Interactions
Humans
Inflammation
Interferon
Interferon-gamma - blood
Interferon-gamma - immunology
Interferon-gamma Release Tests - methods
IP-10
Latent Tuberculosis - blood
Latent Tuberculosis - diagnosis
Latent Tuberculosis - immunology
Latent Tuberculosis - microbiology
LTBI
Male
Medical diagnosis
Middle Aged
Mycobacterium tuberculosis - immunology
Mycobacterium tuberculosis - pathogenicity
Peptides
Predictive Value of Tests
Quantiferon-plus
Reproducibility of Results
Sensitivity
Tuberculosis
Tubes
Quantiferon-plus
Inflammation
Tuberculosis
Diagnosis
IP-10
LTBI
ISSN:1472-9792, 1873-281X, 1873-281X
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Shrnutí:The QuantiFERON-TB Gold Plus (QFT-Plus) is a new test for latent tuberculosis infection (LTBI) diagnosis, in which has been added a new tube containing shorter peptides stimulating CD8 T-cells and CD4-stimulating-peptides. Measurement of alternative biomarkers to Interferon-γ (IFN-γ) in QFT-Plus may improve its sensitivity. Interferon-γ inducible protein 10 (IP-10), has been proposed as a tuberculosis (TB) biomarker. We aimed to evaluate the IP-10 accuracy in QFT-Plus for LTBI diagnosis. QFT-Plus was performed in 36 active TB, 31 LTBI and 16 healthy donors (HD). IP-10 was detected by ELISA. IP-10 is increased in TB1 and TB2 tubes in subjects with active TB and LTBI compared to HD. A ROC analysis comparing active TB and HD was performed and a cut-off of 1174 pg/mL for TB1 and 928.8 pg/mL for TB2 identified active TB with 86% sensitivity (Se) and 94% specificity (Sp). Moreover, increased IP-10 in response to TB1 was found in subjects with LTBI compared to those with active TB. A cut-off point of ≥16,108 pg/mL was chosen to maximize the test performance. However, the test predicted LTBI only with 58% Se and 61% Sp. These results suggest that IP-10 is an alternative biomarker to IFN-γ in the QFT-Plus format.
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ISSN:1472-9792
1873-281X
1873-281X
DOI:10.1016/j.tube.2018.06.005