Use of monoclonal antibodies to identify serotypes of enterovirus isolates
Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of enteroviruses is cell culture isolation, followed by serotype identification by neutralization assay. These procedures are time-consuming an...
Uloženo v:
| Vydáno v: | Journal of clinical microbiology Ročník 36; číslo 7; s. 1877 |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
01.07.1998
|
| Témata: | |
| ISSN: | 0095-1137 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of enteroviruses is cell culture isolation, followed by serotype identification by neutralization assay. These procedures are time-consuming and expensive. Rapid serotype identification of enteroviruses is important in differentiating nonpoliovirus enterovirus pathogens from vaccine strain polioviruses that can be shed for some time after vaccination. In the present investigation, we evaluated a rapid method for serotype identification of enteroviruses by indirect immunofluorescence assay (IFA) using commercially available monoclonal antibodies for polioviruses, coxsackieviruses type B, and six serotypes of commonly circulating echoviruses. Of 291 isolates of enteroviruses included in the study, 95 were polioviruses and 196 were nonpoliovirus enteroviruses. Two hundred thirty-four of these (38 polioviruses and 196 nonpoliovirus enteroviruses) were consecutively grown in the laboratory over a 5-year period. IFA identified the serotypes of 74% of the consecutive isolates and 71% of all enterovirus isolates by yielding a positive staining result. The levels of agreement in the identification of the enterovirus group between IFA and neutralization tests were 92% for consecutively grown isolates and 85% for all enterovirus isolates. The sensitivity of the IFA for the detection of viruses for which specific monoclonal antibodies were applied was 73% for polioviruses, 85% for coxsackieviruses type B, and 94% for echoviruses. Specificity was near 100% for polioviruses and coxsackieviruses type B and 94% for echoviruses. We conclude that IFA can be helpful as a preliminary test for serotype identification of enteroviruses. The results are most accurate when the test identifies the isolate as a poliovirus. |
|---|---|
| AbstractList | Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of enteroviruses is cell culture isolation, followed by serotype identification by neutralization assay. These procedures are time-consuming and expensive. Rapid serotype identification of enteroviruses is important in differentiating nonpoliovirus enterovirus pathogens from vaccine strain polioviruses that can be shed for some time after vaccination. In the present investigation, we evaluated a rapid method for serotype identification of enteroviruses by indirect immunofluorescence assay (IFA) using commercially available monoclonal antibodies for polioviruses, coxsackieviruses type B, and six serotypes of commonly circulating echoviruses. Of 291 isolates of enteroviruses included in the study, 95 were polioviruses and 196 were nonpoliovirus enteroviruses. Two hundred thirty-four of these (38 polioviruses and 196 nonpoliovirus enteroviruses) were consecutively grown in the laboratory over a 5-year period. IFA identified the serotypes of 74% of the consecutive isolates and 71% of all enterovirus isolates by yielding a positive staining result. The levels of agreement in the identification of the enterovirus group between IFA and neutralization tests were 92% for consecutively grown isolates and 85% for all enterovirus isolates. The sensitivity of the IFA for the detection of viruses for which specific monoclonal antibodies were applied was 73% for polioviruses, 85% for coxsackieviruses type B, and 94% for echoviruses. Specificity was near 100% for polioviruses and coxsackieviruses type B and 94% for echoviruses. We conclude that IFA can be helpful as a preliminary test for serotype identification of enteroviruses. The results are most accurate when the test identifies the isolate as a poliovirus.Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of enteroviruses is cell culture isolation, followed by serotype identification by neutralization assay. These procedures are time-consuming and expensive. Rapid serotype identification of enteroviruses is important in differentiating nonpoliovirus enterovirus pathogens from vaccine strain polioviruses that can be shed for some time after vaccination. In the present investigation, we evaluated a rapid method for serotype identification of enteroviruses by indirect immunofluorescence assay (IFA) using commercially available monoclonal antibodies for polioviruses, coxsackieviruses type B, and six serotypes of commonly circulating echoviruses. Of 291 isolates of enteroviruses included in the study, 95 were polioviruses and 196 were nonpoliovirus enteroviruses. Two hundred thirty-four of these (38 polioviruses and 196 nonpoliovirus enteroviruses) were consecutively grown in the laboratory over a 5-year period. IFA identified the serotypes of 74% of the consecutive isolates and 71% of all enterovirus isolates by yielding a positive staining result. The levels of agreement in the identification of the enterovirus group between IFA and neutralization tests were 92% for consecutively grown isolates and 85% for all enterovirus isolates. The sensitivity of the IFA for the detection of viruses for which specific monoclonal antibodies were applied was 73% for polioviruses, 85% for coxsackieviruses type B, and 94% for echoviruses. Specificity was near 100% for polioviruses and coxsackieviruses type B and 94% for echoviruses. We conclude that IFA can be helpful as a preliminary test for serotype identification of enteroviruses. The results are most accurate when the test identifies the isolate as a poliovirus. Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of enteroviruses is cell culture isolation, followed by serotype identification by neutralization assay. These procedures are time-consuming and expensive. Rapid serotype identification of enteroviruses is important in differentiating nonpoliovirus enterovirus pathogens from vaccine strain polioviruses that can be shed for some time after vaccination. In the present investigation, we evaluated a rapid method for serotype identification of enteroviruses by indirect immunofluorescence assay (IFA) using commercially available monoclonal antibodies for polioviruses, coxsackieviruses type B, and six serotypes of commonly circulating echoviruses. Of 291 isolates of enteroviruses included in the study, 95 were polioviruses and 196 were nonpoliovirus enteroviruses. Two hundred thirty-four of these (38 polioviruses and 196 nonpoliovirus enteroviruses) were consecutively grown in the laboratory over a 5-year period. IFA identified the serotypes of 74% of the consecutive isolates and 71% of all enterovirus isolates by yielding a positive staining result. The levels of agreement in the identification of the enterovirus group between IFA and neutralization tests were 92% for consecutively grown isolates and 85% for all enterovirus isolates. The sensitivity of the IFA for the detection of viruses for which specific monoclonal antibodies were applied was 73% for polioviruses, 85% for coxsackieviruses type B, and 94% for echoviruses. Specificity was near 100% for polioviruses and coxsackieviruses type B and 94% for echoviruses. We conclude that IFA can be helpful as a preliminary test for serotype identification of enteroviruses. The results are most accurate when the test identifies the isolate as a poliovirus. |
| Author | Chonmaitree, T Mann, L Rigonan, A S |
| Author_xml | – sequence: 1 givenname: A S surname: Rigonan fullname: Rigonan, A S organization: Department of Pediatrics, University of Texas Medical Branch at Galveston, 77555-0371, USA – sequence: 2 givenname: L surname: Mann fullname: Mann, L – sequence: 3 givenname: T surname: Chonmaitree fullname: Chonmaitree, T |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/9650928$$D View this record in MEDLINE/PubMed |
| BookMark | eNotj0tLw0AYRWdRqW31J4hZuUuc92MpxVepuLHrMJn5BkaSTMwkQv-9Abu6nMvhwt2iVZ96QOie4IoQqh8P-4-KyUpVRCtVEq1JRYzRK7TB2IiSEKau0Tbnb4wJ50Ks0dpIgQ3VG3Q4ZShSKLrUJ9em3raF7afYJB8hF1MqooeFw7nIMKbpPCztoi_dgr9xnHMRc2rtBPkGXQXbZri95A6dXp6_9m_l8fP1ff90LB03aioJ4ywwQ62WwUpuhZOOcgWUBQ_KKw1YCN1Y2zSOaid9MFKpRmIvIHDP6A49_O8OY_qZIU91F7ODtrU9pDnXyhilhVGLeHcR56YDXw9j7Ox4ri_n6R_M017H |
| CitedBy_id | crossref_primary_10_1016_j_ijheh_2011_07_014 crossref_primary_10_1186_s12929_019_0573_2 crossref_primary_10_1038_srep06803 crossref_primary_10_1128_JCM_02393_05 crossref_primary_10_1007_s00216_011_5407_3 crossref_primary_10_1002_jmv_20917 crossref_primary_10_1128_JCM_00479_09 crossref_primary_10_1128_JCM_05828_11 crossref_primary_10_1007_s00705_008_0266_8 crossref_primary_10_1016_j_jcv_2009_05_035 crossref_primary_10_1128_CMR_00002_06 crossref_primary_10_1002_jmv_23372 crossref_primary_10_1016_j_patol_2016_06_003 crossref_primary_10_1046_j_1365_2672_2001_01470_x crossref_primary_10_1016_j_phrp_2015_11_011 crossref_primary_10_1128_JCM_02384_09 crossref_primary_10_1099_jmm_0_47799_0 crossref_primary_10_1016_j_jviromet_2010_09_013 crossref_primary_10_1128_JCM_01114_07 crossref_primary_10_1002_jcla_2063 crossref_primary_10_1089_mab_2013_0033 crossref_primary_10_1097_INF_0000000000002985 crossref_primary_10_1093_infdis_jir490 crossref_primary_10_1111_j_1348_0421_2004_tb03557_x crossref_primary_10_1586_14737159_7_4_419 crossref_primary_10_1016_S0166_0934_01_00437_2 crossref_primary_10_3390_microorganisms12020367 crossref_primary_10_1016_j_mcp_2004_10_006 crossref_primary_10_1016_j_meegid_2011_05_010 crossref_primary_10_1007_s13206_018_2301_5 crossref_primary_10_1016_j_jviromet_2009_12_016 crossref_primary_10_1128_JCM_40_12_4554_4560_2002 crossref_primary_10_1002_jcla_7 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1128/JCM.36.7.1877-1881.1998 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine Biology |
| ExternalDocumentID | 9650928 |
| Genre | Research Support, U.S. Gov't, P.H.S Journal Article |
| GrantInformation_xml | – fundername: NIDCD NIH HHS grantid: DC-02620 |
| GroupedDBID | --- .55 .GJ 0R~ 18M 29K 2WC 39C 3O- 4.4 41~ 53G 5GY 5RE 5VS AAYOK ABOCM ABPPZ ACGFO ADBBV AENEX AGCDD AGVNZ AI. ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW CGR CS3 CUY CVF D-I DIK DU5 E3Z EBS ECM EIF EJD F5P FRP GX1 H13 HF~ HYE HZ~ H~9 KQ8 L7B NPM O9- OHT OK1 P2P P6G PKN RHF RHI RNS RPM RSF TR2 UCJ VH1 W8F WHG WOQ X7M YIF ZCA ZGI ZXP ~KM 7X8 AAGFI |
| ID | FETCH-LOGICAL-c497t-1343f392a86fa64a5c6c247e23fde7d78e0558baabbc28c6df9677b60d5ef4d32 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 38 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000074155400009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0095-1137 |
| IngestDate | Thu Oct 02 06:46:43 EDT 2025 Wed Feb 19 01:19:39 EST 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 7 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c497t-1343f392a86fa64a5c6c247e23fde7d78e0558baabbc28c6df9677b60d5ef4d32 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://jcm.asm.org/content/jcm/36/7/1877.full.pdf |
| PMID | 9650928 |
| PQID | 79978597 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_79978597 pubmed_primary_9650928 |
| PublicationCentury | 1900 |
| PublicationDate | 1998-07-01 |
| PublicationDateYYYYMMDD | 1998-07-01 |
| PublicationDate_xml | – month: 07 year: 1998 text: 1998-07-01 day: 01 |
| PublicationDecade | 1990 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Journal of clinical microbiology |
| PublicationTitleAlternate | J Clin Microbiol |
| PublicationYear | 1998 |
| References | 6278169 - JAMA. 1982 Apr 2;247(13):1843-7 7814507 - J Clin Microbiol. 1994 Oct;32(10):2590-2 2456458 - Mol Cell Probes. 1987 Jun;1(2):169-76 2852672 - J Clin Microbiol. 1988 Dec;26(12):2576-80 7769037 - J Virol Methods. 1995 Mar;52(1-2):35-9 15566790 - Clin Diagn Virol. 1995 Jan;3(1):83-93 3031978 - Am J Dis Child. 1987 Apr;141(4):454-7 9384344 - Pediatr Infect Dis J. 1997 Nov;16(11):1086-7 2644021 - Clin Microbiol Rev. 1989 Jan;2(1):1-14 2157735 - J Clin Microbiol. 1990 Mar;28(3):438-42 9399539 - J Clin Microbiol. 1997 Dec;35(12):3301-2 7679404 - J Clin Microbiol. 1993 Feb;31(2):395-9 |
| References_xml | – reference: 9384344 - Pediatr Infect Dis J. 1997 Nov;16(11):1086-7 – reference: 6278169 - JAMA. 1982 Apr 2;247(13):1843-7 – reference: 7679404 - J Clin Microbiol. 1993 Feb;31(2):395-9 – reference: 7769037 - J Virol Methods. 1995 Mar;52(1-2):35-9 – reference: 15566790 - Clin Diagn Virol. 1995 Jan;3(1):83-93 – reference: 2852672 - J Clin Microbiol. 1988 Dec;26(12):2576-80 – reference: 2644021 - Clin Microbiol Rev. 1989 Jan;2(1):1-14 – reference: 2157735 - J Clin Microbiol. 1990 Mar;28(3):438-42 – reference: 9399539 - J Clin Microbiol. 1997 Dec;35(12):3301-2 – reference: 3031978 - Am J Dis Child. 1987 Apr;141(4):454-7 – reference: 7814507 - J Clin Microbiol. 1994 Oct;32(10):2590-2 – reference: 2456458 - Mol Cell Probes. 1987 Jun;1(2):169-76 |
| SSID | ssj0014455 |
| Score | 1.7801601 |
| Snippet | Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 1877 |
| SubjectTerms | Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Coxsackievirus Infections - diagnosis Coxsackievirus Infections - virology Echovirus Infections - diagnosis Echovirus Infections - virology Enterovirus - classification Enterovirus - immunology Enterovirus - isolation & purification Enterovirus B, Human - classification Enterovirus B, Human - isolation & purification Enterovirus Infections - diagnosis Enterovirus Infections - virology Evaluation Studies as Topic False Negative Reactions False Positive Reactions Fluorescent Antibody Technique, Indirect Humans Neutralization Tests Poliomyelitis - diagnosis Poliomyelitis - virology Poliovirus - classification Poliovirus - isolation & purification Predictive Value of Tests Sensitivity and Specificity Serotyping |
| Title | Use of monoclonal antibodies to identify serotypes of enterovirus isolates |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/9650928 https://www.proquest.com/docview/79978597 |
| Volume | 36 |
| WOSCitedRecordID | wos000074155400009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEB5848V38e0evKYm2c3OBgQRUaTY0oNCbyX7goI0tVHBf-9OkuJJPHjJaRfCZDL77Xwz8wFcotDceG4j5zBcUFDZKC9SHT5IGvB5ppW09RDXJxwM1GiUD5fgetELQ2WVi5hYB2pbGsqRX2Ee7jsB_d7M3iLSjCJutRXQWIZVHoAMFXTh6IdDECJr9AvyLEoSjm11VwjIV727fpfLLnYThRglSiXUtad-R5n1afOw_b_33IGtFmWy28YtdmHJTfdgvdGd_NqDjX7LqO9D76VyrPQsbCzNK8FyFmw90SVVF7L3kk3qTl7_xYKvlpSwrWg5TfKkZMT8o2KT4L6EWA_g5eH--e4xavUVIiNyJBV6wX3AR4WSvpCiyIw0qUCXcm8dWlQuzjKli0Jrkyojrc8lopaxzZwXlqcdWJmWU3cILBYY54W2PkYrUDrlFSdS2wf8QfNljuBiYa1x8F8iJYqpKz-q8cJeR9BpDD6eNWM2xjkN90vV8Z9bT2Cz6RSkItpTWPXhx3VnsGY-3yfV_Lz2ivAcDPvfBsHBGg |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Use+of+monoclonal+antibodies+to+identify+serotypes+of+enterovirus+isolates&rft.jtitle=Journal+of+clinical+microbiology&rft.au=Rigonan%2C+A+S&rft.au=Mann%2C+L&rft.au=Chonmaitree%2C+T&rft.date=1998-07-01&rft.issn=0095-1137&rft.volume=36&rft.issue=7&rft.spage=1877&rft_id=info:doi/10.1128%2FJCM.36.7.1877-1881.1998&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0095-1137&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0095-1137&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0095-1137&client=summon |