Best practices in metabarcoding of fungi: From experimental design to results

The development of high‐throughput sequencing (HTS) technologies has greatly improved our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems. Here we provide an overview of current best practices in metabarcoding analysis of fungal communities, from experimen...

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Bibliographic Details
Published in:	Molecular ecology Vol. 31; no. 10; pp. 2769 - 2795
Main Authors:	Tedersoo, Leho, Bahram, Mohammad, Zinger, Lucie, Nilsson, R. Henrik, Kennedy, Peter G., Yang, Teng, Anslan, Sten, Mikryukov, Vladimir
Format:	Journal Article
Language:	English
Published:	England Blackwell Publishing Ltd 01.05.2022
Subjects:	bayesian Best practice Biochemistry & Molecular Biology biodiversity bioinformatics classifier community ecology Community structure Computer applications Design of experiments DNA barcoding Ecological function Ecology Ecosystem recovery ecosystems ectomycorrhizal fungi Ekologi Environmental Sciences & Ecology Eukaryotes eukaryotic cells Evolutionary Biology experiment setup Experimental design Fungi global change internal transcribed spacer its2 region microbial communities microbiome Microbiomes Molecular Biology molecular identification Molekylärbiologi ribosomal-rna sequence-based identification soil DNA statistical analyses Taxonomy statistical analyses biodiversity experiment setup molecular identification bioinformatics community ecology
ISSN:	0962-1083, 1365-294X, 1365-294X
Online Access:	Get full text
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