Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical sampl...

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Veröffentlicht in:Journal of clinical microbiology Jg. 44; H. 7; S. 2601
Hauptverfasser: Rogers, G B, Carroll, M P, Serisier, D J, Hockey, P M, Jones, G, Kehagia, V, Connett, G J, Bruce, K D
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.07.2006
Schlagworte:
Adult
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacterial Infections - microbiology
Cluster Analysis
Cystic Fibrosis - complications
Cystic Fibrosis - microbiology
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Genes, rRNA
Humans
Lung - microbiology
Mouth - microbiology
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Sputum - microbiology
ISSN:0095-1137
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Zusammenfassung:The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0095-1137
DOI:10.1128/JCM.02282-05