Broad anti-SARS-CoV-2 antibody immunity induced by heterologous ChAdOx1/mRNA-1273 vaccination

Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy. We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-specific serological and memory B cell (MBC) responses in individuals who received either homolo...

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Vydáno v:Science (American Association for the Advancement of Science) Ročník 375; číslo 6584; s. 1041
Hlavní autoři: Kaku, Chengzi I, Champney, Elizabeth R, Normark, Johan, Garcia, Marina, Johnson, Carl E, Ahlm, Clas, Christ, Wanda, Sakharkar, Mrunal, Ackerman, Margaret E, Klingström, Jonas, Forsell, Mattias N E, Walker, Laura M
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 04.03.2022
Témata:
2019-nCoV Vaccine mRNA-1273 - administration & dosage
2019-nCoV Vaccine mRNA-1273 - immunology
Adult
Antibodies, Monoclonal - immunology
Antibodies, Neutralizing - blood
Antibodies, Neutralizing - immunology
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antibody Specificity
ChAdOx1 nCoV-19 - administration & dosage
ChAdOx1 nCoV-19 - immunology
COVID-19 Vaccines - immunology
Female
Humans
Immunization Schedule
Immunization, Secondary
Immunogenicity, Vaccine
Male
Memory B Cells - immunology
Middle Aged
Protein Conformation
Protein Domains
SARS-CoV-2 - immunology
Spike Glycoprotein, Coronavirus - chemistry
Spike Glycoprotein, Coronavirus - immunology
ISSN:1095-9203, 1095-9203
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Shrnutí:Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy. We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1:ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) prime-boost vaccination. Heterologous messenger RNA (mRNA) booster immunization induced higher serum neutralizing antibody and MBC responses against SARS-CoV-2 variants of concern (VOCs) compared with that of homologous ChAdOx1 boosting. Specificity mapping of circulating B cells revealed that mRNA-1273 boost immunofocused ChAdOx1-primed responses onto epitopes expressed on prefusion-stabilized S. Monoclonal antibodies isolated from mRNA-1273-boosted participants displayed overall higher binding affinities and increased breadth of reactivity against VOCs relative to those isolated from ChAdOx1-boosted individuals. Overall, the results provide molecular insight into the enhanced quality of the B cell response induced after heterologous mRNA booster vaccination.
Bibliografie:ObjectType-Article-1
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.abn2688