Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis
The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical sampl...
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| Vydané v: | Journal of clinical microbiology Ročník 44; číslo 7; s. 2601 |
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| Hlavní autori: | , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
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United States
01.07.2006
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| ISSN: | 0095-1137 |
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| Abstract | The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found. |
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| AbstractList | The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found. The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found. |
| Author | Rogers, G B Connett, G J Kehagia, V Bruce, K D Serisier, D J Carroll, M P Hockey, P M Jones, G |
| Author_xml | – sequence: 1 givenname: G B surname: Rogers fullname: Rogers, G B organization: King's College London, Pharmaceutical Science Research Division, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, and Cystic Fibrosis Unit, Southampton University Hospital, United Kingdom – sequence: 2 givenname: M P surname: Carroll fullname: Carroll, M P – sequence: 3 givenname: D J surname: Serisier fullname: Serisier, D J – sequence: 4 givenname: P M surname: Hockey fullname: Hockey, P M – sequence: 5 givenname: G surname: Jones fullname: Jones, G – sequence: 6 givenname: V surname: Kehagia fullname: Kehagia, V – sequence: 7 givenname: G J surname: Connett fullname: Connett, G J – sequence: 8 givenname: K D surname: Bruce fullname: Bruce, K D |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16825392$$D View this record in MEDLINE/PubMed |
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| References | 12904354 - J Clin Microbiol. 2003 Aug;41(8):3548-58 12034069 - J Can Dent Assoc. 2002 Jun;68(6):340-5 12488570 - J Med Microbiol. 2003 Jan;52(Pt 1):79-89 9361437 - Appl Environ Microbiol. 1997 Nov;63(11):4516-22 15929792 - Respir Res. 2005;6:49 10440642 - J Periodontol. 1999 Jul;70(7):793-802 15528712 - J Clin Microbiol. 2004 Nov;42(11):5176-83 11371542 - J Bacteriol. 2001 Jun;183(12):3770-83 9722708 - Ann Periodontol. 1998 Jul;3(1):251-6 |
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| SubjectTerms | Adult Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacterial Infections - microbiology Cluster Analysis Cystic Fibrosis - complications Cystic Fibrosis - microbiology DNA, Bacterial - genetics DNA, Ribosomal - genetics Genes, rRNA Humans Lung - microbiology Mouth - microbiology Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sputum - microbiology |
| Title | Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis |
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