A network model of the molecular organization of chromatin in Drosophila

Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associ...

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Vydané v:Molecular cell Ročník 49; číslo 4; s. 759
Hlavní autori: van Bemmel, Joke G, Filion, Guillaume J, Rosado, Arantxa, Talhout, Wendy, de Haas, Marcel, van Welsem, Tibor, van Leeuwen, Fred, van Steensel, Bas
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 21.02.2013
Predmet:
Animals
Bayes Theorem
Binding Sites
Cell Line
Chromatin - metabolism
Chromosomes, Insect - metabolism
DNA Repair
DNA Replication
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Drosophila Proteins - physiology
Gene Expression Regulation
Models, Biological
Molecular Sequence Annotation
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Nuclear Proteins - physiology
Principal Component Analysis
Protein Binding
Protein Interaction Mapping
Protein Interaction Maps
Protein Processing, Post-Translational
ISSN:1097-4164, 1097-4164
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Popis
Shrnutí:Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associated with chromatin, which all display specific genomic binding patterns. Bayesian network modeling of the binding profiles of these and 70 known chromatin components yields a detailed blueprint of the in vivo chromatin protein network. We demonstrate functional compartmentalization of this network, and predict functions for most of the previously unknown chromatin proteins, including roles in DNA replication and repair, and gene activation and repression.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1097-4164
1097-4164
DOI:10.1016/j.molcel.2013.01.040