Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy
We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering...
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| Vydáno v: | Journal of biomedical optics Ročník 19; číslo 11; s. 111612 |
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| Hlavní autoři: | , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
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United States
01.11.2014
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| ISSN: | 1560-2281, 1560-2281 |
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| Abstract | We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation endproducts (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front. |
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| AbstractList | We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation endproducts (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front.We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation endproducts (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front. We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation endproducts (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front. |
| Author | Sulé-Suso, Josep Eklouh-Molinier, Christophe Wehbe, Katia Jeannesson, Pierre Cinque, Gianfelice Yang, Ying Guilbert, Marie Sockalingum, Ganesh D |
| Author_xml | – sequence: 1 givenname: Marie surname: Guilbert fullname: Guilbert, Marie organization: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France – sequence: 2 givenname: Christophe surname: Eklouh-Molinier fullname: Eklouh-Molinier, Christophe organization: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France – sequence: 3 givenname: Katia surname: Wehbe fullname: Wehbe, Katia organization: Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom – sequence: 4 givenname: Josep surname: Sulé-Suso fullname: Sulé-Suso, Josep organization: Keele University, Institute for Science and Technology in Medicine, Stoke-on-Trent, ST4 7QB, United KingdomeUniversity Hospital of North Staffordshire, Cancer Centre, Newcastle Road, Stoke-on-Trent ST4 6QG, United Kingdom – sequence: 5 givenname: Ying surname: Yang fullname: Yang, Ying organization: Keele University, Institute for Science and Technology in Medicine, Stoke-on-Trent, ST4 7QB, United Kingdom – sequence: 6 givenname: Gianfelice surname: Cinque fullname: Cinque, Gianfelice organization: Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom – sequence: 7 givenname: Pierre surname: Jeannesson fullname: Jeannesson, Pierre organization: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France – sequence: 8 givenname: Ganesh D surname: Sockalingum fullname: Sockalingum, Ganesh D organization: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France |
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| SubjectTerms | Algorithms Animals Biomarkers, Tumor - chemistry Biomarkers, Tumor - metabolism Cell Line, Tumor Cluster Analysis Collagen Type I - chemistry Collagen Type I - metabolism Neoplasms - chemistry Neoplasms - metabolism Rats Rats, Sprague-Dawley Single-Cell Analysis - methods Spectroscopy, Fourier Transform Infrared - methods Synchrotrons |
| Title | Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy |
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