Novel Affinity Disks for Bovine Serum Albumin Purification

The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N′,N′-tetramethylene...

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Vydané v:	Applied biochemistry and biotechnology Ročník 175; číslo 1; s. 454 - 468
Hlavní autori:	Tuzmen, Nalan, Kalburcu, Tülden, Uygun, Deniz Aktaş, Akgol, Sinan, Denizli, Adil
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Boston Springer-Verlag 01.01.2015 Springer US Springer Nature B.V
Predmet:	Adsorption Ammonium Animals Biochemistry Biotechnology Blood bovine serum albumin Cattle Chemistry Chemistry and Materials Science Cryogels - chemistry Electrophoresis, Polyacrylamide Gel enthalpy Hydrogels ice ionic strength polyacrylamide gel electrophoresis Polymerization Proteins purification methods Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - isolation & purification Sodium chloride sodium dodecyl sulfate sorption isotherms temperature Bovine serum albumin Affinity adsorption Cryogels Dye ligand Blood
ISSN:	0273-2289, 1559-0291, 1559-0291
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Abstract	The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N′,N′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as −58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process.
AbstractList	The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as -58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process. The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N , N , N ′, N ′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as −58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process. The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N′,N′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as −58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process. The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N′,N′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as −58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process. The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as -58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process.The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as -58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process.
Author	Uygun, Deniz Aktaş Kalburcu, Tülden Denizli, Adil Tuzmen, Nalan Akgol, Sinan
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/25308615$$D View this record in MEDLINE/PubMed
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Cites_doi	10.1021/ac0105437 10.1016/j.ijbiomac.2006.01.011 10.1016/S1389-1723(03)80118-4 10.1021/ja02268a002 10.1016/j.chroma.2008.02.110 10.1002/jbm.a.32127 10.1081/SS-120003502 10.1016/S0165-022X(01)00209-3 10.1016/j.procbio.2005.03.069 10.1016/S0223-5234(02)01449-6 10.1016/j.tibtech.2003.08.002 10.1007/BF02347403 10.1016/j.chroma.2006.05.089 10.1002/mabi.200300040 10.1016/j.colsurfa.2006.08.046 10.1016/j.jhazmat.2008.07.132 10.1016/S0021-9673(02)01114-7 10.1016/j.colsurfa.2008.02.010 10.1002/pmic.200300397 10.1016/j.colsurfa.2007.04.087 10.1073/pnas.0506440102 10.1016/S0021-9673(02)01871-X 10.1021/j100842a045 10.1016/j.powtec.2011.03.039 10.1016/j.molstruc.2006.10.058 10.1016/j.colsurfa.2009.08.023 10.1002/app.26187 10.1016/0021-9673(92)80117-D 10.1023/A:1015396825274 10.1002/pmic.200300399 10.1023/A:1016386902611 10.1046/j.1526-0968.2001.00388.x 10.1016/0142-9612(95)99697-K 10.1007/BF02347316
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References	HidayatCNakajimaMTakagiMYoshidaTJ Biosci Bioeng2003951331381:CAS:528:DC%2BD3sXjt1Witbc%3D10.1016/S1389-1723(03)80118-4 GlanzelMBultmannRStarkeKFrahmAWEur J Med Chem2003383033121:CAS:528:DC%2BD3sXitlKisrk%3D10.1016/S0223-5234(02)01449-6 TemkinMIPyzhevVActa Physiochim. URSS1940123273561:CAS:528:DyaH3MXkslSmtw%3D%3D KopacTBozgeyikKYenerJColloids Surf A: Physicochem Eng Aspects200832219281:CAS:528:DC%2BD1cXmt1OqtLw%3D10.1016/j.colsurfa.2008.02.010 HanJSunGShaoHChenSBaoBJ Radioanal Nucl Chem19992428218241:CAS:528:DC%2BD3cXhs1SjsLk%3D10.1007/BF02347403 JiangCLiuRShenXZhuLSongFPowder Technol201121190941:CAS:528:DC%2BC3MXmslyhsrc%3D10.1016/j.powtec.2011.03.039 BirchRMO’ByrneCBoothIRCashPProteomic200337647761:CAS:528:DC%2BD3sXjvFGisbo%3D10.1002/pmic.200300397 MitznerSRKlammtSPeszynskiPHicksteinHKortenGStangeJSchmidtRTher Apher Dial2001554174221:CAS:528:DC%2BD38XlvFCjuw%3D%3D10.1046/j.1526-0968.2001.00388.x Martin, L. (2004) IVECCS Proceedings, 274–278. JungYKimSParkSJKimJMColloids Surf A: Physicochem Eng Aspects2008313–31416216610.1016/j.colsurfa.2007.04.087 DenizliAPiskinEJ Biochem Biophys Methods2001493914161:CAS:528:DC%2BD3MXnvFCmtLo%3D10.1016/S0165-022X(01)00209-3 LangmuirIJ Am Chem Soc191638222122951:CAS:528:DyaC28Xhs1egsQ%3D%3D10.1021/ja02268a002 ChuangVTKragh-HansenUOtagiriMPharmaceut Res2002195697710.1023/A:1015396825274 LiWLiSColloids Surf A: Physicochem Eng Aspects20072951591641:CAS:528:DC%2BD2sXhtlals7o%3D10.1016/j.colsurfa.2006.08.046 FreundlichHMFJ Phys Chem1906573854701:CAS:528:DyaD28XhtVCl LozinskyVIPlievaFMGalaevYIMattiassonBBioseparation2001101631881:CAS:528:DC%2BD38Xns1Kls7g%3D10.1023/A:1016386902611 NevensJRMalliaAKWendtMWSmithPKJ Chromatogr19925972472561:CAS:528:DyaK38Xitlegtbk%3D10.1016/0021-9673(92)80117-D ZhangYZZhouBZhangXPHuangPLiCHLiuYJ Hazard Mater2009163134513521:CAS:528:DC%2BD1MXhvFaitr0%3D10.1016/j.jhazmat.2008.07.132 SulkowskaAMaciazekMRownickaJBojkoBPentakDASulkowskiWWJ Mol Struc2007834–83616216910.1016/j.molstruc.2006.10.058 SimardJRZunszainPAHaCEYangJSBhagavanNVPetitpasICurrySHamiltonJAProc Natl Acad Sci U S A2005102179581:CAS:528:DC%2BD2MXhtlersr%2FJ10.1073/pnas.0506440102 TrivediVDSaxenaISiddiquiMUQasimMABiochem Mol Biol Int199743181:CAS:528:DyaK2sXnt12ksb4%3D Yan-MinLYan-ZhaoYXi-DanZChuan-BoXFood bioprodProcess2010884046 BereliNAndaçMBaydemirGSayRGalaevIYDenizliAJ Chromatogr A2008119018261:CAS:528:DC%2BD1cXkvFGiurw%3D10.1016/j.chroma.2008.02.110 AltıntasEBDenizliAInt J Biol Macromol2006389910610.1016/j.ijbiomac.2006.01.011 WolmanFJGraselliMCasconeOProcess Biochem2006413563611:CAS:528:DC%2BD2MXhtlGisLrP10.1016/j.procbio.2005.03.069 DubininMMRadushkevichLVProc Acad Sci USSR Phys Chem Sect194755331333 HanJSilcockPMcQuillanAJBremerPColloids Surf A: Physicochem Eng Aspects20093492072131:CAS:528:DC%2BD1MXhtFGhsbzK10.1016/j.colsurfa.2009.08.023 SmithJMChemical Engineering Kinetics1981NYMcGraw-Hill LozinskyVIGalaevIYPlievaFMSavinaINJungvidHAMattiassonBTrends Biotechnol2003214454511:CAS:528:DC%2BD3sXnsVKnsrY%3D10.1016/j.tibtech.2003.08.002 WassellDTHHallRCEmberyGBiomaterials1995166977021:CAS:528:DyaK2MXmsFWrsL8%3D10.1016/0142-9612(95)99697-K ArvidssonPPlievaFMLozinskyVIGalaevIYMattiassonBJ Chromatogr A20039862752901:CAS:528:DC%2BD3sXkvV2mtA%3D%3D10.1016/S0021-9673(02)01871-X TripathiAKathuriaNKumarAJ Biomed Mater Res A200990A6806941:CAS:528:DC%2BD1MXptVamsrk%3D10.1002/jbm.a.32127 ArvidssonPPlievaFMSavinaINLozinskyVIFexbySBulowLGalaevIYMattiassonBJ Chromatogr A200297727381:CAS:528:DC%2BD38Xotlyrsbw%3D10.1016/S0021-9673(02)01114-7 HanJSunGFangJBaoBJ Radioanal Nucl Chem19992412152171:CAS:528:DyaK1MXmtVeqs7o%3D10.1007/BF02347316 SteelLFShumpertDTrotterMGSeeholzerSHEvansAALondonWTProteomics200336016091:CAS:528:DC%2BD3sXjvFGisL4%3D10.1002/pmic.200300399 HermansonGTMalliaAKSmithPKIn Immobilized Affinity Ligand Techniques1992CaliforniaAcademic Pres Inc YavuzHDenizliAMacromol Biosci2004484911:CAS:528:DC%2BD2cXhvVKrurw%3D10.1002/mabi.200300040 DengQGermanIBuchananDKennedyRTAnal Chem200173541554211:CAS:528:DC%2BD3MXnsF2lu7s%3D10.1021/ac0105437 DainiakMBGalaevIYMatiassonBJ Chromatogr A200611231451501:CAS:528:DC%2BD28Xns12nsro%3D10.1016/j.chroma.2006.05.089 DemiryasNTuzmenNGalaevIYPiskinEDenizliAJ Appl Polym Sci2007105180818161:CAS:528:DC%2BD2sXntl2isbY%3D10.1002/app.26187 HobsonJPJ Phys Chem196973720272710.1021/j100842a045 YavuzHPatirSSayRAricaYDenizliASepar Sci Technol200237207720951:CAS:528:DC%2BD38Xlt1ajtbs%3D10.1081/SS-120003502 JP Hobson (1273_CR34) 1969; 73 YZ Zhang (1273_CR2) 2009; 163 1273_CR5 W Li (1273_CR28) 2007; 295 VI Lozinsky (1273_CR17) 2003; 21 Y Jung (1273_CR37) 2008; 313–314 VT Chuang (1273_CR3) 2002; 19 J Han (1273_CR27) 2009; 349 RM Birch (1273_CR12) 2003; 3 L Yan-Min (1273_CR6) 2010; 88 A Denizli (1273_CR16) 2001; 49 MM Dubinin (1273_CR33) 1947; 55 JM Smith (1273_CR35) 1981 J Han (1273_CR38) 1999; 242 T Kopac (1273_CR32) 2008; 322 LF Steel (1273_CR26) 2003; 3 A Tripathi (1273_CR23) 2009; 90A SR Mitzner (1273_CR4) 2001; 5 M Glanzel (1273_CR14) 2003; 38 MI Temkin (1273_CR31) 1940; 12 VI Lozinsky (1273_CR18) 2001; 10 DTH Wassell (1273_CR36) 1995; 16 EB Altıntas (1273_CR40) 2006; 38 JR Simard (1273_CR1) 2005; 102 JR Nevens (1273_CR8) 1992; 597 N Demiryas (1273_CR22) 2007; 105 Q Deng (1273_CR10) 2001; 73 H Yavuz (1273_CR15) 2004; 4 MB Dainiak (1273_CR21) 2006; 1123 H Yavuz (1273_CR9) 2002; 37 C Hidayat (1273_CR11) 2003; 95 HMF Freundlich (1273_CR30) 1906; 57 FJ Wolman (1273_CR13) 2006; 41 C Jiang (1273_CR42) 2011; 211 P Arvidsson (1273_CR19) 2003; 986 N Bereli (1273_CR41) 2008; 1190 GT Hermanson (1273_CR24) 1992 A Sulkowska (1273_CR7) 2007; 834–836 I Langmuir (1273_CR29) 1916; 38 VD Trivedi (1273_CR25) 1997; 43 P Arvidsson (1273_CR20) 2002; 977 J Han (1273_CR39) 1999; 241
References_xml	– reference: BirchRMO’ByrneCBoothIRCashPProteomic200337647761:CAS:528:DC%2BD3sXjvFGisbo%3D10.1002/pmic.200300397 – reference: KopacTBozgeyikKYenerJColloids Surf A: Physicochem Eng Aspects200832219281:CAS:528:DC%2BD1cXmt1OqtLw%3D10.1016/j.colsurfa.2008.02.010 – reference: JungYKimSParkSJKimJMColloids Surf A: Physicochem Eng Aspects2008313–31416216610.1016/j.colsurfa.2007.04.087 – reference: AltıntasEBDenizliAInt J Biol Macromol2006389910610.1016/j.ijbiomac.2006.01.011 – reference: MitznerSRKlammtSPeszynskiPHicksteinHKortenGStangeJSchmidtRTher Apher Dial2001554174221:CAS:528:DC%2BD38XlvFCjuw%3D%3D10.1046/j.1526-0968.2001.00388.x – reference: SulkowskaAMaciazekMRownickaJBojkoBPentakDASulkowskiWWJ Mol Struc2007834–83616216910.1016/j.molstruc.2006.10.058 – reference: FreundlichHMFJ Phys Chem1906573854701:CAS:528:DyaD28XhtVCl – reference: SimardJRZunszainPAHaCEYangJSBhagavanNVPetitpasICurrySHamiltonJAProc Natl Acad Sci U S A2005102179581:CAS:528:DC%2BD2MXhtlersr%2FJ10.1073/pnas.0506440102 – reference: YavuzHPatirSSayRAricaYDenizliASepar Sci Technol200237207720951:CAS:528:DC%2BD38Xlt1ajtbs%3D10.1081/SS-120003502 – reference: LangmuirIJ Am Chem Soc191638222122951:CAS:528:DyaC28Xhs1egsQ%3D%3D10.1021/ja02268a002 – reference: DemiryasNTuzmenNGalaevIYPiskinEDenizliAJ Appl Polym Sci2007105180818161:CAS:528:DC%2BD2sXntl2isbY%3D10.1002/app.26187 – reference: ArvidssonPPlievaFMLozinskyVIGalaevIYMattiassonBJ Chromatogr A20039862752901:CAS:528:DC%2BD3sXkvV2mtA%3D%3D10.1016/S0021-9673(02)01871-X – reference: LozinskyVIPlievaFMGalaevYIMattiassonBBioseparation2001101631881:CAS:528:DC%2BD38Xns1Kls7g%3D10.1023/A:1016386902611 – reference: HermansonGTMalliaAKSmithPKIn Immobilized Affinity Ligand Techniques1992CaliforniaAcademic Pres Inc – reference: WolmanFJGraselliMCasconeOProcess Biochem2006413563611:CAS:528:DC%2BD2MXhtlGisLrP10.1016/j.procbio.2005.03.069 – reference: TrivediVDSaxenaISiddiquiMUQasimMABiochem Mol Biol Int199743181:CAS:528:DyaK2sXnt12ksb4%3D – reference: ChuangVTKragh-HansenUOtagiriMPharmaceut Res2002195697710.1023/A:1015396825274 – reference: DengQGermanIBuchananDKennedyRTAnal Chem200173541554211:CAS:528:DC%2BD3MXnsF2lu7s%3D10.1021/ac0105437 – reference: LiWLiSColloids Surf A: Physicochem Eng Aspects20072951591641:CAS:528:DC%2BD2sXhtlals7o%3D10.1016/j.colsurfa.2006.08.046 – reference: DenizliAPiskinEJ Biochem Biophys Methods2001493914161:CAS:528:DC%2BD3MXnvFCmtLo%3D10.1016/S0165-022X(01)00209-3 – reference: DubininMMRadushkevichLVProc Acad Sci USSR Phys Chem Sect194755331333 – reference: YavuzHDenizliAMacromol Biosci2004484911:CAS:528:DC%2BD2cXhvVKrurw%3D10.1002/mabi.200300040 – reference: TripathiAKathuriaNKumarAJ Biomed Mater Res A200990A6806941:CAS:528:DC%2BD1MXptVamsrk%3D10.1002/jbm.a.32127 – reference: HanJSunGFangJBaoBJ Radioanal Nucl Chem19992412152171:CAS:528:DyaK1MXmtVeqs7o%3D10.1007/BF02347316 – reference: NevensJRMalliaAKWendtMWSmithPKJ Chromatogr19925972472561:CAS:528:DyaK38Xitlegtbk%3D10.1016/0021-9673(92)80117-D – reference: SmithJMChemical Engineering Kinetics1981NYMcGraw-Hill – reference: WassellDTHHallRCEmberyGBiomaterials1995166977021:CAS:528:DyaK2MXmsFWrsL8%3D10.1016/0142-9612(95)99697-K – reference: DainiakMBGalaevIYMatiassonBJ Chromatogr A200611231451501:CAS:528:DC%2BD28Xns12nsro%3D10.1016/j.chroma.2006.05.089 – reference: ArvidssonPPlievaFMSavinaINLozinskyVIFexbySBulowLGalaevIYMattiassonBJ Chromatogr A200297727381:CAS:528:DC%2BD38Xotlyrsbw%3D10.1016/S0021-9673(02)01114-7 – reference: Yan-MinLYan-ZhaoYXi-DanZChuan-BoXFood bioprodProcess2010884046 – reference: HanJSilcockPMcQuillanAJBremerPColloids Surf A: Physicochem Eng Aspects20093492072131:CAS:528:DC%2BD1MXhtFGhsbzK10.1016/j.colsurfa.2009.08.023 – reference: GlanzelMBultmannRStarkeKFrahmAWEur J Med Chem2003383033121:CAS:528:DC%2BD3sXitlKisrk%3D10.1016/S0223-5234(02)01449-6 – reference: ZhangYZZhouBZhangXPHuangPLiCHLiuYJ Hazard Mater2009163134513521:CAS:528:DC%2BD1MXhvFaitr0%3D10.1016/j.jhazmat.2008.07.132 – reference: HidayatCNakajimaMTakagiMYoshidaTJ Biosci Bioeng2003951331381:CAS:528:DC%2BD3sXjt1Witbc%3D10.1016/S1389-1723(03)80118-4 – reference: Martin, L. (2004) IVECCS Proceedings, 274–278. – reference: HobsonJPJ Phys Chem196973720272710.1021/j100842a045 – reference: BereliNAndaçMBaydemirGSayRGalaevIYDenizliAJ Chromatogr A2008119018261:CAS:528:DC%2BD1cXkvFGiurw%3D10.1016/j.chroma.2008.02.110 – reference: JiangCLiuRShenXZhuLSongFPowder Technol201121190941:CAS:528:DC%2BC3MXmslyhsrc%3D10.1016/j.powtec.2011.03.039 – reference: TemkinMIPyzhevVActa Physiochim. URSS1940123273561:CAS:528:DyaH3MXkslSmtw%3D%3D – reference: HanJSunGShaoHChenSBaoBJ Radioanal Nucl Chem19992428218241:CAS:528:DC%2BD3cXhs1SjsLk%3D10.1007/BF02347403 – reference: LozinskyVIGalaevIYPlievaFMSavinaINJungvidHAMattiassonBTrends Biotechnol2003214454511:CAS:528:DC%2BD3sXnsVKnsrY%3D10.1016/j.tibtech.2003.08.002 – reference: SteelLFShumpertDTrotterMGSeeholzerSHEvansAALondonWTProteomics200336016091:CAS:528:DC%2BD3sXjvFGisL4%3D10.1002/pmic.200300399 – volume: 73 start-page: 5415 year: 2001 ident: 1273_CR10 publication-title: Anal Chem doi: 10.1021/ac0105437 – volume: 38 start-page: 99 year: 2006 ident: 1273_CR40 publication-title: Int J Biol Macromol doi: 10.1016/j.ijbiomac.2006.01.011 – volume: 95 start-page: 133 year: 2003 ident: 1273_CR11 publication-title: J Biosci Bioeng doi: 10.1016/S1389-1723(03)80118-4 – volume: 38 start-page: 2221 year: 1916 ident: 1273_CR29 publication-title: J Am Chem Soc doi: 10.1021/ja02268a002 – volume: 1190 start-page: 18 year: 2008 ident: 1273_CR41 publication-title: J Chromatogr A doi: 10.1016/j.chroma.2008.02.110 – volume: 90A start-page: 680 year: 2009 ident: 1273_CR23 publication-title: J Biomed Mater Res A doi: 10.1002/jbm.a.32127 – volume: 37 start-page: 2077 year: 2002 ident: 1273_CR9 publication-title: Separ Sci Technol doi: 10.1081/SS-120003502 – volume: 49 start-page: 391 year: 2001 ident: 1273_CR16 publication-title: J Biochem Biophys Methods doi: 10.1016/S0165-022X(01)00209-3 – volume: 41 start-page: 356 year: 2006 ident: 1273_CR13 publication-title: Process Biochem doi: 10.1016/j.procbio.2005.03.069 – volume: 38 start-page: 303 year: 2003 ident: 1273_CR14 publication-title: Eur J Med Chem doi: 10.1016/S0223-5234(02)01449-6 – volume: 21 start-page: 445 year: 2003 ident: 1273_CR17 publication-title: Trends Biotechnol doi: 10.1016/j.tibtech.2003.08.002 – volume: 43 start-page: 1 year: 1997 ident: 1273_CR25 publication-title: Biochem Mol Biol Int – volume: 55 start-page: 331 year: 1947 ident: 1273_CR33 publication-title: Proc Acad Sci USSR Phys Chem Sect – volume: 242 start-page: 821 year: 1999 ident: 1273_CR38 publication-title: J Radioanal Nucl Chem doi: 10.1007/BF02347403 – ident: 1273_CR5 – volume: 1123 start-page: 145 year: 2006 ident: 1273_CR21 publication-title: J Chromatogr A doi: 10.1016/j.chroma.2006.05.089 – volume: 4 start-page: 84 year: 2004 ident: 1273_CR15 publication-title: Macromol Biosci doi: 10.1002/mabi.200300040 – volume-title: Chemical Engineering Kinetics year: 1981 ident: 1273_CR35 – volume: 295 start-page: 159 year: 2007 ident: 1273_CR28 publication-title: Colloids Surf A: Physicochem Eng Aspects doi: 10.1016/j.colsurfa.2006.08.046 – volume: 163 start-page: 1345 year: 2009 ident: 1273_CR2 publication-title: J Hazard Mater doi: 10.1016/j.jhazmat.2008.07.132 – volume: 977 start-page: 27 year: 2002 ident: 1273_CR20 publication-title: J Chromatogr A doi: 10.1016/S0021-9673(02)01114-7 – volume: 322 start-page: 19 year: 2008 ident: 1273_CR32 publication-title: Colloids Surf A: Physicochem Eng Aspects doi: 10.1016/j.colsurfa.2008.02.010 – volume: 57 start-page: 385 year: 1906 ident: 1273_CR30 publication-title: J Phys Chem – volume: 3 start-page: 764 year: 2003 ident: 1273_CR12 publication-title: Proteomic doi: 10.1002/pmic.200300397 – volume: 313–314 start-page: 162 year: 2008 ident: 1273_CR37 publication-title: Colloids Surf A: Physicochem Eng Aspects doi: 10.1016/j.colsurfa.2007.04.087 – volume: 102 start-page: 17958 year: 2005 ident: 1273_CR1 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0506440102 – volume: 986 start-page: 275 year: 2003 ident: 1273_CR19 publication-title: J Chromatogr A doi: 10.1016/S0021-9673(02)01871-X – volume: 73 start-page: 720 year: 1969 ident: 1273_CR34 publication-title: J Phys Chem doi: 10.1021/j100842a045 – volume: 211 start-page: 90 year: 2011 ident: 1273_CR42 publication-title: Powder Technol doi: 10.1016/j.powtec.2011.03.039 – volume: 834–836 start-page: 162 year: 2007 ident: 1273_CR7 publication-title: J Mol Struc doi: 10.1016/j.molstruc.2006.10.058 – volume: 349 start-page: 207 year: 2009 ident: 1273_CR27 publication-title: Colloids Surf A: Physicochem Eng Aspects doi: 10.1016/j.colsurfa.2009.08.023 – volume: 12 start-page: 327 year: 1940 ident: 1273_CR31 publication-title: Acta Physiochim. URSS – volume: 105 start-page: 1808 year: 2007 ident: 1273_CR22 publication-title: J Appl Polym Sci doi: 10.1002/app.26187 – volume: 597 start-page: 247 year: 1992 ident: 1273_CR8 publication-title: J Chromatogr doi: 10.1016/0021-9673(92)80117-D – volume: 19 start-page: 569 year: 2002 ident: 1273_CR3 publication-title: Pharmaceut Res doi: 10.1023/A:1015396825274 – volume: 3 start-page: 601 year: 2003 ident: 1273_CR26 publication-title: Proteomics doi: 10.1002/pmic.200300399 – volume: 10 start-page: 163 year: 2001 ident: 1273_CR18 publication-title: Bioseparation doi: 10.1023/A:1016386902611 – volume-title: In Immobilized Affinity Ligand Techniques year: 1992 ident: 1273_CR24 – volume: 5 start-page: 417 issue: 5 year: 2001 ident: 1273_CR4 publication-title: Ther Apher Dial doi: 10.1046/j.1526-0968.2001.00388.x – volume: 16 start-page: 697 year: 1995 ident: 1273_CR36 publication-title: Biomaterials doi: 10.1016/0142-9612(95)99697-K – volume: 241 start-page: 215 year: 1999 ident: 1273_CR39 publication-title: J Radioanal Nucl Chem doi: 10.1007/BF02347316 – volume: 88 start-page: 40 year: 2010 ident: 1273_CR6 publication-title: Process
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Snippet	The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel...
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SubjectTerms	Adsorption Ammonium Animals Biochemistry Biotechnology Blood bovine serum albumin Cattle Chemistry Chemistry and Materials Science Cryogels - chemistry Electrophoresis, Polyacrylamide Gel enthalpy Hydrogels ice ionic strength polyacrylamide gel electrophoresis Polymerization Proteins purification methods Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - isolation & purification Sodium chloride sodium dodecyl sulfate sorption isotherms temperature
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Title	Novel Affinity Disks for Bovine Serum Albumin Purification
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