Moderate Exercise Allows for shorter Recovery Time in Critical Limb Ischemia

Whether and how moderate exercise might allow for accelerated limb recovery in chronic critical limb ischemia (CLI) remains to be determined. Chronic CLI was surgically induced in mice, and the effect of moderate exercise (training five times per week over a 3-week period) was investigated. Tissue d...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Frontiers in physiology Jg. 8; S. 523
Hauptverfasser: Lejay, Anne, Laverny, Gilles, Paradis, Stéphanie, Schlagowski, Anna-Isabel, Charles, Anne-Laure, Singh, François, Zoll, Joffrey, Thaveau, Fabien, Lonsdorfer, Evelyne, Dufour, Stéphane, Favret, Fabrice, Wolff, Valérie, Metzger, Daniel, Chakfe, Nabil, Geny, Bernard
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland Frontiers 25.07.2017
Frontiers Media S.A
Schlagworte:
exercise
Human health and pathology
ischemia
Life Sciences
mitochondria
muscle
oxidative stress
peripheral arterial disease
Physiology
Tissues and Organs
mitochondria
muscle
exercise
peripheral arterial disease
oxidative stress
ischemia
sarcopenia
ISSN:1664-042X, 1664-042X
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Whether and how moderate exercise might allow for accelerated limb recovery in chronic critical limb ischemia (CLI) remains to be determined. Chronic CLI was surgically induced in mice, and the effect of moderate exercise (training five times per week over a 3-week period) was investigated. Tissue damages and functional scores were assessed on the 4th, 6th, 10th, 20th, and 30th day after surgery. Mice were sacrificed 48 h after the last exercise session in order to assess muscle structure, mitochondrial respiration, calcium retention capacity, oxidative stress and transcript levels of genes encoding proteins controlling mitochondrial functions (PGC1α, PGC1β, NRF1) and anti-oxidant defenses markers (SOD1, SOD2, catalase). CLI resulted in tissue damages and impaired functional scores. Mitochondrial respiration and calcium retention capacity were decreased in the ischemic limb of the non-exercised group (V = 7.11 ± 1.14 vs. 9.86 ± 0.86 mmol 02/min/g dw, < 0.001; CRC = 7.01 ± 0.97 vs. 11.96 ± 0.92 microM/mg dw, < 0.001, respectively). Moderate exercise reduced tissue damages, improved functional scores, and restored mitochondrial respiration and calcium retention capacity in the ischemic limb (V = 9.75 ± 1.00 vs. 9.82 ± 0.68 mmol 02/min/g dw; CRC = 11.36 ± 1.33 vs. 12.01 ± 1.24 microM/mg dw, respectively). Exercise also enhanced the transcript levels of PGC1α, PGC1β, NRF1, as well as SOD1, SOD2, and catalase. Moderate exercise restores mitochondrial respiration and calcium retention capacity, and it has beneficial functional effects in chronic CLI, likely by stimulating reactive oxygen species-induced biogenesis and anti-oxidant defenses. These data support further development of exercise therapy even in advanced peripheral arterial disease.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMCID: PMC5524729
Edited by: Agustín Guerrero-Hernández, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV), Mexico
Reviewed by: Pasquale Pagliaro, University of Turin, Italy; Adán Dagnino-Acosta, University of Colima, Mexico
This article was submitted to Vascular Physiology, a section of the journal Frontiers in Physiology
ISSN:1664-042X
1664-042X
DOI:10.3389/fphys.2017.00523