Spatial and temporal gene expression patterns during early human odontogenesis process
Studies on odontogenesis are of great importance to treat dental abnormalities and tooth loss. However, the odontogenesis process was poorly studied in humans, especially at the early developmental stages. Here, we combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) to establi...
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| Published in: | Frontiers in bioengineering and biotechnology Vol. 12; p. 1437426 |
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| Language: | English |
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| Abstract | Studies on odontogenesis are of great importance to treat dental abnormalities and tooth loss. However, the odontogenesis process was poorly studied in humans, especially at the early developmental stages. Here, we combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) to establish a spatiotemporal transcriptomic investigation for human deciduous tooth germs at the crucial developmental stage to offer new perspectives to understand tooth development and instruct tooth regeneration. Several hallmark events, including angiogenesis, ossification, axonogenesis, and extracellular matrix (ECM) organization, were identified during odontogenesis in human dental epithelium and mesenchyme from the cap stage to the early bell stage. ECM played an essential role in the shift of tooth-inductive capability. Species comparisons demonstrated these hallmark events both in humans and mice. This study reveals the hallmark events during odontogenesis, enriching the transcriptomic research on human tooth development at the early stage. |
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| AbstractList | Studies on odontogenesis are of great importance to treat dental abnormalities and tooth loss. However, the odontogenesis process was poorly studied in humans, especially at the early developmental stages. Here, we combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) to establish a spatiotemporal transcriptomic investigation for human deciduous tooth germs at the crucial developmental stage to offer new perspectives to understand tooth development and instruct tooth regeneration. Several hallmark events, including angiogenesis, ossification, axonogenesis, and extracellular matrix (ECM) organization, were identified during odontogenesis in human dental epithelium and mesenchyme from the cap stage to the early bell stage. ECM played an essential role in the shift of tooth-inductive capability. Species comparisons demonstrated these hallmark events both in humans and mice. This study reveals the hallmark events during odontogenesis, enriching the transcriptomic research on human tooth development at the early stage. Studies on odontogenesis are of great importance to treat dental abnormalities and tooth loss. However, the odontogenesis process was poorly studied in humans, especially at the early developmental stages. Here, we combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) to establish a spatiotemporal transcriptomic investigation for human deciduous tooth germs at the crucial developmental stage to offer new perspectives to understand tooth development and instruct tooth regeneration. Several hallmark events, including angiogenesis, ossification, axonogenesis, and extracellular matrix (ECM) organization, were identified during odontogenesis in human dental epithelium and mesenchyme from the cap stage to the early bell stage. ECM played an essential role in the shift of tooth-inductive capability. Species comparisons demonstrated these hallmark events both in humans and mice. This study reveals the hallmark events during odontogenesis, enriching the transcriptomic research on human tooth development at the early stage.Studies on odontogenesis are of great importance to treat dental abnormalities and tooth loss. However, the odontogenesis process was poorly studied in humans, especially at the early developmental stages. Here, we combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) to establish a spatiotemporal transcriptomic investigation for human deciduous tooth germs at the crucial developmental stage to offer new perspectives to understand tooth development and instruct tooth regeneration. Several hallmark events, including angiogenesis, ossification, axonogenesis, and extracellular matrix (ECM) organization, were identified during odontogenesis in human dental epithelium and mesenchyme from the cap stage to the early bell stage. ECM played an essential role in the shift of tooth-inductive capability. Species comparisons demonstrated these hallmark events both in humans and mice. This study reveals the hallmark events during odontogenesis, enriching the transcriptomic research on human tooth development at the early stage. |
| Author | Yu, Yejia Cai, Haoyang Wu, Yutao Tian, Weidong Liao, Li Wang, Zhuo Liao, Chengcheng Wang, Kun Zhang, Jingyi |
| AuthorAffiliation | 2 Center of Growth, Metabolism and Aging , Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences , Sichuan University , Chengdu , China 1 State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Engineering Research Center of Oral Translational Medicine , Ministry of Education and National Engineering Laboratory for Oral Regenerative Medicine , West China Hospital of Stomatology , Sichuan University , Chengdu , China 3 Chengdu Shiliankangjian Biotechnology Co., Ltd. , Chengdu , China |
| AuthorAffiliation_xml | – name: 3 Chengdu Shiliankangjian Biotechnology Co., Ltd. , Chengdu , China – name: 1 State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Engineering Research Center of Oral Translational Medicine , Ministry of Education and National Engineering Laboratory for Oral Regenerative Medicine , West China Hospital of Stomatology , Sichuan University , Chengdu , China – name: 2 Center of Growth, Metabolism and Aging , Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences , Sichuan University , Chengdu , China |
| Author_xml | – sequence: 1 givenname: Yejia surname: Yu fullname: Yu, Yejia – sequence: 2 givenname: Kun surname: Wang fullname: Wang, Kun – sequence: 3 givenname: Zhuo surname: Wang fullname: Wang, Zhuo – sequence: 4 givenname: Haoyang surname: Cai fullname: Cai, Haoyang – sequence: 5 givenname: Chengcheng surname: Liao fullname: Liao, Chengcheng – sequence: 6 givenname: Yutao surname: Wu fullname: Wu, Yutao – sequence: 7 givenname: Jingyi surname: Zhang fullname: Zhang, Jingyi – sequence: 8 givenname: Weidong surname: Tian fullname: Tian, Weidong – sequence: 9 givenname: Li surname: Liao fullname: Liao, Li |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39081334$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1177/0022034514523988 10.1038/nature06153 10.1039/c5mb00663e 10.1186/s13059-014-0550-8 10.1016/j.devcel.2023.07.013 10.1242/dev.202043 10.1186/s12864-023-09140-8 10.1016/j.xinn.2021.100141 10.1038/sj.cr.7290299 10.1002/jcp.24532 10.1111/cpr.12479 10.1016/j.celrep.2022.111737 10.1242/dev.103.Supplement.155 10.1387/ijdb.103214ec 10.1111/joa.12950 10.1038/ng0494-348 10.1007/978-1-4939-7213-5_6 10.1101/gad.12.17.2735 10.1038/s41598-017-14185-3 10.1016/S0925-4773(97)00115-9 10.1016/0092-8674(93)90678-j 10.1186/s13578-021-00579-4 10.3389/fphys.2012.00107 10.1126/science.278.5342.1481 10.1016/j.cell.2019.01.021 10.1126/sciadv.abb0119 10.1038/s41467-020-18512-7 10.1371/journal.pone.0072614 10.1101/gad.13.23.3136 10.1016/0003-9969(87)90055-0 10.1016/j.gde.2019.07.020 10.1093/bioinformatics/btt656 10.1038/nprot.2017.003 10.1242/dev.24.1.159 |
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| Copyright | Copyright © 2024 Yu, Wang, Wang, Cai, Liao, Wu, Zhang, Tian and Liao. 2024. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright © 2024 Yu, Wang, Wang, Cai, Liao, Wu, Zhang, Tian and Liao. 2024 Yu, Wang, Wang, Cai, Liao, Wu, Zhang, Tian and Liao |
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| Keywords | RNA sequencing laser-capture microdissection odontogenesis human deciduous tooth germ species comparison |
| Language | English |
| License | Copyright © 2024 Yu, Wang, Wang, Cai, Liao, Wu, Zhang, Tian and Liao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Xujun Qiu, Karlsruhe Institute of Technology (KIT), Germany These authors have contributed equally to this work and share first authorship Xue Xiong, The University of Hong Kong, Hong Kong SAR, China Weichen Song, Shanghai Jiao Tong University, China Md Shaifur Rahman, Atomic Energy Research Establishment, Bangladesh Bingdong Sui, Air Force Medical University, China Reviewed by: Jie Li, Chongqing Medical University, China Edited by: Qianju Wu, Xiamen Stomatological Hospital, China |
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| SubjectTerms | Abortion Angiogenesis Axonogenesis Bioengineering and Biotechnology Data analysis Developmental stages Embryos Epithelium Ethanol Extracellular matrix Gene expression Growth factors human deciduous tooth germ laser-capture microdissection Lasers Mesenchyme Odontogenesis Ossification RNA sequencing species comparison Teeth Transcription factors Transcriptomics |
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| Title | Spatial and temporal gene expression patterns during early human odontogenesis process |
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