EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization

Determining the spatial organization and morphological characteristics of molecularly defined cell types is a major bottleneck for characterizing the architecture underpinning brain function. We developed Expansion-Assisted Iterative Fluorescence In Situ Hybridization (EASI-FISH) to survey gene expr...

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Bibliographic Details
Published in:	Cell Vol. 184; no. 26; p. 6361
Main Authors:	Wang, Yuhan, Eddison, Mark, Fleishman, Greg, Weigert, Martin, Xu, Shengjin, Wang, Tim, Rokicki, Konrad, Goina, Cristian, Henry, Fredrick E, Lemire, Andrew L, Schmidt, Uwe, Yang, Hui, Svoboda, Karel, Myers, Eugene W, Saalfeld, Stephan, Korff, Wyatt, Sternson, Scott M, Tillberg, Paul W
Format:	Journal Article
Language:	English
Published:	United States 22.12.2021
Subjects:	Animals Biomarkers - metabolism Gene Expression Profiling Gene Expression Regulation Hypothalamic Area, Lateral - cytology Hypothalamic Area, Lateral - metabolism Imaging, Three-Dimensional In Situ Hybridization, Fluorescence Male Mice Mice, Inbred C57BL Neurons - metabolism Neuropeptides - metabolism Proto-Oncogene Proteins c-fos - metabolism RNA - metabolism RNA-Seq Single-Cell Analysis Transcription, Genetic expansion microscopy RNA brain anatomy Fluorescence in situ hybridization lateral hypothalamus transcriptomics 3D segmentation cell type brain
ISSN:	1097-4172, 1097-4172
Online Access:	Get more information
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