Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution

Degradability is often a critical property of materials utilized in tissue engineering. Although alginate, a naturally derived polysaccharide, is an attractive material due to its biocompatibility and ability to form hydrogels, its slow and uncontrollable degradation can be an undesirable feature. I...

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Vydané v:Biomaterials Ročník 26; číslo 15; s. 2455 - 2465
Hlavní autori: Boontheekul, Tanyarut, Kong, Hyun-Joon, Mooney, David J.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Netherlands Elsevier Ltd 01.05.2005
Predmet:
Alginates - analysis
Alginates - chemistry
Animals
Biocompatibility
Biocompatible Materials - analysis
Biocompatible Materials - chemistry
Calcium - chemistry
Calcium cross-linking
Cell Adhesion - physiology
Cell Culture Techniques - methods
Cell Differentiation - physiology
Cell Line
Chain scission
Compressive Strength
Elasticity
Glucuronic Acid - analysis
Glucuronic Acid - chemistry
Hexuronic Acids - analysis
Hexuronic Acids - chemistry
Hydrogels - analysis
Hydrogels - chemistry
Materials Testing
Mice
Molecular Weight
Myoblasts
Myoblasts - cytology
Myoblasts - physiology
Oxidation-Reduction
Porosity
Tensile Strength
Tissue engineering
Tissue Engineering - methods
Chain scission
Biocompatibility
Tissue engineering
Calcium cross-linking
Myoblasts
ISSN:0142-9612, 1878-5905
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Shrnutí:Degradability is often a critical property of materials utilized in tissue engineering. Although alginate, a naturally derived polysaccharide, is an attractive material due to its biocompatibility and ability to form hydrogels, its slow and uncontrollable degradation can be an undesirable feature. In this study, we characterized gels formed using a combination of partial oxidation of polymer chains and a bimodal molecular weight distribution of polymer. Specifically, alginates were partially oxidized to a theoretical extent of 1% with sodium periodate, which created acetal groups susceptible to hydrolysis. The ratio of low MW to high MW alginates used to form gels was also varied, while maintaining the gel forming ability of the polymer. The rate of degradation was found to be controlled by both the oxidation and the ratio of high to low MW alginates, as monitored by the reduction of mechanical properties and corresponding number of crosslinks, dry weight loss, and molecular weight decrease. It was subsequently examined whether these modifications would lead to reduced biocompatibility by culturing C2C12 myoblast on these gels. Myoblasts adhered, proliferated, and differentiated on the modified gels at a comparable rate as those cultured on the unmodified gels. Altogether, this data indicates these hydrogels exhibit tunable degradation rates and provide a powerful material system for tissue engineering.
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ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2004.06.044