Capsid-dependent and -independent postentry restriction of primate lentivirus tropism in rodent cells

Retrovirus tropism can be restricted by cellular factors such as Fv1, Ref1, and Lv1 that inhibit infection by targeting the incoming viral capsid. Here, we show that rodent cells exhibit differential sensitivity to infection by vesicular stomatitis virus G-pseudotyped lentiviruses and that differenc...

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Bibliographic Details
Published in:	Journal of virology Vol. 78; no. 2; p. 1006
Main Authors:	Hatziioannou, Theodora, Cowan, Simone, Bieniasz, Paul D
Format:	Journal Article
Language:	English
Published:	United States 01.01.2004
Subjects:	Animals Capsid - metabolism Cell Line Green Fluorescent Proteins HIV-1 - pathogenicity HIV-1 - physiology HIV-2 - pathogenicity HIV-2 - physiology Humans Lentiviruses, Primate - pathogenicity Lentiviruses, Primate - physiology Luminescent Proteins - genetics Luminescent Proteins - metabolism Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Mice Mice, Inbred AKR Mice, Inbred C57BL NIH 3T3 Cells Proteins - metabolism Rodentia - virology Simian Immunodeficiency Virus - pathogenicity Simian Immunodeficiency Virus - physiology Viral Envelope Proteins - genetics Viral Envelope Proteins - metabolism
ISSN:	0022-538X
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Summary:	Retrovirus tropism can be restricted by cellular factors such as Fv1, Ref1, and Lv1 that inhibit infection by targeting the incoming viral capsid. Here, we show that rodent cells exhibit differential sensitivity to infection by vesicular stomatitis virus G-pseudotyped lentiviruses and that differences between human immunodeficiency virus type 1 and simian immunodeficiency virus (SIVmac) infectivity are sometimes, but not always, governed by determinants in capsid-p2. In at least one case, resistance to SIVmac infection could be eliminated by saturation of target cells with noninfectious SIVmac particles. However, cross-saturation experiments and analysis of Fv1-null cells engineered to express natural or artificial Fv1 proteins revealed that lentivirus restriction in mouse cells is independent of Fv1. Overall, these findings indicate that novel restriction factors in rodents can modulate sensitivity to specific primate lentiviruses.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-538X
DOI:	10.1128/JVI.78.2.1006-1011.2004