The Caenorhabditis elegans Transgenic Toolbox
The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, ne...
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| Published in: | Genetics (Austin) Vol. 212; no. 4; pp. 959 - 990 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
Genetics Society of America
01.08.2019
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| Subjects: | |
| ISSN: | 1943-2631, 0016-6731, 1943-2631 |
| Online Access: | Get full text |
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| Abstract | The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression. |
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| AbstractList | The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression. The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression.The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression. The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made very useful for understanding, , developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in , with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression. |
| Author | Nance, Jeremy Frøkjær-Jensen, Christian |
| Author_xml | – sequence: 1 givenname: Jeremy surname: Nance fullname: Nance, Jeremy organization: Helen L. and Martin S. Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York 10016 – sequence: 2 givenname: Christian surname: Frøkjær-Jensen fullname: Frøkjær-Jensen, Christian organization: King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), KAUST Environmental Epigenetics Program (KEEP), Thuwal 23955-6900, Saudi Arabia |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31405997$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright © 2019 by the Genetics Society of America. Copyright Genetics Society of America Aug 2019 Copyright © 2019 by the Genetics Society of America 2019 |
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| Keywords | transposon C. elegans transgenesis CRISPR/Cas9 recombinases WormBook genetic engineering |
| Language | English |
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| SubjectTerms | Aging Animals Animals, Genetically Modified Artificial chromosomes BASIC (programming language) Biology Caenorhabditis elegans Caenorhabditis elegans - genetics Cloning Control methods CRISPR CRISPR-Cas Systems Deactivation Gene expression Genetic engineering Genetic modification Genetic Techniques Genetics Genome editing Genomes Genomics Inactivation Laboratories Nematodes Nervous system Neurosciences Proteins Vectors (Biology) Wormbook Worms |
| Title | The Caenorhabditis elegans Transgenic Toolbox |
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