Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR

Photothermal conversion-based quantitative polymerase chain reaction (qPCR) is a fast, sensitive, and accurate method to diagnose infectious diseases. However, they have bottlenecks in test throughput scalability, cumbersome oil cover, and a lack of multi-target capability. Here, the authors present...

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Veröffentlicht in:Microsystems & nanoengineering Jg. 11; H. 1; S. 143 - 11
Hauptverfasser: Shrestha, Kiran, Kim, Seongryeong, Han, Jiyeon, Zhang, Meng, Parajuli, Sajjan, Cho, Gyoujin
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 15.07.2025
Springer Nature B.V
Nature Publishing Group
Schlagworte:
639/166/987
639/624/1075/1083
Coronaviruses
COVID-19
Disease control
Disease transmission
Engineering
Fluorescent dyes
Fluorescent indicators
Infectious diseases
Measurement methods
Photothermal conversion
Polydimethylsiloxane
Polymerase chain reaction
Reagents
Respiratory diseases
Reverse transcription
Semiconductors
Sensors
Severe acute respiratory syndrome coronavirus 2
Temperature
Temperature control
Thermal cycling
Viral diseases
ISSN:2055-7434, 2096-1030, 2055-7434
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Zusammenfassung:Photothermal conversion-based quantitative polymerase chain reaction (qPCR) is a fast, sensitive, and accurate method to diagnose infectious diseases. However, they have bottlenecks in test throughput scalability, cumbersome oil cover, and a lack of multi-target capability. Here, the authors present an infectious disease diagnostic device with rapid photothermal conversion-based efficient reverse transcription (RT)-qPCR assays on a multi-target chip (idream-qPCR). The authors innovate an off-axis mirror-based three-channel fluorescence intensity measurement method, enabling concurrent non-contact temperature control of 16 mini-well reaction chambers for qPCRs without the necessity of actuating parts. A transparent adhesive film on a graphite mixed polydimethylsiloxane (PDMS)-based PCR chip with mini-wells avoids contamination and bubbles to achieve 16 RT-qPCRs (40 photothermal cycles) within 17 min. Finally, idream-qPCR is validated by amplifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N1 72 bp, RdRP 100 bp, and E 113 bp genes using Fluorescein amidites (FAM), Carboxytetramethylrhodamine (TAMRA), and Cyanine5 (CY5) fluorescent dyes, respectively, with 102.5% efficiency and a limit-of-detection (LoD) equivalent to 0.85 copies/µL. idream-qPCR can be efficiently used to prevent the spread of infectious diseases.
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ISSN:2055-7434
2096-1030
2055-7434
DOI:10.1038/s41378-025-00972-w