Germacrone inhibits early stages of influenza virus infection

•Germacrone is a major component of the essential oil extracted from Rhizoma Curcuma.•Germacrone has antiviral effect against several influenza viruses dose-dependently.•The early stages of the viral life cycle are affected by germacrone.•Germacrone showed an effective protection of mice from lethal...

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Vydané v:	Antiviral research Ročník 100; číslo 3; s. 578 - 588
Hlavní autori:	Liao, Qingjiao, Qian, Zhengxu, Liu, Rui, An, Liwei, Chen, Xulin
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Kidlington Elsevier B.V 01.12.2013 Elsevier
Predmet:	Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral agents Antiviral Agents - administration & dosage Antiviral Agents - pharmacology Antiviral Agents - therapeutic use Biological and medical sciences Cell Line Chick Embryo Curcuma Dogs Dose-Response Relationship, Drug Drug Evaluation, Preclinical Drug Therapy, Combination Drugs, Chinese Herbal - administration & dosage Drugs, Chinese Herbal - pharmacology Drugs, Chinese Herbal - therapeutic use Germacrone Humans Infectious diseases Influenza A Virus, H1N1 Subtype - drug effects Influenza A Virus, H1N1 Subtype - physiology Influenza A Virus, H3N2 Subtype - drug effects Influenza A Virus, H3N2 Subtype - physiology Influenza B virus Influenza B virus - drug effects Influenza B virus - physiology Influenza virus Inhibitor Lung - virology Madin Darby Canine Kidney Cells Medical sciences Mice Orthomyxoviridae Infections - drug therapy Orthomyxoviridae Infections - prevention & control Oseltamivir - administration & dosage Oseltamivir - pharmacology Oseltamivir - therapeutic use Pharmacology. Drug treatments Phytotherapy Ribavirin - pharmacology Sesquiterpenes, Germacrane - administration & dosage Sesquiterpenes, Germacrane - pharmacology Sesquiterpenes, Germacrane - therapeutic use Specific Pathogen-Free Organisms Viral diseases Virus Attachment - drug effects Virus Cultivation Virus Replication - drug effects Influenza virus Germacrone Inhibitor Infection Virus Viral disease Orthomyxoviridae Early stage Influenzavirus
ISSN:	0166-3542, 1872-9096, 1872-9096
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Shrnutí:	•Germacrone is a major component of the essential oil extracted from Rhizoma Curcuma.•Germacrone has antiviral effect against several influenza viruses dose-dependently.•The early stages of the viral life cycle are affected by germacrone.•Germacrone showed an effective protection of mice from lethal infection of influenza. Highly pathogenic influenza viruses pose a serious public health threat to humans. Although vaccines are available, antivirals are needed to efficiently control disease progression and virus transmission due to the emergence of drug-resistant viral strains. In this study, germacrone, which is a major component of the essential oils extracted from Rhizoma Curcuma, was found to inhibit influenza virus replication. Germacrone showed antiviral activity against the H1N1 and H3N2 influenza A viruses and the influenza B virus in a dose-dependent manner. The viral protein expression, RNA synthesis and the production of infectious progeny viruses were decreased both in MDCK and A549 cells treated with germacrone. In a time-of-addition study, germacrone was found to exhibit an inhibitory effect on both the attachment/entry step and the early stages of the viral replication cycle. Germacrone also exhibited an effective protection of mice from lethal infection and reduced the virus titres in the lung. Furthermore, the combination of germacrone and oseltamivir exhibited an additive effect on the inhibition of influenza virus infection, both in vitro and in vivo. Our results suggest that germacrone may have the potential to be developed as a therapeutic agent alone or in combination with other agents for the treatment of influenza virus infection.
Bibliografia:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0166-3542 1872-9096 1872-9096
DOI:	10.1016/j.antiviral.2013.09.021