Spatial and temporal gene expression patterns during early human odontogenesis process

Studies on odontogenesis are of great importance to treat dental abnormalities and tooth loss. However, the odontogenesis process was poorly studied in humans, especially at the early developmental stages. Here, we combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) to establi...

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Published in:Frontiers in bioengineering and biotechnology Vol. 12; p. 1437426
Main Authors: Yu, Yejia, Wang, Kun, Wang, Zhuo, Cai, Haoyang, Liao, Chengcheng, Wu, Yutao, Zhang, Jingyi, Tian, Weidong, Liao, Li
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media SA 16.07.2024
Frontiers Media S.A
Subjects:
Abortion
Angiogenesis
Axonogenesis
Bioengineering and Biotechnology
Data analysis
Developmental stages
Embryos
Epithelium
Ethanol
Extracellular matrix
Gene expression
Growth factors
human deciduous tooth germ
laser-capture microdissection
Lasers
Mesenchyme
Odontogenesis
Ossification
RNA sequencing
species comparison
Teeth
Transcription factors
Transcriptomics
China
RNA sequencing
laser-capture microdissection
odontogenesis
human deciduous tooth germ
species comparison
ISSN:2296-4185, 2296-4185
Online Access:Get full text
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Summary:Studies on odontogenesis are of great importance to treat dental abnormalities and tooth loss. However, the odontogenesis process was poorly studied in humans, especially at the early developmental stages. Here, we combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) to establish a spatiotemporal transcriptomic investigation for human deciduous tooth germs at the crucial developmental stage to offer new perspectives to understand tooth development and instruct tooth regeneration. Several hallmark events, including angiogenesis, ossification, axonogenesis, and extracellular matrix (ECM) organization, were identified during odontogenesis in human dental epithelium and mesenchyme from the cap stage to the early bell stage. ECM played an essential role in the shift of tooth-inductive capability. Species comparisons demonstrated these hallmark events both in humans and mice. This study reveals the hallmark events during odontogenesis, enriching the transcriptomic research on human tooth development at the early stage.
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Xujun Qiu, Karlsruhe Institute of Technology (KIT), Germany
These authors have contributed equally to this work and share first authorship
Xue Xiong, The University of Hong Kong, Hong Kong SAR, China
Weichen Song, Shanghai Jiao Tong University, China
Md Shaifur Rahman, Atomic Energy Research Establishment, Bangladesh
Bingdong Sui, Air Force Medical University, China
Reviewed by: Jie Li, Chongqing Medical University, China
Edited by: Qianju Wu, Xiamen Stomatological Hospital, China
ISSN:2296-4185
2296-4185
DOI:10.3389/fbioe.2024.1437426