A Structure-Based Mechanism for DNA Entry into the Cohesin Ring

Despite key roles in sister chromatid cohesion and chromosome organization, the mechanism by which cohesin rings are loaded onto DNA is still unknown. Here we combine biochemical approaches and cryoelectron microscopy (cryo-EM) to visualize a cohesin loading intermediate in which DNA is locked betwe...

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Vydané v:	Molecular cell Ročník 79; číslo 6; s. 917
Hlavní autori:	Higashi, Torahiko L, Eickhoff, Patrik, Sousa, Joana S, Locke, Julia, Nans, Andrea, Flynn, Helen R, Snijders, Ambrosius P, Papageorgiou, George, O'Reilly, Nicola, Chen, Zhuo A, O'Reilly, Francis J, Rappsilber, Juri, Costa, Alessandro, Uhlmann, Frank
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 17.09.2020
Predmet:	Adenosine Triphosphatases - genetics Cell Cycle Proteins - genetics Cell Cycle Proteins - ultrastructure Chromatids - genetics Chromatids - ultrastructure Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - ultrastructure Chromosome Segregation - genetics Cohesins Cryoelectron Microscopy DNA - genetics DNA - ultrastructure Nucleic Acid Conformation Protein Conformation Saccharomyces cerevisiae - ultrastructure Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - ultrastructure Sister Chromatid Exchange - genetics ABC-ATPase cohesin SMC complexes chromosome segregation sister chromatid cohesion S. pombe DNA-protein crosslink mass spectrometry Mis4/Scc2/NIPBL DNA loop extrusion cryo-electron microscopy
ISSN:	1097-4164, 1097-4164
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Shrnutí:	Despite key roles in sister chromatid cohesion and chromosome organization, the mechanism by which cohesin rings are loaded onto DNA is still unknown. Here we combine biochemical approaches and cryoelectron microscopy (cryo-EM) to visualize a cohesin loading intermediate in which DNA is locked between two gates that lead into the cohesin ring. Building on this structural framework, we design experiments to establish the order of events during cohesin loading. In an initial step, DNA traverses an N-terminal kleisin gate that is first opened upon ATP binding and then closed as the cohesin loader locks the DNA against the ATPase gate. ATP hydrolysis will lead to ATPase gate opening to complete DNA entry. Whether DNA loading is successful or results in loop extrusion might be dictated by a conserved kleisin N-terminal tail that guides the DNA through the kleisin gate. Our results establish the molecular basis for cohesin loading onto DNA.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1097-4164 1097-4164
DOI:	10.1016/j.molcel.2020.07.013