Novel groups of Gammaproteobacteria catalyse sulfur oxidation and carbon fixation in a coastal, intertidal sediment

The oxidation of hydrogen sulfide is essential to sulfur cycling in marine habitats. However, the role of microbial sulfur oxidation in marine sediments and the microorganisms involved are largely unknown, except for the filamentous, mat-forming bacteria. In this study we explored the diversity, abu...

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Vydáno v:Environmental microbiology Ročník 13; číslo 3; s. 758 - 774
Hlavní autoři: Lenk, Sabine, Arnds, Julia, Zerjatke, Katrice, Musat, Niculina, Amann, Rudolf, Mußmann, Marc
Médium: Journal Article
Jazyk:angličtina
Vydáno: Oxford, UK Blackwell Publishing Ltd 01.03.2011
Témata:
adenylyl-sulfate reductase
bacteria
Biocatalysis
carbon
Carbon Cycle
classification
DNA libraries
endosymbionts
fluorescence in situ hybridization
gamma-Proteobacteria
Gammaproteobacteria
Gammaproteobacteria - classification
Gammaproteobacteria - genetics
Gammaproteobacteria - metabolism
genes
Genes, rRNA
genetics
Geologic Sediments
Geologic Sediments - microbiology
habitats
hydrogen sulfide
Hydrogensulfite Reductase
Hydrogensulfite Reductase - genetics
marine sediments
metabolism
microbiology
molecular cloning
Molecular Sequence Data
nucleotide sequences
Oligobrachia
oxidation
Oxidation-Reduction
Phylogeny
primary productivity
ribosomal RNA
RNA, Ribosomal, 16S
RNA, Ribosomal, 16S - genetics
sequence analysis
Sulfides
Sulfides - metabolism
sulfur
Sulfur - metabolism
ISSN:1462-2912, 1462-2920, 1462-2920
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Shrnutí:The oxidation of hydrogen sulfide is essential to sulfur cycling in marine habitats. However, the role of microbial sulfur oxidation in marine sediments and the microorganisms involved are largely unknown, except for the filamentous, mat-forming bacteria. In this study we explored the diversity, abundance and activity of sulfur-oxidizing prokaryotes (SOP) in sulfidic intertidal sediments using 16S rRNA and functional gene sequence analyses, fluorescence in situ hybridization (FISH) and microautoradiography. The 16S rRNA gene analysis revealed that distinct clades of uncultured Gammaproteobacteria are important SOP in the tidal sediments. This was supported by the dominance of gammaproteobacterial sequences in clone libraries of genes encoding the reverse dissimilatory sulfite reductase (rDSR) and the adenosine phosphosulfate reductase (APR). Numerous sequences of all three genes grouped with uncultured autotrophic SOP. Accordingly, Gammaproteobacteria accounted for 40-70% of all ¹⁴CO₂-incorporating cells in surface sediments as shown by microautoradiography. Furthermore, phylogenetic analysis of all three genes consistently suggested a discrete population of SOP that was most closely related to the sulfur-oxidizing endosymbionts of the tubeworm Oligobrachia spp. FISH showed that members of this population (WS-Gam209 group) were abundant, reaching up to 1.3 × 10⁸ cells ml⁻¹ (4.6% of all cells). Approximately 25% of this population incorporated CO₂, consistent with a chemolithoautotrophic metabolism most likely based on sulfur oxidation. Thus, we hypothesize that novel, gammaproteobacterial SOP attached to sediment particles may play a more important role for sulfide removal and primary production in marine sediments than previously assumed.
Bibliografie:http://dx.doi.org/10.1111/j.1462-2920.2010.02380.x
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ArticleID:EMI2380
ark:/67375/WNG-18CS0J1Q-T
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1462-2912
1462-2920
1462-2920
DOI:10.1111/j.1462-2920.2010.02380.x