Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa

In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. Rousettus aegyptiacus (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the...

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Vydané v:	Viruses Ročník 15; číslo 2; s. 498
Hlavní autori:	Rademan, Rochelle, Geldenhuys, Marike, Markotter, Wanda
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Switzerland MDPI AG 10.02.2023 MDPI
Predmet:	Adaptation Africa, Southern Amino Acids Animals Avian influenza viruses Bats birds Chiroptera Egyptian Rousette bat Feces genes Genomes H9N2 hosts Humans Identification and classification Influenza Influenza A Influenza A virus Influenza A Virus, H9N2 Subtype - genetics Influenza, Human Laboratories mammals monitoring Mutation RNA RNA viruses Rousettus Rousettus aegyptiacus Serology South Africa South Africa - epidemiology Surveillance Testing Virulence Viruses Zoonoses Africa, Southern South Africa Africa Johannesburg South Africa South Africa surveillance H9N2 Egyptian Rousette bat influenza A virus
ISSN:	1999-4915, 1999-4915
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Shrnutí:	In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. Rousettus aegyptiacus (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the molecular surveillance of a maternal colony in Limpopo, South Africa, between 2017–2018. A pan-influenza hemi-nested RT-PCR assay targeting the PB1 gene was established, and influenza A virus RNA was identified from one fecal sample out of 860 samples. Genome segments were recovered using segment-specific amplification combined with standard Sanger sequencing and Illumina unbiased sequencing. The identified influenza A virus was closely related to the H9N2 bat-influenza virus, confirming the circulation of this subtype among Egyptian fruit bat populations in Southern Africa. This bat H9N2 subtype contained amino acid residues associated with transmission and virulence in either mammalian or avian hosts, though it will likely require additional adaptations before spillover.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v15020498